Identyfikatory
Warianty tytułu
Konferencja
Proceedings of the XIII Scientific Meeting of the Polish Radiation Research Society, Memorial to Maria Skłodowska-Curie, 13-16 September 2004, Łódź, Poland
Języki publikacji
Abstrakty
Human biomonitoring, as a tool to identify health risk from environmental exposures, has gained increasing interest especially in the areas of cancer risk assessment and diseases treatment. Chromosome aberrations resulting from direct DNA breakage or from inhibition of DNA repair or synthesis, measured in peripheral blood lymphocytes, have been used successfully in the assessment of health risk associated to environmental genotoxic exposures. A faster but sensitive and reliable method for detection of DNA damage, or DNA repair capacity, might be crucial to many fields from molecular epidemiology and toxicology to preventive and clinical medicine. There are reports that results of DNA measures with the use of single cell gel electrophoresis (SCGE) correlate, on the one hand, with physical measures of genotoxins, and on the other hand, with cytogenetic damage that is a biomarker associated to the alteration of the health risk. This review is based on studies in which exposure to radiation was applied as a challenging treatment and DNA damage induced and repaired was analyzed with the use of the alkaline version of SCGE assay. Results from studies on susceptibilities and repair competence carried out in various groups of exposed workers, controls, and cancer patients (more than 700 donors) show variability between donors both in a response to challenging treatment and in the efficiency of repair process. Influences of the occupational exposures and factors depending on genotypes or life style on cellular capacities are observed. Discussed results suggest that study in vitro with the challenging cells by radiation exposure and measuring, with the SCGE assay, the DNA damage before and after repair, may develop a good biomarker of the individual susceptibility to various genotoxins and exposures (environmental, occupational, therapeutic). Such a biomarker may have a potential use in a molecular epidemiology and preclinical identification.
Słowa kluczowe
Czasopismo
Rocznik
Tom
Strony
3--8
Opis fizyczny
Bibliogr. 17 poz., rys.
Twórcy
autor
- Department of Radiation and Environmental Biology, H. Niewodniczański Institute of Nuclear Physics Polish Academy of Sciences, 152 Radzikowskiego Str., 31-342 Kraków and Chair of Epidemiology and Preventive Medicine Department, CMUJ, 7 Kopernika St, b7wasile@cyf-kr.edu.pl
Bibliografia
- 1. Anderson D, Yu TW, Philips BJ, Schmezer P (1994) The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the comet assay. Mutat Res 307:261−271
- 2. Anvar WA (1997) Biomarkers of human exposure to pesticides. Environ Health Perspect 105;S4:801−806
- 3. Au WW (2000) Strategies for conducting population monitoring studies. In: Anderson D, Karakaya AE, Sram RJ (eds) Human monitoring after environmental and occupational exposure to chemical and physical agents. Series A: Life Sciences, vol. 313. IOS Press, Amsterdam, pp 86−93
- 4. Au WW, Wilkinson GS, Tyring SK et al. (1996) Monitoring populations for DNA repair deficiency and for cancer susceptibility. Environ Health Perspect 104;S3:579−584
- 5. Bonassi S, Hagmar L, Strömberg U et al. (2000) Chromosomal aberrations in lymphocytes predict human cancer independently of exposure to carcinogens. Cancer Res 60:1619−1625
- 6. Cebulska-Wasilewska A (2003) Response to challenging dose of X-rays as a predictive assay for molecular epidemiology. Mutat Res 544;2-3:289−297
- 7. Cebulska-Wasilewska A, Dyga W, Krasnowolski S, Wierzewska A, Budzanowska E (1999) Monitoring of DNA and cytogenetic damage in lymphocytes from patients with skin diseases. Neoplasma 46:105−106
- 8. Cebulska-Wasilewska A, Marcos R, Piperakis S et al. (2003) Studies on the influence of occupational exposure to pesticides in various countries on the susceptibility and repair capacity of UV induced damage. In: CebulskaWasilewska A, Au WW, Sram RJ (eds) Human monitoring for genetic effects. NATO Science Series, Series I: Life and Behavioural Sciences, vol. 351. IOS Press, Tokyo, pp 186−197
- 9. Cebulska-Wasilewska A, Niedźwiedź W, Nowak D, Anderson D (1998) Correlation between DNA and cytogenetic damage induced by chemical treatment or radiation. Mutat Res 421:83−91
- 10. Cebulska-Wasilewska A, Sram RJ, Binkova B et al. (2004) Influence of the environmental exposure to PAHs on the cellular susceptibility to the induction of DNA damage. Eur J Epidemiology 47:124−125
- 11. Cebulska-Wasilewska A, Wierzewska A, Niżankowska E, Graca B, Hughes JA, Anderson D (1999) Cytogenetic damage and ras p21 oncoprotein levels from patients with chronic obstructive pulmonary disease (COPD), untreated lung cancer and healthy controls. Mutat Res 431:123−131
- 12. Cebulska-Wasilewska A, Wierzewska A, Żabiński Z, Moszczyński P (2001) Studies of occupational exposure to mercury vapours influence on the levels of cytogeneticdamage detected in lymphocytes. Mutat Res 483;S1:5F−5
- 13. Chadwick KH, Leenhouts HP (1981) The molecular theory of radiation biology. Monographs on theoretical and applied genetics, vol. 5. Springer-Verlag, Berlin− Heidelberg−New York
- 14. Dyga W, Drąg Z, Cebulska-Wasilewska A (2002) Influence of occupational exposure to pesticides on the susceptibility and repair capacity of UV and X-rays induced damage. Polish Journal of Environmental Studies 11;S2:56−60
- 15. Farmer PB, Singh R, Kaur B et al. (2003) Molecular epidemiology studies of carcinogenic environmental pollutants. Effects of polycyclic aromatic hydrocarbons (PAHs) in environmental pollution on exogenous and oxidative DNA damage. Mutat Res 544:397−402
- 16. Perera FP (2003) Molecular epidemiology in cancer prevention. In: Jedrychowski WA, Perera FP, Maugeri U (eds) Molecular epidemiology in preventive medicine.Luxembourg, pp 35−55
- 17. Zahm SH, Fraumeni JF, Davis DL (1995) The avoidable causes of cancer. Environ Health Perspect 103;S8:134−139
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BUJ6-0005-0014