Low Molecular Mass Inhibitors of Bovine beta-Trypsin Containing Binding Loop Fragment of CMTI-III. Synthesis and Evaluation of Inhibitory Activity

• Autorzy: Frąszczak P. , Lis K. , Jaśkiewicz A. , Miecznikowska H. , Rolka K. , Kupryszewski G.
• Języki publikacji: EN

Abstrakty

EN

Three peptides with sequences related to the binding loop of Cucurbita maxima trypsin inhibitor III were obtained: [G3,GIO]CMTI-HI (1-10) (1), [A1,G3,A9,G10]MTlII (1-10) (2) and c(K-A-A-P-R-I-L-M-K-Y-A-E) (3). All peptides were synthesized by the solid-phase methodusing the Fmoc/Bu t procedure. Peptide 1 revealed a relatively high inhibitory activity (association equilibrium constant with bovine beta-trypsin Ka = 4 x 10 9 [M -1]). Significantly lower activity (Ka = 3.6x l O4 [M -1) was obtained for peptide 3. Peptide 2 appeared to be inactive.

Słowa kluczowe

EN

peptidic trypsin inhibitors; solid-phase peptide synthesis

• Wydawca: Polskie Towarzystwo Chemiczne
• Czasopismo: Polish Journal of Chemistry
• Rocznik: 2001
• Tom: Vol. 75, nr 12
• Strony: 1863--1868
• Opis fizyczny: Bibliogr. 16 poz., rys.

Twórcy

autor

Frąszczak P.

autor

Lis K.

autor

Jaśkiewicz A.

autor

Miecznikowska H.

autor

Rolka K.

autor

Kupryszewski G.

• Faculty of Chemistry, University of Gdańsk, Sobieskiego 18, 80-952 Gdańsk, Poland