Wykorzystanie HPLC do oznaczania witamin rozpuszczalnych w tłuszczach

• Autorzy: Wieliński S. , Olszanowski A.

Warianty tytułu

EN

Simultaneous determination of fat-soluble vitamins by means of HPLC

• Języki publikacji: PL

Abstrakty

EN

In this review, selected chemical techniques such as TLC, UV-VIS, GC, HPLC and microbiological procedures (published since 1960) used for simultaneous determination of fat-soluble vitamins in different matrices have been discussed. Particular attention has been given to HPLC methods, not only in terms of chromatographic conditions, but also in comparison with other separation techniques. HPLC methods have been shown to have clear advantages over existing chemical or microbiological assays in terms of sensitivity, specificity and sample throughput for the analysis of these vitamins. In order to assist the analyst in the selection of a particular procedure, certain chromatographic conditions have been listed for this purpose. However, as currently, no specific HPLC procedures can be recommended, it was decided to develop new methods for these vitamins. Within the past 30 years, HPLC has become the predominant method for separation and quantification of fat-soluble vitamins. The two major, distinct methods of HPLC separation are referred to as normal-phase chromatography and reverse-phase chromatography. To generalise, reverse-phase HPLC is frequently preferred for analysis of biological samples as the columns are more easily purged of any contaminants and sample separation is usually less sensitive to slight changes in mobile-phase composition. A summary of HPLC systems used for fat-soluble vitamins analysis is provided in Table 1 [60-106] and 2 [107-152] in format: compounds determined, matrix, sample preparation and clean up, column, mobile phase, detection, time of analysis, reference. Finally, this paper describes a procedure for the simultaneous determination of vitamin A (retinol acetate, palmitate), vitamin D3 (cholecalciferol), vitamin E (alpha-tocopherol acetate) and alphacalcidol in capsules from a single sample extract using reverse phase HPLC and column backflushing techniques [162-165]. The procedure eliminates saponification, lengthy extractions and sample workup. It is specific to the compounds of interest, shows very good internal precision and is free of interference compared to current published methods.

Słowa kluczowe

PL

witaminy rozpuszczalne w tłuszczach; metoda oznaczania witamin; chromatografia cieczowa

EN

HPLC method; liquid chromatography; fat-soluble vitamins

• Wydawca: Polskie Towarzystwo Chemiczne
• Czasopismo: Wiadomości Chemiczne
• Rocznik: 2001
• Tom: [Z] 55, 11-12
• Strony: 1075--1102
• Opis fizyczny: tab., bibliogr. 165 poz.

Twórcy

autor

Wieliński S.

• Instytut Technologii i Inżynierii Chemicznej, Politechnika Poznańska pl. M. Skłodowskiej-Curie 2, 60-965 Poznań

autor

Olszanowski A.

• Instytut Technologii i Inżynierii Chemicznej, Politechnika Poznańska pl. M. Skłodowskiej-Curie 2, 60-965 Poznań

• Instytut Technologii i Inżynierii Chemicznej, Politechnika Poznańska, pl. M.Skłodowskiej-Curie 2, 60-965 Poznań

Bibliografia

• [1] W. Swiętoslawski, Witaminy i hormony, Warszawa 1950.
• [2] Przewodnik farmakoterapii, Warszawa 1995.
• [3] E. Pawelczyk, Chemia leków, Warszawa 1978.
• [4] USP DI - Drug Information for Health Care Professional, USP XXIII, 1995.
• [5] FPV Farmakopea polska, 1995.
• [6] Dictionary of Drugs, Chemical Data, Structures and Bibliographies, New York 1999.
• [7] Therapeutic Drugs, Toronto 1999.
• [8] ABPI Compendium of Data Sheets and Summaries of Product Characterise, 1998-99.
• [9] AHFS Drugs Information, London 1997.
• [10] Drug Facts and Comparisons, St. Louis 1998.
• [11] Clarkes Isolation and Identification of Drugs, London 1986.
• [12] The Merck Index, 12th ed., New York 1996.
• [13] Lange’s Handbook of Chemistry, New York, 13 ed., 1998.
• [14] Organic-Chemical Drugs and Weir Synonymous, 7th ed.. Akademische Verlag, 1998.
• [15] IUPAC, Commission on the Nomenclature of Biological Chemistry. J. Am Chem Soc 1960 82,5581.
• [16] The United States Pharmacopoeia, USP XXIIÏ. 1995.
• [17] British Pharmacopoeia Data Crown Co, Index System Simulation Ltd, 1998.
• [18] N.T. Gridgeman, Chem. Ind., 1947, 574.
• [19] R.W. Little, Ind. Eng. Chem. (Anal), 1944, 16, 288.
• [20] K.V. John, H.R. Lakshmanan, RB. Jungwala, H.R.Cama, J. Chromatogr., 1965,18, 53.
• [21] T.N.R. Varma, T. Panalaks, T.K. Murray, Anal. Chem., 1964, 36, 1864.
• [22] Y.K. Fung, R.G. Rahwan, J. Chromatogr., 1978,147, 528.
• [23] I.D. Desai, [w:] Vitamin E-A Comprehensive Treatise, Marcel Dekker, New York 1980, s. 67.
• [24] C. Vermeer, N. Comp. Biochem., 1985, 13, 87.
• [25] M.F. Lefevere, A.E. Claeyes, A.P. De Leenheer, Modem Chromatographic Analysis of Vitamins, Marcel Dekker, New York 1985.
• [26] H.K. Lichtenthaler, Handbook o f Chromatography: Lipids, Vol. IL CRS Press. Boca Raton. FI. 1984.
• [27] A.P. De Leenheer, W.E. Lambert, Handbook o f Thin Layer Chromatography, Marcel Dekker, New York 1990, s. 993.
• [28] M.D. Collins, H.N. Shah, J. Appl. Bacteriol., 1980,48, 227.
• [29] Y. Usui, N. Nishimura, N. Kobayashi, T. Okanoue, J. Chromatogr., 1989,489, 291.
• [30] J.W.C. Peereboom, J.B. Roos, J. Chromatogr., 1961, 5. 500.
• [31] P.E. Dunagin, J.A. Olson, Methods Enzymol., 1969, 15, 289.
• [32] T. Ninomiya, K. Kidokoro, M. Horiguchi, Vitamin, 1969, 27, 349.
• [33] J.L. Napoli, Methods Enzymol., 1986, 123, 112.
• [34] G. Arroyave, C.O. Chichester, H. Flores, Biochemical Methodology for the Assessment of Vitamin A Status, International Vitamin A Consultative Group, ILSI-NF, Washington DC 1982.
• [35] D. Sklan, P. Budowski, M. Katz, Anal. Biochem., 1978, 56, 606.
• [36] D.A. Seamark, D.J.H. Trafford, H.L. Makin, J. Steroid Biochem., 1980,13. 1057.
• [37] H. Ziffer, W.J.A. Van den Heuvel, E.O.A. Haathi, J. Am. Chem. Soc., 1960, 82, 6411.
• [38] G.H. Dialameh, R.E. Olson, Anal. Biochem., 1969, 32, 262.
• [39] P.W. Meijboom, G.A. Jongenotter, J. Aim. Oil Chem. Soc., 1979, 56, 33.
• [40] A. Kormann, J. Lipid Res., 1980, 21, 780.
• [41] S. Ishikawa, G. Katsui, J. Vitaminol., 1966,12,106.
• [42] Y. Usui, N. Nishimura, N. Kobayashi, T. Okanoue, J. Chromatogr., 1989, 489, 291.
• [43] A.J. Sheppard, A.R. Prosser, J. Am. Oil Chem. Soc., 1972, 49, 619.
• [44] M.F. Lefevere, R.W. Frei, Chromatographia, 1982,15, 459.
• [45] F.H. Carr, E.A. Price, Biochem J., 1926, 20, 497.
• [46] P. Budowski, A. Bondi, Analyst, 1957, 82, 791.
• [47] A.E. Sobel, W. Werbin, Ind. Eng. Chem., 1946,18, 570.
• [48] M. Brochmann, Y.H. Chenn, Z. Physiol. Chem., 1936, 241, 129.
• [49] C.A. Frolik, J.A. Olson, The Retinoids, Vol. 1, Academic Press, New York 1984, s. 181.
• [50] A.J. Clifford, A.D. Jones, Methods Enzymol.. 1991, 189, 94.
• [51] M. Furter, R.E. Meyer, Helv. Chem. Acta, 1939, 22, 240.
• [52] M. Kofler, ibid., 1942, 25, 1469.
• [53] Y. Sahashi, T. Suzuki, M. Higaki, J. Vitaminol., 1967, 13, 33.
• [54] D.R. Lakdawala, E.M. Widdowson, Lancet, 1977,1, 167.
• [55] E. Leerbeck, H. Sondergaard, Br. J. Nutr., 1980, 44, 7.
• [56] M.L. Ribovich, H.F. Deluca, Arch. Biochem. Biophys., 1978,188, 145.
• [57] R.E. Majors, LC-GC, 1991, 4, 2, 10
• [58] J. Namieśnik, J. Łukasiak, Z. Jamrógiewicz, Pobieranie próbek do analizy, PWN, Warszawa 1995.
• [59] J. Namieśnik, J. Łukasiak, Z. Jamrógiewicz, Podstawy analityki, Akademia Medyczna w Gdańsku, Gdańsk 1992.
• [60] T. Hofsass, Symp. Proc. Assoc. Vitamin Chem., Chicago, 1978, 1.
• [61] D.K. Feeter, M.F. Jacobs, H.W. Rawlings, J. Pharm. Sci., 1971, 60, 913.
• [62] Von Syed Laik Ali, D. Steinbach, Pharmaz. Ztg. 1980,125, 2079.
• [63] K. Aitzetmuller, Fat Sci. Technol., 1994, 8, 279.
• [64] E. Brinkmann, 1. Mehlitz, H. Bijosono Oei, R. Tiebach, W. Baltes, Z. Lebens. Unters. Forsch., 1994,199, 206.
• [65] G. Jones, K. Kano, S. Yamada, T. Furusawa, H. Takayama, T. Suda, Biochemistry, 1984,23, 3749.
• [66] G. Jones, M. Kung, K. Kano, J. Biol. Chem., 1983, 258, 12920.
• [67] AJ. Speek, J. Schrijver, W.P. Schreurs, J. Food Sci., 1985, 50, 121.
• [68] K. Albert, H. Handel, M. Pursch, S. Strohschein, Chemically Modified Surfaces, Spec. Publ. Royal Soc. Chem, 1996, 173, 30.
• [69] D. Blitek, N. Sadlej-Sosnowska, I. Wilczyńska-Wojtulewicz, Acta Polon. Pharm. XLIII, 1986,5,465.
• [70] G.M. Landers, J.A. Olson, J. Assoc. Off. Anal. Chem., 1986,1, 50.
• [71] R.G. Pozo, E.S. Saitua, I. Uncilla, J.A. Montoya, J. Food Science, 1990, 1, 77.
• [72] Y. Takashima, T. Nakajima, S. Tanaka, M. Washitake, T. Anmo, H. Matsumaru, Chem. Pharm. Bull., 1979, 7, 1553.
• [73] M.I.R. Miritello Santoro, P.M.B.G. Maia Campos, E.R.M. Hackmann, Cosmetics & Toiletries, 1993,108, 71.
• [74] B. Stancher, F. Żonata, J. Chromatogr., 1984, 312, 423.
• [75] F. Żonata, B. Stacher, ibid., 1984, 301, 65.
• [76] K.W. Miller, Ch.S. Yang, Anal. Biochem., 1985, 145, 21.
• [77] A.A. Moustafa, E.M. Abdel-Moety, S.A. Wise, Grasas Acietes, 1987, 4, 224.
• [78] G. Jones, H.F. DeLuca, J. Lipid Research, 1975, 16, 448.
• [79] M.F. Holick, H.K. Schnoes, H.F. DeLuca, Proc. Nat. Acad. Sci. USA, 1971, 4, 803.
• [80] A.T. Rhys Williams, J. Chromatogr., 1985, 341, 198.
• [81] B.W. Hollis, N.E. Frank, ibid, 1985, 343, 43.
• [82] J.S. Adams, T.L. Clemens, M.F. Holick, ibid, 1981, 226, 198.
• [83] G. Jones, ibid, 1980, 221, 27.
• [84] R. Belsey, H.F. DeLuca, J.T. Potts, J. Clin. Endocrinol. Metab., 1971, 33, 554.
• [85] N.R. Badcock, D.A. O’Reilly, J. Chromatogr., 1986, 382, 290.
• [86] M.G. De Ruyter, W.E. Lambert, A.P. De Leenheer, Anal. Biochem., 1979, 98, 402.
• [87] S. Giys, Metody rozdziału w analizie medycznej i farmaceutycznej, Łódź 1999.
• [88] G.A. Walker, B.E. Carpenter, D.L. Tuescher, J. Pharm. Sciences, 1980, 7, 846.
• [89] B.L. Lee, S.C. Chua, H.Y. Ong, C.N. Ong, J. Chromatogr., 1992, 581, 41.
• [90] M. Mulholland, R.J. Dolphin, ibid., 1985, 350, 285.
• [91] U. Manz, Beta-Carotene Assay Methods, Blood Plasma and Milk, 1980, s. 1743.
• [92] U. Mantz, Pigmenting Carotenoids - Analytical Methods, Premixes, 1983, s. 1864.
• [93] H.E. Keller, Analytical Methods for Determination of Vitamins in Multivitamin Preparations, Roche Basle 1984, s. 1887.
• [94] H.E. Keller, Analytical Methods for Vitamins and Carotenoids in Feed, Roche Basle 1995.
• [95] M. Mulholland, R.J. Dolphin, J. Chromatogr., 1985, 350, 285.
• [96] M. Mulholland, Analyst, 1986, 111, 601.
• [97] R. Mansourian, E. Shepherd, H. Dirren, Int. J. Vit. Nutr. Res., 1982, 52, 227.
• [98] D.C. Woollard, H. Indyk, J. Micronutr. Anal., 1989, 5, 35.
• [99] B. Tan, L. Brzuskiewicz, Anal. Biochem., 1989, 180, 368.
• [100] A J. Speek, J. Schrijver, W.P. Schreurs, J. Food Sci., 1985, 50, 121.
• [101] D.T. Bums, C. Mackay, J. Chromatogr., 1980, 200, 300.
• [102] P. Taylor, P. Barnes, Chem. Ind., 1981, 20, 722.
• [103] A. Pitersma-de Bruyn, P. van Haard, Clin. Chim. Acta, 1985,150, 95.
• [104] A.C. Wilson, B.K. Park. J. Chromatogr., 1983, 227, 292.
• [105] K. Hirauchi, T. Sakano, A. Morimoto, Chem. Pharm. Bull., 1986, 34, 845.
• [106] L.L. Mummah-Schendel, J.W. Suttie, Am. J. Clin. Nutr., 1986, 44, 686.
• [107] A. Celardo, A . Bortolotti, J. Chromatogr., 1989, 490, 432.
• [108] W.A. MacCrehan, E. Schonberger, J. Chromatogr. B, 1995. 670, 209.
• [109] S.L. Reynolds, H.J. Judd, Analyst, 1984,109,489.
• [110] S. Kamel, M. Brazier, C. Picard, F. Boitte, L. Samson, G. Desmet, J.L. Sebert, Bone & Mineral, 1994. 26, 197.
• [111] H. Johnsson, H. Hessel, Internat. J. Vit. Nutr. Res., 1987, 57, 357.
• [112] E. Jakob, I. Elmadfa, ibid., 1995, 65, 31.
• [113] E. Jakob, I. Elmadfa, Food Chemistry, 1996, 1, 87.
• [114] W.W. Borland, A. Shenkin, Ann. Clin. Biochem., 1987, 24, 111.
• [115] M. Amin, J. Liq. Chromatogr., 1987, 14, 3127.
• [116] M. Amin, ibid., 1988, 6, 1335.
• [117] M. Amin, ibid., 1988, 6, 1347.
• [118] C. Gensestar. F. Grases, Chromatographia, 1995, 3/4, 143.
• [119] M. Mulholland, Analyst, 1986,111, 601.
• [120] Y. Arai, T. Hanai, J. Liq. Chromatogr., 1988, 11, 2409.
• [121] R. Andreoli, M. Careri, P. Manini, G. Mori, M. Musci, Chromatographia, 1997,11/12. 605.
• [122] B. Yeung, P. Vouros, G. Reddy, Am. Lab., 1994, 26, 12.
• [123] S.A. Bamett, L.W. Frick, Anal. Chem., 1979, 6, 641.
• [124] P.A. Jackson, Food Flavour Ingred. Packag. Process, 1982, 4, 12.
• [125] M. Mulholland, R.J. Dolphin, J. Chromatogr., 1985, 350, 285.
• [126] H.C. Furr, D.A. Cooper, J.A. Olson, ibid.. 1986, 378, 45.
• [127] L. Yakushina, A. Taranova, J. Pharm. Biomed. Anal., 1995, 4/5, 715.
• [128] S. Scalia, G. Ruberto, F. Bonina, J. Pharm. Sei., 1995, 4, 433.
• [129] W.T. Wahyuni, K. Jinno, J. Micronutr. Anal., 1987,3, 47.
• [130] W.J. Driskell, J.W. Nesse, C.C. Bryant, M.M. Bashor, J. Chromatogr., 1982. 231, 439.
• [131] S. Scalia, A Renda, G. Ruberto, F. Bonina, E. Menegattí, J. Pharm. Biomed. Anal., 1995,3, 273.
• [132] A.C. Ross, Anal. Biochem., 1981, 115, 324.
• [133] V. Fernandez, H.M. Abdou, F.M. Russo-Alesi, Pharm. Technol., 1981, 5, 40.
• [134] G Jones, Clin. Chem., 1978, 2, 287.
• [135] M.J. Russell, B.S. Thomas, E. Wellock, J. High Resolution Chrom. & Chrom. Communications, 1986, 9, 281.
• [136] C.A. Collins, C.H.K. Chow, J. Chromatogr., 1984, 317, 349.
• [137] W.A. MacCrehan, E. Schonberger, Clin. Chem., 1987, 33, 1585.
• [138] H. Turnbull, D.J.H. Trafford, H.L.J. Makin, Clin. Chim. Acta, 1982, 120, 65.
• [139] H.J. Nelis. J. DeRoose, H. Vandenbaviere, A.P. DeLeenheer, Clin. Chem., 1983, 29, 1431.
• [140] J.C. Wallingford, B.A. Underwood, J. Chromatogr., 1986, 381, 158.
• [141] A.C. Ross, Methods Enzymol., 1986,123, 68.
• [142] C.J.L. Bugge, L.C. Rodriquez, F.M. Vane, J. Pharm. Biomed. Anal., 1985, 3, 269.
• [143] W.A. MacCrehan, E. Schonberger, Clin., Chem., 1987,33, 1585.
• [144] G.L. Catignani, J.G. Bieri, ibid. 1983, 29, 708.
• [145] H.E. May, S.I. Koo, J. Liq. Chromatogr., 1989,12, 1261.
• [146] H.C. Furr, D.A. Cooper, J.A. Olson, J. Chromatogr., 1986, 378,45.
• [147] A.B. Barua, Methods Enzymol., 1991,189, 136.
• [148] E.W. Matthews, P.G.H. Byfield, K.W. Colston, I.M.A. Evans, L.S. Galante, I. Maclntryre, FEBS Lett., 1974, 48, 122.
• [149] G. Jones, J. Chromatogr. Biomed. Applic., 1980, 221, 27.
• [150] G. Makin, D. Lohnes, V. Byford, R. Ray, G. Jones, Biochem. J., 1989, 262, 173.
• [151] G. Jones, K. Kano, S. Yamada, T. Furusawa, H. Takayama, T. Suda, Biochemistry, 1984, 23, 3749.
• [152] G. Jones, N. Edwards, D. Vriezen, C. Porteous, D.J.H. Trafford, J. Cunningham, H.L.J. Makin, ibid., 1988, 27, 7070.
• [153] R.D. Coldwell, C.E. Porteous, D.J.H. Trafford, H.L.J Makin, Steroids, 1987, 49, 155.
• [154] A.M. Rosenthal, G. Jones, S.W. Kooh, D. Fraser, Am. J. Physiol., 1980, 239, E 12.
• [155] G. Jones, K. Kano, Biochemistry, 1985, 25, 4493.
• [156] G.L. Catignani, J.G. Bieri, Clin. Chem., 1983, 27, 471.
• [157] H.E. Indyk, Analyst., 1988,113, 1217.
• [158] C.S.J. Shen, A.J. Sheppard, J. Micronutr. Anal., 1986, 2, 43.
• [159] T. Ueno, J.W. Suttie, Anal. Biochem., 1983, 133, 62.
• [160] AOAC Official Methods o f Analysis, 1990.
• [161] J.A. Olson, [w:] Handbook of Vitamins, L. Machlin, (red.), Marcel Dekker, New York 1990.
• [162] S. Wieliński, A. Olszanowski, Biuletyn Instytutu Leków, 1998, 1,2, 18.
• [163] S. Wieliński, A. Olszanowski, J. Liq. Chrom. & Rel. Technol., 1999, 22, 3115.
• [164] S. Wieliński, A. Olszanowski, Chromatographia, 1999, 50, 109.
• [165] S. Wieliński, A. Olszanowski, J. Liq. Chrom. & Rel. Technol., 2001, 24, 61.

Uwagi

PL

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