DNA damage in subpopulations of human lymphocytes irradiated with doses in the range of 0-1 Gy of X-radiation

• Autorzy: Wojewódzka M. , Machaj E. K. , Goździk A. , Iwaneńko T. , Ołdak T. , Kruszewski M. , Pojda Z.
• Języki publikacji: EN

Abstrakty

EN

We compared three methods usually applied in biological dosimetry for estimation of radiation-induced DNA damage in human T and B lymphocytes: alkaline comet assay, micronucleus (MN) test and formation of histone gamma-H2AX foci. Human peripheral blood lymphocytes were fractionated using T cells and B cells isolation kits. Cells were irradiated with doses in the range of 0-1 Gy of X-rays. Induction of DNA damage was assessed by the standard alkaline comet assay, MN test and histone gammaH2AX foci immunofluorescence assay. Notwithstanding different end-points measured by the applied methods, all tests revealed a similar induction of DNA damage in B lymphocytes as compared with T lymphocytes. The results indicated that all three tests detect DNA damage with similar sensitivity, the lowest dose being approximately 0.3 Gy. The difference between irradiated and control cells was expressed as the ratio of the value obtained for irradiated cells (1 Gy) to that for control cells. The highest ratio was obtained for formation of gammaH2AX foci and was 6.2 for T and 13.8 for B lymphocytes, whereas those for comet assay and micronucleus test were 3.5; 3.6 and 5.6; 4.8, respectively.

Słowa kluczowe

EN

alkaline comet assay; biodosimetry; micronucleus test; histone gammaH2AX foci immunofluorescence assay; T lymphocytes; B lymphocytes; ionizing radiation; DNA damage

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2008
• Tom: Vol. 53, No. 4
• Strony: 145--149
• Opis fizyczny: Bibliogr. 17 poz., rys.

Twórcy

autor

Wojewódzka M.

autor

Machaj E. K.

autor

Goździk A.

autor

Iwaneńko T.

autor

Ołdak T.

autor

Kruszewski M.

autor

Pojda Z.

• Department of Radiobiology and Health Protection, Chemistry and Technology, 16 Dorodna Str., 03-195 Warsaw, Poland, Tel.: [phone], Fax: [phone],

Bibliografia

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