Preparation and biodistribution of [67Ga]-insulin for SPECT purposes

• Autorzy: Jalilian A. R. , Garousi J. , Gholami E. , Akhlaghi M. , Bolourinovin , Rajabifar S.
• Języki publikacji: EN

Abstrakty

EN

Human recombinant insulin was successively labeled with [67Ga]-gallium chloride after conjugation with freshly prepared cyclic DTPA-dianhydride (ccDTPA). The best results of the conjugation were obtained by the addition of 0.5 ml of an insulin pharmaceutical solution (5 mg/ml, in phosphate buffer, pH = 8) to a glass tube precoated with DTPA-dianhydride (0.01 mg) at 25°C with continuous mild stirring for 30 min. Radiothin-layer chromatography (RTLC), instant thin-layer chromatography (ITLC) and high-performance liquid chromatography (HPLC) showed overall radiochemical purity higher than 96% in optimized conditions (specific activity = 300 500 MBq/mg, labeling efficiency 77%). Preliminary in vivo studies with normal rats were performed to determine the biodistribution of the radiotracer up to 110 h. They showed a high liver uptake of the tracer which is consistent with other reported radiolabeled insulins.

Słowa kluczowe

EN

radiogallium; insulin; radiolabeling; biodistribution; radiopharmaceutical; cyclotron

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2007
• Tom: Vol. 52, nr 4
• Strony: 145--151
• Opis fizyczny: Bibliogr. 24 poz., rys.

Twórcy

autor

Jalilian A. R.

autor

Garousi J.

autor

Gholami E.

autor

Akhlaghi M.

autor

Bolourinovin

autor

Rajabifar S.

• Nuclear Medicine Group, Agriculture, Medicine and Industrial Research School (AMIRS-NSTRI), P. O. Box 31485-498, Karaj, Iran,

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