Stimulatory effects of single low-level irradiations with X-rays on functions of murine peritoneal macrophages

• Autorzy: Cheda A. , Wrembel-Wargocka J. , Nowosielska E. , Janiak M.
• Konferencja: Proceedings of the XIII Scientific Meeting of the Polish Radiation Research Society, Memorial to Maria Skłodowska-Curie, 13-16 September 2004, Łódź, Poland
• Języki publikacji: EN

Abstrakty

EN

A number of epidemiological and experimental data indicate that exposures to low doses of low-LET ionising radiation may trigger the activity of natural anti-tumour immune mechanisms and inhibit tumour growth. In the present study, we assessed the cytotoxic activity and production of nitric oxide, superoxide anions, and tumour necrosis factor-alfa in peritoneal macrophages collected from BALB/c mice exposed to single whole-body irradiations with 0.1, 0.2, or 1.0 Gy X-rays. The results indicate that all the tested parameters were significantly up-regulated in macrophages obtained from mice exposed to 0.1 or 0.2 Gy X-rays but not in those collected from the sham-irradiated and 1.0 Gy-exposed animals.

Słowa kluczowe

EN

low doses; X-rays; macrophages; nitric oxide; cytotoxicity; TNF-alfa

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2005
• Tom: Vol. 50,suppl.2
• Strony: 13--16
• Opis fizyczny: Bibliogr. 19 poz., rys.

Twórcy

autor

Cheda A.

• Military Institute of Hygiene and Epidemiology, Department of Radiobiology and Radiation Protection, 4 Kozielska Str., 01-163 Warsaw, Poland, Tel.: [phone], Fax: [phone]

autor

Wrembel-Wargocka J.

• Military Institute of Hygiene and Epidemiology, Department of Radiobiology and Radiation Protection, 4 Kozielska Str., 01-163 Warsaw, Poland, Tel.: [phone], Fax: [phone]

autor

Nowosielska E.

• Military Institute of Hygiene and Epidemiology, Department of Radiobiology and Radiation Protection, 4 Kozielska Str., 01-163 Warsaw, Poland, Tel.: [phone], Fax: [phone]

autor

Janiak M.

• Military Institute of Hygiene and Epidemiology, Department of Radiobiology and Radiation Protection, 4 Kozielska Str., 01-163 Warsaw, Poland, Tel.: [phone], Fax: [phone]

Bibliografia

• 1. Al-Sarireh B, Eremin O (2000) Tumour-associated macrophages (TAMS): disordered function, immune suppression and progressive tumour growth. J R Coll Surg Edinb 45:1−16
• 2. Cheda A, Wrembel-Wargocka J, Lisiak E, Marciniak M, Nowosielska EM, Janiak MK (2004) Inhibition of the development of pulmonary tumour nodules and stimulation of the activity of NK cells and macrophages in mice by single low doses of low-let radiation. Int J Low Radiat 1:171−179
• 3. Cheda A, Wrembel-Wargocka J, Lisiak E, Nowosielska EM, Marciniak M, Janiak MK (2004) Single low doses of X rays inhibit the development of experimental tumor metastases and trigger the activities of NK cells in mice. Radiat Res 161:335−340
• 4. Cui S, Reichner JS, Mateo RB, Albina JE (1994) Activated murine macrophages induce apoptosis in tumor cells through nitric oxide-dependent or –independent mechanisms. Cancer Res 54:2462−2467
• 5. Farias-Eisner R, Sherman MP, Aeberhard E, Chaudhuri G (1994) Nitric oxide is an important mediator for tumoricidal activity in vivo. Proc Natl Acad Sci USA 91:9407−9411
• 6. Frank J, Born K, Barker JH, Marzi I (2003) In vivo effect of tumor necrosis factor alpha on wound angiogenesis and epithelization. Eur J Trauma 29:208−219
• 7. Hashimoto S, Shirato H, Hosokawa M et al. (1999) The suppression of metastases and the change in host immune response after low-dose total-body irradiation in tumorbearing rats. Radiat Res 151:717−724
• 8. Hosoi Y, Miyachi H, Matsumoto Y et al. (2001) Induction of inter-leukin-1b and interleukin-6 mRNA by low doses of ionizing radiation in macrophages. Int J Cancer 96:270−276
• 9. Hosoi Y, Sakamoto K (1993) Suppressive effect of low dose total body irradiation on lung metastasis: dose dependency and effective period. Radiother Oncol 26:177−179
• 10. Ibuki Y, Goto R (1995) Augmentation of NO production and cytolytic activity of Mφ obtained from mice irradiated with a low dose of γ-rays. J Radiat Res 36:209−220
• 11. Ibuki Y, Goto R (1997) Enhancement of NO production from resident peritoneal macrophages by in vitro gammairradiation and its relationship to reactive oxygen intermediates. Free Radic Biol Med 22:1029−1035
• 12. Ibuki Y, Goto R (1999) Contribution of inflammatory cytokine relase to activation of resident peritional macrophages after in vivo low-dose γ-irradiation. J Radiat Res 40:253−262
• 13. Ibuki Y, Goto R (2004) Ionizing radiation-induced macrophage activation: augmentation of nitric oxide production and its significance. Cell Mol Biol (Noisy-legrand) 50:OL617−OL626
• 14. Ibuki Y, Mizuno S, Goto R (2002) γ-Irradiation-induced DNA damage enhances NO production via NF-kB activation in RAW264.7 cells. Biochim Biophys Acta 1593:159−167
• 15. Iwamoto KS, McBride WH (1994) Production of 13-hydroxyoctadecadienoic acid and tumor necrosis factoralpha by murine peritoneal macrophages in response to irradiation. Radiat Res 139:103−108
• 16. Nathan C (1991) Mechanisms and modulation of macrophage activation. Behring Inst Mitt 88:200−207
• 17. Shinohara H, Yano S, Bucana CD, Fidler IJ (2000) Induction of chemokine secretion and enhancement of contact-dependent macrophage cytotoxicity by engineered expression of granulocyte-macrophage colony-stimulating factor in human colon cancer cells. J Immunol 164:2728−2737
• 18. Wiltrout RH, Herberman RB, Zhang SR et al. (1985) Role of organ-associated NK cells in decreased formation of experimental metastases in lung and liver. J Immunol 134:4267−4275
• 19. Xie K, Fidler IJ (1998) Therapy of cancer metastasis by activation of the inducible nitric oxide synthase. Cancer Metastasis Rev 17:55−75