Low frequency of spontaneous rearrangements during plasmid incorporation in CHO-K1 mutant cells defective in DNA repair

• Autorzy: Kruszewski M. , Kruszewska H. , Mori M. , Eguchi-Kasai K. , Inaba H. , Zakierska I. , Hayata I.
• Języki publikacji: EN

Abstrakty

EN

pLrec plasmid DNA was introduced into Chinese hamster cell lines defective in DNA repair (Pa13, Pb4, xrs6) and the parental CHO-K1 cell line. Clones with stable integrated plasmid were isolated and integrity of the incorporated DNA was checked by Southern blotting and PCR. Intact pLrec plasmid was found in 11% of the isolated CHO-K1 clones. In contrast, intact plasmid copies were found in 68.8%, 50%, 35.7% clones of Pa13, Pb4 and xrs6 cell lines, respectively. We conclude that certain DNA repair defects may facilitate intact plasmid integration. The higher frequency of integration of the intact vector into the genome of cell lines defective in DNA repair as compared to the parental cell line points to two possibilities, not mutually exclusive: (1) these cells possess a mechanism that facilitates the process of plasmid incorporation and hence, plasmid DNA is incorporated into the genome before extrachromosomal recombination takes place; (2) the vector is inserted into a less recombination prone site in the genome.

Słowa kluczowe

EN

CHO-K1; Pa13; Pb4; plasmid incorporation; xrs6

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2002
• Tom: Vol. 47, nr 1
• Strony: 7--11
• Opis fizyczny: Bibliogr. 26 poz., rys.

Twórcy

autor

Kruszewski M.

• Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, 16 Dorodna Str., 03-195 Warsaw, Poland, Tel.: [phone], Fax: [phone]
• M.Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, 5 W. K. Roentgena Str., 02-781 Warsaw, Poland

autor

Kruszewska H.

• Drug Institute, 33/35 Che∏mska Str., 00-725 Warsaw, Poland

autor

Mori M.

• National Institute of Radiological Sciences, 9-1, 4-Chome, Anagawa, Inage-ku, Chiba 263, Japan

autor

Eguchi-Kasai K.

• National Institute of Radiological Sciences, 9-1, 4-Chome, Anagawa, Inage-ku, Chiba 263, Japan

autor

Inaba H.

• National Institute of Radiological Sciences, 9-1, 4-Chome, Anagawa, Inage-ku, Chiba 263, Japan

autor

Zakierska I.

• Polfa-¸ Łódź S.A., 43/55 Drewnowska Str., 91-002 ¸Łódź Poland

autor

Hayata I.

• National Institute of Radiological Sciences, 9-1, 4-Chome, Anagawa, Inage-ku, Chiba 263, Japan

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