Influence of the photosynthetic pathway on the hydrogen isotopic profile of glucose

• Autorzy: Zhang B. , Billault I. , Li X. , Mabon F. , Remaud G. , Martin M.
• Konferencja: Proceedings of the International Conference “2001 an Isotope Odyssey: New Application for a New Millenium", June 24-29, 2001, Zakopane, Poland
• Języki publikacji: EN

Abstrakty

EN

The SNIF-NMR method (site-specific natural isotope fractionation studied by Nuclear Magnetic Resonance) was used to examine the isotopic profile of glucoses derived from plants with different photosynthetic pathways. It is shown that the type of photosynthetic metabolism, either C3 (beet-root, orange, grape), C4 (maize, sugar-cane) or CAM (pineapple), exerts a strong influence on the deuterium distribution in the sugar molecules. The isotopic profile also depends, secondarily, on the physiological status of the precursor plant. Consequently, the isotopic fingerprint of glucose may be a rich source of information in mechanistic comparisons of metabolic pathways. Moreover, from an analytical point of view, it may provide complementary criteria with respect to the ethanol probe for origin inference of sugars.

Słowa kluczowe

EN

deuterium; glucose; isotope ratio; plant metabolism; SNIF-NMR

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2002
• Tom: Vol. 47,suppl.1
• Strony: 63--65
• Opis fizyczny: Bibliogr. 8 poz., rys.

Twórcy

autor

Zhang B.

• Laboratoire d'Analyse Isotopique et Electrochimique de Métabolismes, CNRS UMR 6006 Université de Nantes, 2 rue de la Houssiniére, 44322 Nantes, France, Tel.: [phone], Fax: [phone]

autor

Billault I.

• Laboratoire d'Analyse Isotopique et Electrochimique de Métabolismes, CNRS UMR 6006 Université de Nantes, 2 rue de la Houssiniére, 44322 Nantes, France, Tel.: [phone], Fax: [phone]

autor

Li X.

• Laboratoire d'Analyse Isotopique et Electrochimique de Métabolismes, CNRS UMR 6006 Université de Nantes, 2 rue de la Houssiniére, 44322 Nantes, France, Tel.: [phone], Fax: [phone]

autor

Mabon F.

• Laboratoire d'Analyse Isotopique et Electrochimique de Métabolismes, CNRS UMR 6006 Université de Nantes, 2 rue de la Houssiniére, 44322 Nantes, France, Tel.: [phone], Fax: [phone]

autor

Remaud G.

• Eurofins Scientific, rue P. A. Bobierre, BP 72304, 44323 Nantes Cedex 3, France

autor

Martin M.

• Eurofins Scientific, rue P. A. Bobierre, BP 72304, 44323 Nantes Cedex 3, France

Bibliografia

• 1. Allison GB, Gat JR, Leaney FWJ (1985) The relationship between deuterium and oxygen-18 delta values in leaf water. Chem Geol 58:145–156
• 2. Martin GJ, Martin ML (1981) Deuterium labeling at the natural abundance level as studied by high field quantitative 2H-NMR. Tetrahedron Lett 22:3525–3528
• 3. Martin GJ, Martin ML (1990) Deuterium NMR in the study of Site specific Natural Isotope Fractionation (SNIF-NMR). In: Diehl P, Fluck E, Günther H, Kosfeld R, Seelig J (eds) NMR basic principles and progress, vol. 23. Springer-Verlag, Berlin, pp 1–61
• 4. Rossmann A, Butzenlechner M, Schmidt HL (1991) Evidence for a nonstatistical carbon isotope distribution in natural glucose. Plant Physiol 96:609–614
• 5. Schleucher J, Vanderveer P, Markley JL, Sharkey TD (1999) Intramolecular deuterium distributions reveal disequilibrium of chloroplast phosphoglucose isomerase. Plant Cell Environ 22:525–533
• 6. Smith BN, Ziegler H (1990) Isotopic fractionation of hydrogen in plants. Bot Acta 103:335–342
• 7. Zhang BL, Quemerais B, Martin ML, Martin GJ, Williams JM (1994) Determination of the natural deuterium distribution in glucose from plants having different photosynthetic pathways. Phytochem Analysis 5:105–110
• 8. Zhang BL, Billault I, Li XL, Mabon F, Remaud G, Martin ML (2002) Hydrogen isotopic profile in the characterization of sugars. Influence of the metabolic pathway. J Agric Food Chem (in press)