Genotoxic effects of 60Co gamma-rays on Chinese hamster ovary (CHO) cells

• Autorzy: Dicu T. , Brie I. , Virag P. , Fischer E. , Perde M. , Foriş V. , Cernea V. , Cosma C.
• Języki publikacji: EN

Abstrakty

EN

The aim of the present study was to evaluate the cellular radiosensitivity and radiation-induced DNA damage and repair in Chinese hamster ovary (CHO) cells. Cell survival after irradiation was assessed using the clonogenic assay. The initial, radio-induced and residual DNA damage in Chinese hamster ovary (CHO) cells exposed to 60Co gamma-rays were determined using the alkaline comet assay. A linear-quadratic (LQ) survival curve was observed in CHO line. Data obtained by comet assay demonstrated a linear dose-response correlation in the range of tested doses (0.3-4 Gy). The process of DNA repair was modeled by exponential equation. In addition, we found a good correlation (R2 = 0.995) between clonogenic cell survival and radio-induced DNA damage.

Słowa kluczowe

EN

cellular radiosensitivity; genotoxic effects; ionizing radiation; comet assay

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2008
• Tom: Vol. 53, No. 4
• Strony: 161--165
• Opis fizyczny: Bibliogr. 24 poz., rys.

Twórcy

autor

Dicu T.

autor

Brie I.

autor

Virag P.

autor

Fischer E.

autor

Perde M.

autor

Foriş V.

autor

Cernea V.

autor

Cosma C.

• Faculty of Environmental Science, "Babes-Bolyai" University, 4 Ştefan cel Mare, Cluj-Napoca, 400192, Romania, Tel.: [phone]; Fax: [phone],

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