Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
Tytuł artykułu

Modulation of the growth of pulmonary tumour colonies in mice after single or fractionated low-level irradiations with X-rays

Treść / Zawartość
Warianty tytułu
Proceedings of the 14th National Scientific Meeting of the Polish Radiation Society 24-26 Septemebr 2007, Kielce, Poland
Języki publikacji
A number of epidemiological and experimental data indicate that exposures to low doses of low-LET ionizing radiation may trigger the activity of natural anti-tumour immune mechanisms and inhibit tumour growth. Natural killer (NK) cells and activated macrophages play important roles in the anti-tumour defence of the host. In view of this, the aim of the present study was to correlate the tumour-inhibitory effect of low doses of X-rays with the activities of NK cells and macrophages. BALB/c mice were whole-body irradiated with single or fractionated doses of 0.1, 0.2, or 1.0 Gy X-rays and then intravenously injected with L1 sarcoma cells; 14 days later, tumour colonies on the lungs' surface were counted. Cytotoxic activities of NK cells and macrophages were estimated using the 51Cr-release and [3H]thymidineuptake assays, respectively. The anti-asialo GM1 antibody and carrageenan (CGN) were intraperitoneally injected to block the NK cell- and macrophage-mediated activities in vivo, respectively. Single and fractionated whole-body irradiation (WBI) of mice with 0.1 or 0.2 Gy X-rays led to reduction in the number of the pulmonary tumour colonies accompanied by the enhanced cytotoxic activities of both NK lymphocytes and macrophages. Treatment of mice with anti-asialo GM1 antibody or CGN abrogated the tumour-inhibitory effects of the low-level exposures to X-rays. The obtained data suggest that suppression of the development of pulmonary tumour colonies by single or fractionated irradiations of mice with the low doses of X-rays may result from stimulation of the natural anti-tumour defence reactions mediated by NK cells and/or cytotoxic macrophages.
Opis fizyczny
Bibliogr. 53 poz., rys.
  • Military Institute of Hygiene and Epidemiology, Department of Radiobiology and Radiation Protection, 4 Kozielska Str., 01-163 Warsaw, Poland, Tel.: +48 22 681 6135, Fax: +48 22 810 4391,
  • 1. Al-Sarireh B, Eremin O (2000) Tumour-associated macrophages (TAMS): disordered function, immune suppression and progressive tumour growth. J R Coll Surg Edinb 45:1–16
  • 2. Anderson RE (1982) Radiation-induced augmentation of response of A/J mice to SaI tumor cells. Am J Pathol 108:24–37
  • 3. Barao I, Ascensao JL (1998) Human natural killer cells. Arch Immunol Ther Exp 46:213–229
  • 4. Berrington A, Darby SC, Weiss HA, Doll R (2001) 100 years of observation on British radiologists: mortality from cancer and other causes 1897–1997. Br J Radiol 74:507–519
  • 5. Cai L (1999) Research of the adaptive response induced by low-dose radiation: where have we been and where should we go? Hum Exp Toxicol 18:419–425
  • 6. Caratero A, Courtade M, Bonnet L, Planel H, Caratero C (1998) Effect of a continuous gamma irradiation at a very low dose on the life span of mice. Gerontology 44:272–276
  • 7. Cardis E, Gilbert ES, Carpenter L et al. (1995) Effects of low doses and low dose rates of external ionizing radiation:cancer mortality among nuclear industry workers in three countries. Radiat Res 142:117–132
  • 8. Chen WL, Luan YC, Shieh MC et al. (2004) Is chronic radiation an effective prophylaxis against cancer? J Am Physicians Surg 9:6–10
  • 9. Cohen BL (1995) Test the linear-no threshold theory of radiation carcinogenesis for inhaled radon decay products. Health Phys 86:157–174
  • 10. Cohen BL (1997) Problems in the radon vs. lung cancer test of linear no-threshold theory and a procedure for resolving them. Health Phys 72:623–628
  • 11. Farias-Eisner R, Sherman MP, Aeberhard E, Chaudhuri G (1994) Nitric oxide is an important mediator for tumoricidal activity in vivo. Proc Natl Acad Sci USA 91:9407–9411
  • 12. Harrington NP, Chambers KA, Ross WM, Filion LG (1997) Radiation damage and immune suppression in splenic mononuclear cell populations. Clin Exp Immunol 107:417–424
  • 13. Hashimoto S, Shirato H, Hosokawa M et al. (1999) The suppression of metastases and the change in host immune response after low-dose total-body irradiation in tumorbearing rats. Radiat Res 151:717–724
  • 14. Hosoi Y, Sakamoto K (1993) Suppressive effect of low dose total body irradiation on lung metastasis: dose dependency and effective period. Radiother Oncol 26:177–179
  • 15. Ibuki Y, Goto R (1995) Augmentation of NO production and cytolytic activity of Mφ obtained from mice irradiated with a low dose of gamma-rays. J Radiat Res 36:209–220
  • 16. Ibuki Y, Goto R (1997) Enhancement of NO production from resident peritoneal macrophages by in vitro gamma-irradiation and its relationship to reactive oxygen intermediates. Free Radic Biol Med 22:1029–1035
  • 17. Ina Y, Sakai K (2004) Prolongation of life span associated with immunological modification by chronic lowdose-rate irradiation in MRL-lpr/lpr mice. Radiat Res 16:168–173
  • 18. Ina Y, Sakai K (2005) Activation of immunological network by chronic low-dose-rate irradiation in wild-type mouse strains; analysis of immune cell populations surface molecules. Int J Radiat Biol 81:721–729
  • 19. Ishii K, Hosoi Y, Yamada S, Ono T, Sakamoto K (1996) Decreased incidence of thymic lymphoma in AKR mice as a result of chronic, fractionated low-dose total-body X irradiation. Radiat Res 146:582–585
  • 20. Jagger J (1998) Natural background radiation and cancer death in Rocky Mountain states and Gulf Coast states.Health Phys 7:428–430
  • 21. Ju GZ, Liu SZ, Li XY, Liu WH, Fu HQ (1995) Effect of high vs. low dose radiation on the immune system. In:Hagen U, Harder D, Jung H, Streffer C (eds) Radiation research 1895–1995. Proc of the 10th Int Congress of Radiation Research, 27 August – 1 September 1995, Würzburg, Germany. IARR, Würzburg, pp 709–714
  • 22. Katayama H, Matsuura M, Enndo S, Hosoi M, Othaki M, Hayakawa N (2002) Reassessment of the cancer mortality risk among Hiroshima atomic-bomb survivors using a new dosimetry system, ABS2000D, compared with ABS93D. J Radiat Res 43:53–64
  • 23. Kesavan PC (1997) Indian research on high levels of natural radiation: pertinent observations for further studies. In: Wei L, Sugahara T, Tao Z (eds) High levels of natural radiation, 1996: radiation dose and health effects. Proc of the 4th Int Conf on High Levels of Natural Radiation, 21–25 October 1996, Beijing, China. Elsevier, Amsterdam, pp 112–117
  • 24. Kojima S, Ishida H, Takahashi M, Yamaoka K (2002) Elevation of glutathione induced by low-dose gamma rays and its involvement in increased natural killer activity. Radiat Res 157:275–280
  • 25. Kojima S, Nakayama K, Ishida H (2004) Low dose gamma-rays activate immune functions via induction of glutathione and delay tumor growth. J Radiat Res 45:33–39
  • 26. Kondo S (1993) Health effects of low level radiation. Kinki University Press, Osaka, Japan; Medical Physics Publishing, Madison, WI, USA
  • 27. Lin IH, Hau DM, Chen WC, Chen KT (1996) Effects of low dose gamma-ray irradiation on peripheral leukocyte counts and spleen of mice. Chin Med J 109:210–214
  • 28. Little MP, Weiss HA, Boice JD (1999) Risks of leukemia in Japanese atomic bomb survivors, in women treated for cervical cancer, and in patients treated for ankylosing spondylitis. Radiat Res 152:280–292
  • 29. Liu SZ (2004) Cancer control related to stimulation of immunity by low dose radiation. In: Proc of the 14th Pacific Basin Nuclear Conference, 21–25 March 2004, Honolulu, USA. American Nuclear Society, La Grange Park, Illinois, pp 368–372
  • 30. Liu SZ, Han ZB, Liu WH (1994) Changes in lymphocyte reactivity to modulatory factors following low dose ionizing radiation. Biomed Environ Sci 7:130–135
  • 31. Liu SZ, Su X, Zhang YC, Zhao Y (1994) Signal transduction in lymphocytes after low dose radiation. Chin Med J 107:431–436
  • 32. Liu XD, Ma SM, Liu SZ (2003) Effects of 0.075 Gy X-ray irradiation on the expression of IL-10 and IL-12 in mice. Phys Med Biol 48:2041–2049
  • 33. Luckey TD (1999) Nurture with ionising radiation: a provocative hypothesis. Nutr Cancer 34:1–11
  • 34. McKinney LC, Aquilla EM, Coffin D, Wink DA, Vodovotz Y (1998) Origin and functions of human natural killer cells. J Leukocyte Biol 64:459–466
  • 35. Matanoski GM, Santos-Burgoa C, Schwartz L (1990) Mortality of a cohort of workers in the styrene-butadiene polymer manufacturing industry (1943–1982). Environ Health Perspect 86:107–17
  • 36. Minarovits J, Karczag E, Földes I (1989) Enhanced take of spontaneous murine tumors in mice treated with inhibitors of macrophage and/or NK cell function. Neoplasma 36:3–9
  • 37. Mitchel RE, Jackson JS, McCann RA, Boreham DR (1999) The adaptive response modifies latency for radiation-induced myeloid leukemia in CBAH mice.Radiat Res 152:273–279
  • 38. Mitchel RE, Jackson JS, Morrison DP, Carlisle SM (2003) Low doses of radiation increase the latency of spontaneous lymphomas and spinal osteosarcomas in cancer-prone, radiation-sensitive Trp53 heterozygous mice. Radiat Res 159:320–327
  • 39. Moretta L, Ciccone E, Poggi A, Mingari MC, Moretta A (1994) Origin and functions of human natural killer cells. Int J Clin Lab Res 24:181–186
  • 40. Nathan C (1991) Mechanisms and modulation of macrophage activation. Behrings Inst Mitt 88:200–207
  • 41. Pierce DA, Shimizu Y, Preston DL, Vaeth M, Mabuchi K (1996) Studies of the mortality of atomic bomb survivors. Report 12, Part I. Cancer: 1950–1990. Radiat Res 146:1–27
  • 42. Pollycove M (2004) Radiobiological basis of low dose irradiation in prevention and therapy of cancer. In: Proc of the 14th Pacific Basin Nuclear Conference, 21–25 March 2004, Honolulu, USA. American Nuclear Society, La Grange Park, Illinois, pp 647–653
  • 43. Reyburn H, Mandelboim O, Vales-Gomez M et al. (1997) Human NK cells: their ligands, receptors and functions. Immunol Rev 155:119–125
  • 44. Safwat A (2000) The immunology of low-dose total-body irradiation: more questions than answers. Radiat Res 153:599–604
  • 45. Safwat A (2000) The role of low-dose total body irradiation in treatment of non-Hodgkin’s lymphoma: a new look at an old method. Radiother Oncol 56:1–6
  • 46. Safwat A, Aggerholm N, Roit I, Overgaard J, Hokland M (2004) Tumour burden and interleukin-2 dose affect the interaction between low-dose total body irradiation and interleukin 2. Eur J Cancer 40:1412–1417
  • 47. Sakai K, Hosoi Y, Nokamura T, Oda T, Iwasaki T, Yamada T (2003) Suppression of carcinogenic processes in mice by chronic low doses rate gamma-irradiation. Int J Low Radiat 1:142–146
  • 48. UNSCEAR (1986) Genetic and somatic effects of ionizing radiation. Report to the General Assembly, with Annexes. United Nations Publ. E.86.IX, UN, New York
  • 49. UNSCEAR (2006) Sources-to-effects assessment for radon in homes and workplaces. United Nations Scientific Committee on the Effects of Atomic Radiation, Fifty-fourth session, 29 May – 2 June 2006, Vienna.A/AC.82/R.654
  • 50. Wang GJ, Lay L (2000) Induction of cell-proliferation hormesis and cell-survival adaptive response in mouse hematopoietic cells by whole-body low-dose radiation. Toxicol Sci 53:369–376
  • 51. Wang Z, Lubin JH, Wang L et al. (2002) Residential radon and lung cancer risk in a high-exposure area of Gansu Province, China. Am J Epidemiol 155:554–564
  • 52. Wei L, Sugahara T (2000) An introductory overview of the epidemiological study on the population at the high background radiation areas in Yangjiang, China. J Radiat Res 41;Suppl:1–7
  • 53. Young HA, Ortaldo J (2006) Cytokines as critical co-stimulatory molecules in modulating the immune response of natural killer cells. Cell Res 16:20–24
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.