Pro-angiogenic effects of X-rays on murine endothelial cells

• Autorzy: Lisiak E. , Dziekiewicz M. , Meineke V. , Bilski M. , Janiak M.
• Konferencja: Proceedings of the XIII Scientific Meeting of the Polish Radiation Research Society, Memorial to Maria Skłodowska-Curie, 13-16 September 2004, Łódź, Poland
• Języki publikacji: EN

Abstrakty

EN

Recently, significant attention has been paid to the possibility of thwarting cancer progression by inhibition of neoangiogenesis (formation of new blood vessels) in growing tumors. Although general mechanisms of angiogenesis have been elucidated, virtually nothing is known about the effects of low doses of ionizing radiation on pro-angiogenic properties of endothelial cells. In the present study, we evaluated the effects of a low (0.2 Gy), intermediate (1 Gy), and high (4 Gy) doses of X-rays on a few angiogenesis-related parameters of isolated murine endothelial cells. We show here that 24 to 48 hours after irradiation with 0.2 Gy the cell proliferation was inhibited to a similar extent as after the exposure to 1 Gy. Also, adhesion of the 0.2 Gy-irradiated cells to both gelatin and MatrigelŽ was inhibited 24 hours post-exposure, whereas irradiation with 1 or 4 Gy resulted in the increased adhesion of the cells to these substrata. Similar effects were observed during the "wound" migration assay. Finally, 24 hours after exposure of the cells to 0.2 Gy of X-rays, the surface expression of the â3 integrin subunit was down-regulated, whereas irradiations with 1 and 4 Gy of X-rays resulted in the significantly elevated expression of this subunit. These results indicate that proliferating endothelial cells are sensitive in vitro to relatively low doses of ionizing radiation

Słowa kluczowe

EN

angiogenesis; ionizing radiation; cell adhesion; integrins

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2005
• Tom: Vol. 50,suppl.2
• Strony: 17--20
• Opis fizyczny: Bibliogr. 15 poz., rys.

Twórcy

autor

Lisiak E.

• Military Institute of Hygiene and Epidemiology, Department of Radiobiology and Radiation Protection, 4 Kozielska Str., 01-163 Warsaw, Poland, Tel.: [phone], Fax: [phone]

autor

Dziekiewicz M.

• Military Institute of Health Services, Department of General, Oncological and Vascular Surgery, 128 Szaserów Str., 00-909 Warsaw, Poland

autor

Meineke V.

• Bundeswehr Institute of Radiobiology, 11 Neuherbergstrasse, 80937 Munich, Germany

autor

Bilski M.

• Military Institute of Hygiene and Epidemiology, Department of Radiobiology and Radiation Protection, 4 Kozielska Str., 01-163 Warsaw, Poland, Tel.: [phone], Fax: [phone]

autor

Janiak M.

• Military Institute of Hygiene and Epidemiology, Department of Radiobiology and Radiation Protection, 4 Kozielska Str., 01-163 Warsaw, Poland, Tel.: [phone], Fax: [phone]

Bibliografia

• 1. Cordes N, Beineke C, Plasswilm L, Van Beuningen D (2004) Irradiation and various cytotoxic drugs enhance tyrosine phosphorylation and beta(1)-integrin clustering in human A549 lung cancer cells in a substratum-dependent manner in vitro. Strahlenther Onkol 180:157−164
• 2. Cordes N, Blease MA, Meineke V, Van Beuningen D (2002) Ionizing radiation induces up-regulation of beta 1-integrin in human lung tumor cell lines in vitro. Int J Radiat Biol 78:347−357
• 3. Cordes N, Hansmeier B, Beineke C, Meineke V, Van Beuningen D (2003) Irradiation differentially affects substratum-dependent survival, adhesion, and invasion of glioblastoma cell lines. Br J Cancer 89:2122−2132
• 4. Dong QG, Bernasconi S, Lostaglio S et al. (1997) A general strategy for isolation of endothelial cells from murine tissues. Arterioscler Thromb Vasc Biol 17:1599−1604
• 5. Folkman J (2002) Role of angiogenesis in tumor growth and metastasis. Semin Oncol 29:15−18
• 6. Hildebrandt G, Maggiorella L, Rodel F, Rodel V, Willis D, Trott KR (2002) Mononuclear cell adhesion and cell adhesion molecule liberation after X-irradiation of activated endothelial cells in vitro. Int J Radiat Biol 78:315−325
• 7. Joussen AM, Kruse FE, Oetzel D, Voelcker HE (2000) Irradiation for inhibition of endothelial cell growth in vitro. Ophthalmic Res 32:222−228
• 8. Meineke V, Gilbertz KP, Schilperoort K et al. (2002) Ionizing radiation modulates cell surface integrin expression and adhesion of COLO-320 cells to collagen and fibronectin in vitro. Strahlenther Onkol 178:709−714
• 9. Raicu M, Vral A, Thierens H, De Ridder L (1993) Radiation damage to endothelial cells in vitro, as judged by the micronucleus assay. Mutagenesis 8:335−339
• 10. Rhee JG, Lee I, Song CW (1986) The clonogenic response of bovine aortic endothelial cells in culture to radiation Radiat Res 106:182−189
• 11. Sakamoto K, Miyamoto M, Watabe N, Takai Y (1987) Fundamental and clinical studies of low-dose total body irradiation in tumor control. Gan No Rinsho 33:1633−1638
• 12. Sonveaux P, Brouet A, Havaux X et al. (2003) Irradiationinduced angiogenesis through the up-regulation of the nitric oxide pathway: implications for tumor radiotherapy. Cancer Res 63:1012−1019
• 13. Szala S, Radzikowski C (1997) Molecular basis of neoplastic angiogenesis. Nowotwory 47:1−19 (in Polish)
• 14. Wachsberger P, Burd R, Dicker AP (2003) Tumor response to ionizing radiation combined with antiangiogenesis or vascular targeting agents: exploring mechanisms of interaction. Clin Cancer Res 9:1957−1966
• 15. Wild-Bode C, Weller M, Rimner A, Dichgans J, Wick W (2001) Sublethal irradiation promotes migration and invasiveness of glioma cells: implications for radiotherapy of human glioblastoma. Cancer Res 61:2744−2750