Reduction of transgenerational radiation induced genetic damages observed as numerical chromosomal abnormalities in preimplantation embryos by vitamin E

• Autorzy: Salimi M. , Mozdarani H.
• Języki publikacji: EN

Abstrakty

EN

To study the effects of parental gamma-irradiation (4 Gy) of NMRI (Naval Medical Research Institute) mice on the numerical chromosome abnormalities in subsequent preimplantation embryos in the presence of vitamin E (200 IU/kg), super-ovulated irradiated females were mated with irradiated males at weekly intervals in successive 6 weekly periods. About 68 h post coitus, 8-cell embryos were fixed on slides using standard methods in order to screen for abnormalities in chromosome number. In embryos generated by irradiated mice, the frequency of aneuploids dramatically increased compared to control unirradiated groups (p < 0.001), while no significant difference were observed within irradiated groups mated at weekly interval. Administration of vitamin E significantly decreased chromosomal aberrations in all groups (p < 0.05). Data indicate that gamma-irradiation affects spermatogenesis and oogenesis and causes DNA alterations that may lead to chromosome abnormalities in subsequent embryos. Vitamin E effectively reduced the frequency of abnormalities. The way vitamin E reduces genotoxic effects of radiation might be via radical scavenging or antioxidative mechanism.

Słowa kluczowe

EN

paternal irradiation; gamma rays; preimplantation embryo; chromosomal abnormalities; vitamin E; radioprotection

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2008
• Tom: Vol. 53, No. 4
• Strony: 137--143
• Opis fizyczny: Bibliogr. 54 poz., rys.

Twórcy

autor

Salimi M.

autor

Mozdarani H.

• Department of Medical Genetics, Tarbiat Modares University, P. O. Box 14115-111, Tehran, Iran, Tel.: [phone], Fax: [phone],

Bibliografia

• 1. Adler D, Andrae U, Kreise P, Neumann HG, Their R, Wild D (1999) Recommendations for the categorization of germ cell mutagens. Arbeitsmedizin, Sozialmedizin, Umweltmedizin 34:400–403 (in German)
• 2. Arnon J, Meirow D, Lewis-Roness H, Ornoy A (2001) Genetic and teratogenetic effects of cancer treatment of gametes and embryos. Hum Reprod Update 7:394–403
• 3. Athar M, Abdula M, Sultana S, Favier A (1993) Free radicals and trace elements. J Trace Elem Exp Med 6:65–73
• 4. Banetskaia NV, Pavlenko VS, Amvrosev AP (2003) Development of rat embryos after prolonged irradiationof oocytes at the stage of immature follicles. Radiat Biol Radioecol 43:613–617
• 5. Bang D, Lee J, Oh H et al. (2002) Dose incidence relationships on the prenatal effects of gamma-radiation in mice. J Vet Sci 3:7–11
• 6. Barcellos-Hoff MH, Brooks AL (2001) Extracellular signaling through the microenvironment: a hypothesis relating carcinogenesis, bystander effects and genomic instability. Radiat Res 156:618–627
• 7. Bateman AJ (1958) Mutagenic sensitivity of maturing germ cells in the male mouse. Heredity 12:213–232
• 8. Beilsk BH (1982) Chemistry of ascorbic acid chemistry, metabolism and uses. Adv Chem Ser 200:81–100
• 9. Brown MA (1993) Resistance of human erythrocytes containing elevated levels of Vitamin E to radiation-induced hemolysis. Radiat Res 95:303–316
• 10. Burton G, Foster DO (1995) Biological antioxidant. Philos Trans R Soc London, Ser B 331:565–578
• 11. Dasenbrock C, Tillmann T, Ernst H (2005) Maternal effects and cancer risk in the progeny of mice exposed to Xrays before conception. Exp Toxicol Pathol 56:351–360
• 12. Delaney JP, Bonsack M (1992) Intestinal radioprotection by two new agents applied topically. Ann Surg 216:417–421
• 13. Dubrova YE (2003) Radiation induced transgenerational instability. Oncogene 22:7087–7093
• 14. Dyban AP (1991) Reliable techniques for chromosomal preparations from mammalian oocytes and preimplantation embryos. In: Verlinsky Yu, Kuliev A (eds) Preimplantation genetics. Plenum Press, New York, pp 293–298
• 15. Erin AN, Spirin MM, Tabidze LV (1984) Formation of alpha tocopherol complexes with fatty acids. A hypothetical mechanism of stabilization of biomembranes by vitamin E. Biochem Biophys Acta 774:96–102
• 16. Fahrenholtz Sr, Doledn FH (1974) On the quenching of singlet oxygen by alpha tocopherol. Photochem Photobiol 20:505–509
• 17. Fantappie O, Lodovici M, Fabrizio P et al. (2004) Vitamin E protects DNA from oxidative damage in human hepatocellular carcinoma cell lines. Free Radical Res 38:751–759
• 18. Felemovicius I, Bonsack ME, Baptista ML, Deleney JP (1995) Intestinal radioprotection by vitamin E (alphatocopherol). Ann Surg 222:504–510
• 19. Forand A, Dutrillaux B, Bernardino-Sgheri J (2004) Gamma-H2AX expression pattern in non-irradiated mouse germ cells and after low dose gamma-radiation: relationships between chromatid breaks and DNA double strand break. Biol Reproduct 71:643–649
• 20. Hugenholtz AP, Bruce WR (1983) Radiation induction of mutations affecting sperm morphology in mice. Mutat Res 107:177–185
• 21. Jacquet P (2004) Sensitivity of germ cells and embryos to ionizing radiation. J Biol Regul Homeost Agents 18:106–114
• 22. Jacquet P, Adriaens I, Buset J, Neefs M, Vankerkom J (2005) Cytogenetic studies in mouse oocytes irradiated in vitro at different stages of maturation, by use of an early preantral follicle culture system. Mutat Res 583:168–177
• 23. Jacquet P, de Saint-George L, Buset J, Baatout S, Vankerkom J, Baugnet-Mahieu L (1997) Cytogenetic effects of X-rays in the guinea pig female germ cells. II.The maturing oocyte. Mutat Res 391:193–199
• 24. Kamiguchi Y, Tateno H (2002) Radiation- and chemicalinduced structural chromosome aberrations in human spermatozoa. Mutat Res 504:183–191
• 25. Karslioglu I, Ertekin MV, Kocer I et al. (2004) Protective role of intramusculary administered vitamin E on the levels of lipid peroxidation and the activities of antioxidant enzymes in the lens of rats made cataractous with gamma-irradiation. Eur J Ophthalmol 14:478–485
• 26. Konopacka M, Rzeszowska-Wolny J (1998) Modifying effect of vitamins C, E and beta-carotene against gamma-ray induced DNA damage in mouse cells. Mutat Res 417:85–94
• 27. Kumar KS, Ghosh SP, Berbee M, Fu Q, Kao TC, Jensen M. H (2007) Tocols as possible radioprotectants for low level radiation exposure. In: Abstract book of the 6th Lowrad Conf, 17–20 October 2007, Budapest, Hungary, p 75
• 28. Kumar KS, Srinivasan V, Toles R, Jobe L, Seed TM (2002) Nutritional approaches to radioprotection: vitamin E. Military Medicine 167:57–59
• 29. Kumar KS, Srinivasan V, Toles RE, Miner VL, Seed TM (2003) Recovery of reticulocytes and prevention of radiation-induced weight loss in mice by gamma-tocotrienol: possible application to cancer therapy. In: Book of abstracts of the 12th Int Congress of Radiation Research, 17–22 August 2003, Brisbane, Australia, p 153
• 30. Kumar KS, Vaishnav YN, Weiss JF (1988) Radioprotection by antioxidant enzymes and enzyme mimetics. Pharmacol Therapeut 39:301–309
• 31. Laurent C, Voisin P, Pouget JP (2006) DNA damage in cultured skin microvascular endothelial cells exposed to gamma rays and treated by the combination pentoxifyline and alpha-tocopherol. Int J Radiat Biol 82:309–321
• 32. Massay JB, Rovan SS, Povnal HJ (1982) Interaction of vitamin E with satured phospholipid bilayer. Biochem Biophys Res Commun 106:842–847
• 33. Mastuda Y, Tobari I, Yamada T (1985) Studies on chromosome aberrations induced in the eggs of mice fertilized in vitro after irradiation. I. Chromosome aberrations induced in sperm after X-irradiation. Mutat Res 178:113–117
• 34. Mastuda Y, Tobari I (1989) Repair capacity of fertilized mouse eggs for X-ray damage induced in sperm and mature oocytes. Mutat Res 210:35–47
• 35. Mehandjiev A, Vassilev G, Yonova P (1993) Radioprotective effect of thiourea derivatives. Biotechnol Biotechnolog Equip 7:66–70
• 36. Meistrich ML, Hunter NR, Suzuki N,Trostle PK, Withers HR (1978) Gradual regeneration of mouse testicular stem cells after exposure to ionizing radiation. Radiat Res 74:349–362
• 37. Mozdarani H, Salimi M (2006) Numerical chromosome abnormalities in 8-cell embryos generated from gammairradiated male mice in the absence and presence of vitamin E. Int J Radiat Biol 82:817–822
• 38. Nomura T (1986) Further studies on X-ray and chemically induced germ-line alterations causing tumors and malformations in mice. In: Ramel C, Lambert B, Magnusson J. (eds) Genetic toxicology of environmental chemicals. Part B. Genetic effects and applied mutagenesis. Liss,New York, pp 13–20
• 39. Olson SB, Magenis RE (1988) Preferential paternal origin of de novo structural chromosome rearrangments. In: Daniels A (ed) Progress and topics in cytogenetics, the cytogenetics of mammalian autosomal rearrangements. Vol. 8. Liss, New York, pp 585–599
• 40. Palyga GF (2002) Embryogenesis and early postnatal ontogenesis of posterity of two generations of female Wistar rats, depending on the time of their fertilization after low dose radiation exposure. Radiat Biol Radioecol 42:390–394
• 41. Pellestor F, Girardet A, Andreo B, Amal F, Humeau C (1994) Relationship between morphology and chromosomal constitution in human preimplantation embryos. Mol Reprod Dev 39:141–146
• 42. Rastgoo J (1997) Reduction of tratogenic effects of gamma radiation by using vitamin E as a radioprotector. MSc thesis. School of Medical Science, Tarbiat Modares University, Tehran, Iran
• 43. Reichert W, Buselmaier W, Vogel F (1984) Elimination of X-ray induced chromosomal aberration in the progeny of female mice. Mutat Res 139:87–94
• 44. Santalo J, Veiga A, Calafel JM et al. (1995) Evaluation of cytogenetic analysis for clinical preimplantation diagnosis. Fertility and Sterility 64:44–50
• 45. Sarma L, Kesavan PC (1993) Protective effects of vitamins C and E against gamma-ray-induced chromosomal damage. Int J Radiat Biol 63:759–764
• 46. Searl AG, Beechey CV (1974) Sperm-count, egg fertilization and dominant lethality after X-irradiation of mice. Mutat Res 22:63–72
• 47. Seifter E, Rettura G, Padawer J et al. (1984) Morbidity and mortality reduction by supplemental vitamin A or beta-carotene in CBA mice given total-body gammaradiation. J Natl Can Inst 73:1167–1177
• 48. Sohier M, El-Nahas Mathar FE, Mohammad AA (1993) Radioprotective effect of vitamins C and E. Radiat Res 301:143–147
• 49. Songthaveesin C, Saikhun J, Kitiyanant Y, Pavasuthipaisit K (2004) Radio-protective effect of vitamin E on spermatogenesis in mice exposed to gamma-irradiation: a flow cytometric study. Asian J Androl 6:331–336
• 50. Srinivasan V, Weiss JF (1992) Radioprotection by vitamin E: injectable vitamin E administered alone or with WR-3689 enhances survival of irradiated mice. Int J Radiat Oncol Biol Phys 23:841–845
• 51. Sugiyama M, Tsuzuki K, Mastumoto K, Ogura R (1992) Effects of vitamin E on cytotoxicity, DNA single strand breaks, chromosomal aberrations, and mutation in Chinese hamster V-79 cells exposed to ultra violet-b light. Photochem Photobiol 56:31–34
• 52. Tusell L, Alvarez R, Caballin MR et al. (1995) Induction of micronuclei in human sperm-hamster egg hybrids at the two cell stage after in vitro gamma-irradiation of human spermatozoa. Environ Mol Mutagen 26:315–323
• 53. Weiss JF, Kumar KS, Walden TL, Neta R, Landauer MR, Clark EP (1990) Advances in radioprotection through the use of combined agent regimens. Int J Radiat Biol 57:709–722
• 54. Weiss JF, Landauer ML (2000) Radioprotection by antioxidants. Ann NY Acad Sci 899:44–60