Strategie NMR wyznaczania struktur białek w roztworze

Ejchart, A.

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Tytuł artykułu

Strategie NMR wyznaczania struktur białek w roztworze

Autorzy

Ejchart A.

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Warianty tytułu

NMR strategies for the protein structure determination in solution

Języki publikacji

Abstrakty

A number of reasons have hindered the use of NMR spectroscopy as a tool for the protein structure determination. Recently, the advance in the NMR equipment, spectral techniques and isotope labelling has resulted in an enormous growth of NMR-determined protein structures. After a brief presentation of protein structure and conformation several types of NMR-derived constraints and the characteristic features of chemical shifts in proteins are discussed. Short-range, distance and dihedral angle constraints are valuable, but cumulative errors can appear when succesive constraints are used to determine spatial relationship of remote parts of a protein. Therefore, long-range constraints derived from residual dipolar couplings are highly complementary to the short-range constraints. Modern strategies to the NMR-based protein structure determination depend on the size of studied biomolecules. Small proteins (M_cz< 10 kDa) can be studied with the use of two-dimensional (2D) ¹H NMR techniques. Medium size proteins (M_cz < 30 kDa) require double isotopic labeling ¹⁵N/¹³C and multidimensional (3D and 4D) heteronuclear techniques. There is no well established strategy to the structure determination of large proteins (M_cz > 30 kDa) yet. The most promising approaches take advantage of triple isotopic labeling ¹⁵N/¹³C/²H and the transverse relaxation optimized spectroscopy (TROSY), both resulting in the reduction of signal width.

Słowa kluczowe

spektroskopia NMR struktury białek w roztworach więzy strukturalne NMR

NMR spectroscopy protein structure in solution NMR structural constrains

Wydawca

Polskie Towarzystwo Chemiczne

Czasopismo

Wiadomości Chemiczne

Rocznik

2005

Tom

[Z] 59, 1-2

Strony

23--45

Opis fizyczny

Bibliogr. 46 poz., wykr.

Twórcy

autor

Ejchart A.

Instytut Biochemii i Biofizyki PAN, Pawińskiego 5A, 02-106 Warszawa

Bibliografia

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Typ dokumentu

Bibliografia

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