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Biological effectiveness of a californium-252 source was evaluated after irradiations in vitro of normal or pretreated cells with compound enriched in the B-10 ion (Na210B12H11SH also known as BSH) in order to check the possibility of any enhancement effect due to the process of boron neutron capture. Peripheral blood lymphocytes were used as a model for human cells. Human blood samples or isolated lymphocytes were irradiated with the isotopic source of 252Cf, at the Faculty of Physics and Nuclear Techniques at the University of Mining and Metallurgy, Kraków, (both the neutron source and the samples were placed in an "infinite" polyethylene block). DNA and chromosomal damage were studied to compare the biological effectiveness of irradiation. Single cell gel electrophoresis also known as the Comet assay was done to investigate the DNA damage. Classical cytogenetic analysis was applied to assess the frequencies of unstable aberrations (dicentrics, rings and acentric fragments). To evaluate the frequencies of stable aberrations the fluorescence in situ hybridisation (FISH) with probes for chromosomes 1, 4 (14,3% of the whole genome) was performed. Linear (or close to linear) increases with radiation doses were observed for the DNA damage and aberration frequencies in lymphocytes both untreated or pretreated with BSH. Levels of translocations evaluated for the whole genome were comparable with the frequencies of dicentrics and rings. No significant differences were detected due to radiation dose in the frequencies of sister chromatid exchanges (SCE) detected in the second mitosis. Statistically no significant differences were observed in various biological end-points between normal or boron pre-treated cells.
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Tom
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41--49
Opis fizyczny
Bibliogr. 21 poz., rys.
Twórcy
autor
- University of Mining and Metallurgy, Faculty of Nuclear Physics and Techniques, A. Mickiewicza 30 Str., 30-059 Kraków, Poland
autor
- Henryk Niewodniczański Institute of Nuclear Physics, Department of Radiation and Environmental Biology, Radzikowskiego 152 Str., 31-342 Krakow, Poland, Tel.: +4812/ 6370222 ext. 322, Fax: +4812/ 6375441
autor
- Henryk Niewodniczański Institute of Nuclear Physics, Department of Radiation and Environmental Biology, Radzikowskiego 152 Str., 31-342 Krakow, Poland, Tel.: +4812/ 6370222 ext. 322, Fax: +4812/ 6375441
autor
- Henryk Niewodniczański Institute of Nuclear Physics, Department of Radiation and Environmental Biology, Radzikowskiego 152 Str., 31-342 Krakow, Poland, Tel.: +4812/ 6370222 ext. 322, Fax: +4812/ 6375441
autor
- Henryk Niewodniczański Institute of Nuclear Physics, Department of Radiation and Environmental Biology, Radzikowskiego 152 Str., 31-342 Krakow, Poland, Tel.: +4812/ 6370222 ext. 322, Fax: +4812/ 6375441
autor
- University of Mining and Metallurgy, Faculty of Nuclear Physics and Techniques, A. Mickiewicza 30 Str., 30-059 Kraków, Poland
Bibliografia
- 1. Anderson D, Hughes AA, Cebulska-Wasilewska A, Wierzewska A, Kasper E (1996) Biological monitoring of workers exposed to emissions from petroleum plants. J Environ Health Persp 194;3:609–613
- 2. Anderson D, Yu T-W, Philips BJ, Schmezer P (1994) The effects of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the “comet assay”. Mutat Res 307:261–271
- 3. Barth RF, Soloway AH, Brugger RM (1996) Boron neutron capture therapy of brain tumors: past history, current status and future potential. Cancer Investigation 14;6:534–550
- 4. Bewley DK (1989) The physics and radiobiology of fast neutron beams. Medical Science Series. IOP Publishing Ltd, Adam Hilger, Bristol and New York
- 5. Biological dosimetry, chromosome abberration analysis for dose assessment (1986) IAEA Technical Reports Series, No. 260. IAEA, Vienna 48 A. Cebulska-Wasilewska et al.
- 6. Capała J, Makar MS, Coderre JA (1996) Accumulation of boron in malignant and normal calls incubated in vitro boronphenylalanine, marcaptoborane and boric acid. Radiat Res 146:554–560
- 7. Cebulska-Wasilewska A, Niedźwiedź W, Nowak D, Pawliński R (1996) Hydrogen peroxide and radiation induced DNA damage investigated by the “comet assay”. Nukleonika 41;2:33–42
- 8. Cebulska-Wasilewska A, Niedźwiedź W, Wierzewska A, Capała J, Coderre JA (2000) Biological efficiency of the therapeutic neutron beam in the DNA and cytogenetic damage induction in presence and absence of 10B. Report IFJ No. 1869/B
- 9. Cebulska-Wasilewska A, Nowak D, Niedźwiedź W (1998) Correlations between DNA and cytogenetic damage induced after chemical treatment and radiation. Mutat Res 421:83–91
- 10. Cebulska-Wasilewska A, Schneider K, Kim JK (2001) Studies on californium neutrons efficiency in the induction of gene mutations in normal or pretreated with boron ion Trad-SH cells. Mutat Res 474:57–70
- 11. Cebulska-Wasilewska A, Wierzewska A, Kasper E, Huiskamp R, Lloyd D (1997) RBE of 5.6 MeV and fission neutrons assessed from chromosomal aberrations in human blood lymphocytes. Polish J Med Phys & Eng 3;1(7):1–9
- 12. Florjan D, Niedżwiedź W, Schneider K, Kreft A, CebulskaWasilewska A (1999) Application of SCGE assay, classical cytogenetics and FISH for studying in vitro californium-252 neutrons irradiations and BSH pretreatment on human lymphocytes. Report IFJ No.1831/B
- 13. Gabel D, Moss R (eds) (1992) Boron neutron capture therapy: toward clinical trials of glioma treatment. Plenum Press, New York and London
- 14. Lamberti P, Bigatti Ponzetto P, Ardito G (1983) Cell kinetics and sister chromatid-exchange frequency in human lymphocytes. Mutat Res 130:193–199
- 15. Lindholm C, Luomahaara S, Koivistoinen A, Ilus T, Edwards AA, Salomaa S (1998) Comparison of dose-response curves for chromosomal aberrations established by chromosome painting and conventional analysis. Int J Radiat Biol 74;1:27–34
- 16. Lindholm C, Salomaa S, Tekkel M, Paile W, Koivistoinen A, Ilus T, Veidebaum T (1996) Biodosimetry after accidental radiation exposure by conventional chromosome analysis and FISH. Int J Radiat Biol 70;6:647–656
- 17. Lucas JN, Awa A, Straume T et al. (1992) Rapid translocation frequency analysis in humans decades after exposure to ionising radiation. Int J Radiat Biol 62:53–63
- 18. Lucas JN, Chen AM, Sachs RK (1996) Theoretical predictions on the equality of radiation-produced dicentrics and translocation detected by chromosome painting. Int J Radiat Biol 69;2:145–153
- 19. Pöller F, Bauch T, Sauerwein W, Böcker W, Wittig A, Streffer C (1996) Comet assay study of DNA damage and repair of tumour cells following boron neutron capture irradiation with fast d(14)+Be neutrons. Int J Radiat Biol 70;5:593–602
- 20. Prasad KN (ed) (1995) Handbook of radiobiology. CRC Press Inc., Boca Raton, Florida
- 21. Tanaka K, Hoshi M, Sawada S, Kamada N (1994) Effects of 252Cf neutrons, transmitted through an iron block on human lymphocyte chromosome. Int J Radiat Biol 66;4:391–397
Typ dokumentu
Bibliografia
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bwmeta1.element.baztech-article-BUJ6-0006-0085