Preparation, quality control and biodistribution studies of [61Cu]-oxinate for PET tumor imaging

• Autorzy: Jalilian A. R. , Zolghadri S. , Faghihi R. , Yousefnia H. , Garousi J. , Shafaii K. , Bolourinovin F.
• Języki publikacji: EN

Abstrakty

EN

Targeting apoptosis is an interesting issue in molecular imaging and various modalities have been presented. However, recent experiences in nuclear pharmacy demonstrated the application of small tracer molecules is more desired. This work was conducted for production of a radiolabeled copper complex, i.e. 61Cu-oxinate as a potential PET tracer for apoptosis imaging in oncology. Cu-61 was prepared by natural zinc target irradiation with 22 MeV protons (150 miA) via the natZn(p, xn)61Cu nuclear reaction with a yield of 3.33 mCi/miAh. In order to obtain the best labeling method, optimization reactions were performed for pH, temperature and concentration followed by solid phase extraction. Biodistribution of the tracer was studied in wild-type and fibrosarcoma bearing mice. Under the optimized conditions, radio-thin-layer chromatography (RTLC) and HPLC showed radiochemical purities of 99.99% and 97% respectively (with a minimum specific activity of 16 Ci/mM). Biodistribution of the tracer in fibrosarcoma bearing mice demonstrated a significant tumor uptake after 3 h. Tumor:blood and tumor:muscle ratios were 2.0 and 6.0 after 3 h, respectively.

Słowa kluczowe

EN

copper-61; 8-hydroxyquinoline; positron emission tomography; fibrosarcoma

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2009
• Tom: Vol. 54, No. 3
• Strony: 175--179
• Opis fizyczny: Bibliogr. 15 poz., rys.

Twórcy

autor

Jalilian A. R.

autor

Zolghadri S.

autor

Faghihi R.

autor

Yousefnia H.

autor

Garousi J.

autor

Shafaii K.

autor

Bolourinovin F.

• Agricultural, Medical and Industrial Research School (AMIRS-NSTRI), Karaj, Iran, P. O. Box 31485-498, Tel./Fax: [phone],

Bibliografia

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