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Evaluation of Boar Spermatozoa Motility by Pulsed Field Gradient NMR

Identyfikatory
Warianty tytułu
Konferencja
1st Symp. on NMR in Chemistry, Biology and Medicine; 8-10 Semptember, Warsaw, Poland. Issue dedicated to honour Prof. Witanowski
Języki publikacji
EN
Abstrakty
EN
The evaluation of spermatozoa motility, viability andmorphology is an essential parameter in the examination of sperm and in the establishment of correlations between sperm quality and fertility. Until now, assessment of sperm quality has been based on subjective evaluation of parameters, such as motility and viability, and on objective parameters, such as semen concentration and morphology abnormalities. When subjective optical microscopic evaluation was used in humans and animals, variations of 30 to 60% have been reported in the estimation of the motility parameters of the same ejaculates. Pulsed field gradient nuclear magnetic resonance (PFG-NMR) techniques have been presented here to demonstrate the potential to study flow and transport processes in complex systems. In this paper we present the results of PFG-NMR obtained for a number of samples of boar spermatozoa and discuss whether this method can be useful for fast and reliable spermatozoa motility evaluation.
Słowa kluczowe
Rocznik
Strony
1075--1082
Opis fizyczny
Bibliogr. 14 poz., rys.
Twórcy
autor
autor
autor
autor
  • 1H. Niewodniczański Institute of Nuclear Physics PAS, Radzikowskiego 152, 31-342 Kraków, Poland
Bibliografia
  • 1. Neuwinger J., Behre H.M. and Nieschlag E., Fertil Steril, 54, 308 (1990).
  • 2. Parkinson T.J., Veterinary J., 168, 215 (2004).
  • 3. Iguer-Ouada M. and Verstegen J.P., Theriogenology, 55, 733 (2001).
  • 4. Verstegen J., Iguer-Ouada M. and Onclin K., Theriogenology, 57, 149 (2001).
  • 5. Amann R.P., J. Androl, 10, 89 (1989).
  • 6. Donnelly E.T., Lewis S.E., McNally J.A. and Thompson W., Fertil Steril, 70, 305 (1998).
  • 7. Budworth P.R., Amann R.P. and Hammerstedt R.H., J. Dairy Sci., 70, 1927 (1987).
  • 8. Burkman L.J., Fertil Steni, 55, 363 (1991).
  • 9. Saacke R.G.,DeJarnetteJ.M.,Nebel R.L.and NadirS.,Prac. Soc. Theriogenol.,San Diego, 56 (1991)
  • 10. Saacke R.G., Nadir S. and Nebel R.L., Theriogenology, 41, 45 (1994).
  • 11. Reed H.C.B., Johnson L.A. and Larsson K., Proc. 1st Int.. Conf. Deep Freezing of Boar Semen, Swedish Univ. Agric. Sciences, Uppsala, pp. 225 (1985).
  • 12. Callaghan P.T., Magn. Reson. Imaging, 14, 701 (1996).
  • 13. Węglarz W.P., Adamek D., Markiewicz J., Skórka T., Kulinowski P. and Jasinski A., Solid State Nucl. Magn. Reson., 25, 88 (2004).
  • 14. Paoli H., Methivier A., Jobic H., Krause C., Pfeifer H., Stallmach F. and Kaerger J., Microporous and Mesoporous Materials, 55, 147 (2002).
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BUJ6-0007-0008
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