Electron Spin Resonance study of radiation-induced radicals in polycrystalline oligopeptides containing methionine

• Autorzy: Strzelczak G. , Bobrowski K. , Kozubek H. , Michalik J.
• Konferencja: Reactive Intermediates in Sulfur Chemistry Workshop dedicated to Professor Klaus-Dieter Asmus on His 60th birthday August 23-26, 1998, Poznan, Poland
• Języki publikacji: EN

Abstrakty

EN

The radicals formed on g-radiolysis of oligopeptides containing methionine and glycine residues in the polycrystalline state at 77 K and on subsequent warming over the temperature range of 77-293 K were identified using the electron spin resonance (ESR) technique. In all oligopeptides studied, anion peptide radicals and monomeric sulfur radical cations over the temperature range of 77-95 K and decarboxylation and deamination radicals over the temperature range of 180-250 K were detected. In addition, in oligopeptides containing adjacent methionyl residues, three-electron-bonded dimeric sulfur radical cations (S\S)+ were detected at 110 K. In oligopeptides containing N-terminal methionine residue, H-abstraction radicals in the side chain of methionine residue were observed over the temperature range of 180-250 K. H-abstraction radicals at the a-carbon of the C-terminal amino acid residue were detected at 293 K in most of oligopeptides studied, in contrast to Met-Gly-Met and Met-Gly-Met-Met peptides were H-abstraction radicals at the alfa-carbon of the internal glycine residue were obserwed.

Słowa kluczowe

EN

ESR; polycrystalhne oligopeptides; radicals

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2000
• Tom: Vol. 45, nr 1
• Strony: 93--100
• Opis fizyczny: Bibliogr. 39 poz., rys.

Twórcy

autor

Strzelczak G.

autor

Bobrowski K.

autor

Kozubek H.

autor

Michalik J.

• Institute of Nuclear Chemistry and Technology, 16 Dorodna Str., 03-195 Warsaw, Poland