Carbon isotope effects in the studies of the mechanism of action of tyrosine phenol-lyase

• Autorzy: Augustyniak W. , Kański R. , Kańska M.
• Konferencja: Proceedings of the IV All-Polish Conference on Radiochemistry and Nuclear Chemistry9-11 May 2005, Kraków-Przegorzały, Poland
• Języki publikacji: EN

Abstrakty

EN

12C/14C kinetic isotope effects on the hydrolytic cleavage of tyrosine to phenol and ammonium pyruvate catalyzed by Citrobacter freundii tyrosine phenol-lyase have been determined in positions 2, 3 and ring-1' of L-tyrosine. The competitive method with dual-label approach was applied with 3',5'-ring 3H as remote label. The results revealed the change of the effect on carbon atom in position 2 during the reaction course from the high normal values to low inverse values. On the other hand, the effect values on 3 and ring-1' position remained constant during the reaction course. The discussion of these results regarding the reaction mechanism is presented.

Słowa kluczowe

EN

12C/14C kinetic isotope effects; tyrosine phenol-lyase

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2006
• Tom: Vol. 51,suppl.2
• Strony: 7--11
• Opis fizyczny: Bibliogr. 11 poz., rys.

Twórcy

autor

Augustyniak W.

autor

Kański R.

autor

Kańska M.

• Department of Chemistry, University of Warsaw, 1 Pasteura Str., 02-093 Warsaw, Poland, Tel.: [phone] ext. 509, Fax: [phone],,

Bibliografia

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