Efficiency of a 252Cf source in normal or in B-10 enriched lymphocytes evaluated by SCGE assay, classical cytogenetics and FISH technique

• Autorzy: Cebulska-Wasilewska A. , Niedźwiedź W. , Florjan D. , Schneider K. , Kopec M. , Kreft A.
• Języki publikacji: EN

Abstrakty

EN

Biological effectiveness of a californium-252 source was evaluated after irradiations in vitro of normal or pretreated cells with compound enriched in the B-10 ion (Na210B12H11SH also known as BSH) in order to check the possibility of any enhancement effect due to the process of boron neutron capture. Peripheral blood lymphocytes were used as a model for human cells. Human blood samples or isolated lymphocytes were irradiated with the isotopic source of 252Cf, at the Faculty of Physics and Nuclear Techniques at the University of Mining and Metallurgy, Kraków, (both the neutron source and the samples were placed in an "infinite" polyethylene block). DNA and chromosomal damage were studied to compare the biological effectiveness of irradiation. Single cell gel electrophoresis also known as the Comet assay was done to investigate the DNA damage. Classical cytogenetic analysis was applied to assess the frequencies of unstable aberrations (dicentrics, rings and acentric fragments). To evaluate the frequencies of stable aberrations the fluorescence in situ hybridisation (FISH) with probes for chromosomes 1, 4 (14,3% of the whole genome) was performed. Linear (or close to linear) increases with radiation doses were observed for the DNA damage and aberration frequencies in lymphocytes both untreated or pretreated with BSH. Levels of translocations evaluated for the whole genome were comparable with the frequencies of dicentrics and rings. No significant differences were detected due to radiation dose in the frequencies of sister chromatid exchanges (SCE) detected in the second mitosis. Statistically no significant differences were observed in various biological end-points between normal or boron pre-treated cells.

Słowa kluczowe

EN

BNC reaction; BSH o 252Cf; DNA and chromosomal damage; FISH; lymphocytes

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2001
• Tom: Vol. 46, nr 2
• Strony: 41--49
• Opis fizyczny: Bibliogr. 21 poz., rys.

Twórcy

autor

Cebulska-Wasilewska A.

• University of Mining and Metallurgy, Faculty of Nuclear Physics and Techniques, A. Mickiewicza 30 Str., 30-059 Kraków, Poland

autor

Niedźwiedź W.

• Henryk Niewodniczański Institute of Nuclear Physics, Department of Radiation and Environmental Biology, Radzikowskiego 152 Str., 31-342 Krakow, Poland, Tel.: [phone] ext. 322, Fax: [phone]

autor

Florjan D.

• Henryk Niewodniczański Institute of Nuclear Physics, Department of Radiation and Environmental Biology, Radzikowskiego 152 Str., 31-342 Krakow, Poland, Tel.: [phone] ext. 322, Fax: [phone]

autor

Schneider K.

• Henryk Niewodniczański Institute of Nuclear Physics, Department of Radiation and Environmental Biology, Radzikowskiego 152 Str., 31-342 Krakow, Poland, Tel.: [phone] ext. 322, Fax: [phone]

autor

Kopec M.

• Henryk Niewodniczański Institute of Nuclear Physics, Department of Radiation and Environmental Biology, Radzikowskiego 152 Str., 31-342 Krakow, Poland, Tel.: [phone] ext. 322, Fax: [phone]

autor

Kreft A.

• University of Mining and Metallurgy, Faculty of Nuclear Physics and Techniques, A. Mickiewicza 30 Str., 30-059 Kraków, Poland

Bibliografia

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