Modulation of the growth of pulmonary tumour colonies in mice after single or fractionated low-level irradiations with X-rays

• Autorzy: Nowosielska E. M. , Cheda A. , Wrembel-Wargocka J. , Janiak M. K.
• Konferencja: Proceedings of the 14th National Scientific Meeting of the Polish Radiation Society 24-26 Septemebr 2007, Kielce, Poland
• Języki publikacji: EN

Abstrakty

EN

A number of epidemiological and experimental data indicate that exposures to low doses of low-LET ionizing radiation may trigger the activity of natural anti-tumour immune mechanisms and inhibit tumour growth. Natural killer (NK) cells and activated macrophages play important roles in the anti-tumour defence of the host. In view of this, the aim of the present study was to correlate the tumour-inhibitory effect of low doses of X-rays with the activities of NK cells and macrophages. BALB/c mice were whole-body irradiated with single or fractionated doses of 0.1, 0.2, or 1.0 Gy X-rays and then intravenously injected with L1 sarcoma cells; 14 days later, tumour colonies on the lungs' surface were counted. Cytotoxic activities of NK cells and macrophages were estimated using the 51Cr-release and [3H]thymidineuptake assays, respectively. The anti-asialo GM1 antibody and carrageenan (CGN) were intraperitoneally injected to block the NK cell- and macrophage-mediated activities in vivo, respectively. Single and fractionated whole-body irradiation (WBI) of mice with 0.1 or 0.2 Gy X-rays led to reduction in the number of the pulmonary tumour colonies accompanied by the enhanced cytotoxic activities of both NK lymphocytes and macrophages. Treatment of mice with anti-asialo GM1 antibody or CGN abrogated the tumour-inhibitory effects of the low-level exposures to X-rays. The obtained data suggest that suppression of the development of pulmonary tumour colonies by single or fractionated irradiations of mice with the low doses of X-rays may result from stimulation of the natural anti-tumour defence reactions mediated by NK cells and/or cytotoxic macrophages.

Słowa kluczowe

EN

low-level X-rays; NK cells; macrophages; tumour colonies; anti-neoplastic activity

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2008
• Tom: Vol. 53, suppl. 1
• Strony: 9--15
• Opis fizyczny: Bibliogr. 53 poz., rys.

Twórcy

autor

Nowosielska E. M.

autor

Cheda A.

autor

Wrembel-Wargocka J.

autor

Janiak M. K.

• Military Institute of Hygiene and Epidemiology, Department of Radiobiology and Radiation Protection, 4 Kozielska Str., 01-163 Warsaw, Poland, Tel.: [phone], Fax: [phone],

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