Application of Enzyme Labeled Antibodies for Immunosensors Based on Electrochem ical Detection

• Autorzy: Kazimierczak B. , Pijanowska D. , Torbicz W.
• Konferencja: Symposium "From Molecular Recognition to Molecular Devices", September 20-22, Gdańsk, Poland
• Języki publikacji: EN

Abstrakty

EN

In this paper, immunosensors based on electrochemical (potentiometric and amperometric) detection are presented. We developed ISFET type potentiometric immunosensor with urease as a label and a screen-printed amperometric immunosensor with alkaline phosphatase, utilizing ascorbic acid phosphate as a substrate. For the free alkaline phosphatase in concentration range 35-3500 pM, the response of amperometric sensors was at the level from 10 to 80 miA. It was stated that the potentiometric immunosensors can be applied for measurements of the to tal gamma-globulin concentration or for detection of gamma the selected IgG in concentration range 1.37 mg/L-4 g/L.

Słowa kluczowe

EN

immunosensor; urease; alkaline phosphatase; ascorbic acid phosphate; potentiometric detection; amperometric detection

• Wydawca: Polskie Towarzystwo Chemiczne
• Czasopismo: Polish Journal of Chemistry
• Rocznik: 2008
• Tom: Vol. 82, nr 6
• Strony: 1255--1263
• Opis fizyczny: Bibliogr. 11 poz., rys.

Twórcy

autor

Kazimierczak B.

autor

Pijanowska D.

autor

Torbicz W.

• Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, ul. Ks. Trojdena 4, 02-109 Warsaw, Poland,

Bibliografia

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