The bystander effect: is reactive oxygen species the driver?

• Autorzy: Szumiel I.
• Języki publikacji: EN

Abstrakty

EN

The paper reviews selected examples of the bystander effect, such as clonogenic survival decrease, chromosomal aberrations and mutations. The similarities and differences between the biological effects in directly targeted and bystander cells are briefly discussed. Also reviewed are the experimental data which support the role of reactive oxygen species (ROS), especially *O2-, as mediators of the bystander effect. Endogenously generated ROS, due to activation of NAD(P)H oxidases, play a key role in the induction of DNA damage in bystander cells. All the observed effects in bystander cells, such as alterations in gene expression patterns, chromosomal aberrations, sister chromatid exchanges, mutations, genome instability, and neoplastic transformation are the consequence of DNA damage.

Słowa kluczowe

EN

bystander effect; reactive oxygen species; ionising radiation; DNA repair; signalling pathways

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2003
• Tom: Vol. 48, nr 3
• Strony: 113--120
• Opis fizyczny: Bibliogr. 49 poz., rys.

Twórcy

autor

Szumiel I.

• Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, 16 Dorodna Str., 03-195 Warsaw, Poland, Tel./Fax: [phone],

Bibliografia

• 1. Azzam EI, de Toledo SM, Little JB (2001) Direct evidence for the participation of gap junction-mediated inte rcellular communication in the transmission of damage signals from alpha-particle irradiated to nonirradiated cells. Proc Natl Acad Sci USA 98:473−478
• 2. Azzam EI, de Toledo SM, Spitz DR, Little JB (2002) Oxidative metabolism modulates signal transduction and micronucleus formation in bystander cells from α-particleirradiated normal human fibroblast cultures. Cancer Res 62:5436−5442
• 3. Babior BM (1999) NADPH oxidase: an update. Blood 93:1464−1476
• 4. Barcellos-Hoff MH, Brooks AL (2001) Extracellular signaling through the microenvironment: a hypothesis relating carcinogenesis, bystander effects, and genomic instability. Radiat Res 156:618−627
• 5. Bishayee A, Hill HZ, Stein D, Rao DV, Howell RW (2001) Free radical-initiated and gap junction-mediated bystander effect due to nonuniform distribution of incorporated radioactivity in a three-dimensional tissue culture model. Radiat Res 155:335−344
• 6. Folkard M, Schettino G, Vojnovic B et al. (2001) A focused ultrasoft x-ray microbeam for targeting cells individually with submicrometer accuracy. Radiat Res 156:796−804
• 7. Goldberg Z, Lehnert BE (2002) Radiation-induced effects in unirradiated cells: a review and implications in cancer. Int J Oncol 21:337−349
• 8. Hashimoto M, Donald CD, Yannone SM, Chen DJ, Roy R, Kow YW (2001) A possible role of Ku in mediating sequential repair of closely opposed lesions. J Biol Chem 276:12827−12831
• 9. Hei TK, Wu LJ, Liu SX, Vannais D, Waldren CA, RandersPehrson G (1997) Mutagenic effects of a single and an exact number of alpha particles in mammalian cells. Proc Natl Acad Sci USA 94:3765−3770
• 10. Huo L, Nagasawa H, Little JB (2001) HPRT mutants induced in bystander cells by very low fluences of alpha particles result primarily from point mutations. Radiat Res 156:521−525
• 11. Iyer R, Lehnert BE, Svensson R (2000) Factors underlying the cell growth-related bystander responses to alpha particles. Cancer Res 60:1290−1298
• 12. Li WG, Miller FJ Jr, Zhang HJ, Spitz DR, Oberley LW, Weintraub NL (2001) H(2)O(2)-induced O(2) production by a non-phagocytic NAD(P)H oxidase causes oxidant injury. J Biol Chem 276:29251−29256
• 13. Li WG, Stoll LL, Rice JB et al. (2003) Activation of NAD(P)H oxidase by lipid hydroperoxides: mechanism of oxidantmediated smooth muscle cytotoxicity. Free Radic Biol Med 34:937−946
• 14. Little JB, Azzam EI, de Toledo SM, Nagasawa H (2002) Bystander effects: intercellular transmission of radiation damage signals. Radiat Prot Dosim 99:159−162
• 15. Little JB, Nagasawa H, Li GC, Chen DJ (2003) Involvement of the nonhomologous end joining DNA repair pathway in the bystander effect for chromosomal aberrations. Radiat Res 159:262−267
• 16. Lorimore SA, Coates PJ, Scobie GE, Milne G, Wright EG (2001) Inflammatory-type responses after exposure to ionizing radiation in vivo: a mechanism for radiation-induced bystander effects? Oncogene 20:7085−7095
• 17. Lorimore SA, Wright EG (2003) Radiation-induced genomic instability and bystander effects: related inflammatory-type responses to radiation-induced stress and injury? A revie Int J Radiat Biol 79:15−25
• 18. Lyng FM, Seymour CB, Mothersill C (2002) Early events in the apoptotic cascade initiated in cells treated with medium from the progeny of irradiated cells. Radiat Prot Dosim 99:169−172
• 19. Matsumoto H, Hayashi S, Hatashita M et al. (2001) Induction of radioresistance by a nitric oxide-mediated bystander effect. Radiat Res 155:387−396
• 20. Morgan WF, Hartmann A, Limoli CL, Nagar S, Ponnaiya B (2002) Bystander effects in radiation-induced genomic instability. Mutat Res 504:91−100
• 21. Mothersill C, Seymour C (1997) Medium from irradiated human epithelial cells but not human fibroblasts reduces the clonogenic survival of unirradiated cells. Int J Radiat Biol 71:421−427
• 22. Mothersill C, Seymour CB (1998) Cell−cell contact during gamma irradiation is not required to induce a bystander effect in normal human keratinocytes: evidence for release during irradiation of a signal controlling survival into the medium. Radiat Res 149:256−262
• 23. Mothersill C, Seymour C (2001) Radiation-induced bystander effects: past history and future directions. Radiat Res 155:759−767
• 24. Mothersill C, Seymour CB, Joiner MC (2002) Relationship between radiation-induced low-dose hypersensitivity and the bystander effect. Radiat Res 157:526−532
• 25. Nagasawa H, Cremesti A, Kolesnick R, Fuks Z, Little JB (2002) Involvement of membrane signaling in the bystander effect in irradiated cells. Cancer Res 62:2531−2534
• 26. Nagasawa H, Huo L, Little JB (2003) Increased bystander mutagenic effect in DNA double-strand break repairdeficient mammalian cells. Int J Radiat Biol 79:35−41
• 27. Nagasawa H, Little JB (1992) Induction of sister chromatid exchanges by extremely low doses of alpha particles. Cancer Res 52:6394−6396
• 28. Nagasawa H, Little JB (1999): Unexpected sensitivity to the induction of mutations by very low doses of alpha-particle radiation: evidence for a bystander effect. Radiat Res 152:552−557
• 29. Nagasawa H, Little JB (2002) Bystander effect for chromosomal aberrations induced in wild-type and repair deficient CHO cells by low fluences of alpha particles. Mutat Res 508:121−129
• 30. Narayanan PK, Goodwin EH, Lehnert BE (1997) Alpha particles initiate biological production of superoxide anions and hydrogen peroxide in human cells. Cancer Res 57:3963−3971
• 31. Prise KM, Belyakov OV, Folkard M, Michael BD (1998) Studies of bystander effects in human fibroblasts using a charged particle microbeam. Int J Radiat Biol 74:793−798
• 32. Rudat V, Bachmann N, Kupper JH, Weber KJ (2001) Overexpression of the DNA-binding domain of poly(ADP-ribose) polymerase inhibits rejoining of ionizing radiation-induced DNA double-strand breaks. Int J Radiat Biol 77:303−307
• 33. Sawant SG, Randers-Pehrson G, Geard CR, Brenner DJ,Hall EJ (2001) The bystander effect in radiation oncogenesis: I. Transformation in C3H 10T1/2 cells in vitro can be initiated in the unirradiated neighbors of irradiated cells. Radiat Res 155:397−401
• 34. Sawant SG, Randers-Pehrson G, Metting NF, Hall EJ (2001) Adaptive response and the bystander effect induced by radiation in C3H 10T(1/2) cells in culture. Radiat Res156:177−180
• 35. Sawant SG, Zheng W, Hopkins KM, Randers-Pehrson G, Lieberman HB, Hall EJ (2002) The radiation-induced bystander effect for clonogenic survival. Radiat Res 157:361−364
• 36. Schettino G, Folkard M, Prise KM, Vojnovic B, Bowey AG, Michael BD (2001) Low-dose hypersensitivity in Chinese hamster V79 cells targeted with counted protons using a charged-particle microbeam. Radiat Res 156:526−534
• 37. Seymour CB, Mothersill C (2000) Relative contribution of bystander and targeted cell killing to the low-dose region of the radiation dose-response curve. Radiat Res 153:508−511
• 38. Shao C, Aoki M, Furusawa Y (2001) Medium-mediated bystander effects on HSG cells co-cultivated with cells irradiated by X-rays or a 290 MeV/m carbon beam. J Radiat Res 42:305−316
• 39. Shao C, Furusawa Y, Aoki M, Matsumoto H, Ando K (2002) Nitric oxide-mediated bystander effect induced by heavy-ions in human salivary gland tumour cells. Int J Radiat Biol 78:837−844
• 40. Szumiel I (1998) Monitoring and signalling of radiationinduced damage in mammalian cells. Radiat Res 150;S1:s92−s101
• 41. Szumiel I, Kapiszewska M, Kruszewski M, Iwanenko T, Lange CS (1995) Content of iron and copper in the nuclei and induction of pH 9-labile lesions in L5178Y sublines inversely cross-sensitive to H2O2 and X-rays. Radiat Environ Biophys 34:113−119
• 42. Thannickal VJ, Aldweib KD, Fanburg BL (1998) Tyrosine phosphorylation regulates H2O2 production in lung fibroblasts stimulated by transforming growth factor beta 1. J Biol Chem 273:23611−23615
• 43. Thannickal VJ, Day RM, Klinz SG, Bastien MC, Larios JM, Fanburg BL (2000) Ras-dependent and –independent regulation of reactive oxygen species by mitogenic growth factors and TGF-beta 1. FASEB J 14:1741−1748
• 44. Thannickal VJ, Fanburg BL (1995) Activation of an H2O2-generating NADH oxidase in human lung fibroblasts by transforming growth factor beta 1. J Biol Chem 270:30334−30338
• 45. Trott KR (2001) Non-targeted radiation effects in radiotherapy-roles of radiation-induced genomic instability and of the bystander effect in cancer cure by radiotherapy. Acta Oncol 40:976−980
• 46. Trucco C, Oliver FJ, de Murcia G, Menissier-de Murcia J (1998) DNA repair defect in poly(ADP-ribose) polymerasedeficient cell lines. Nucleic Acids Res 26:2644−2649
• 47. Watson GE, Lorimore SA, Macdonald DA, Wright EG (2000) Chromosomal instability in unirradiated cells induced in vivo by a bystander effect of ionizing radiation. Cancer Res 60:5608−5611
• 48. Zhou HN, Suzuki M, Geard CR, Hei TK (2002) Effects of irradiated medium with or without cells on bystander cell responses. Mutat Res − Fund Mol Mech Mut 499:135−141
• 49. Zhou H, Suzuki M, Randers-Pehrson G et al. (2001) Radiation risk to low fluences of alpha particles may be greater than we thought. Proc Natl Acad Sci USA 98:14410−14415 info.ps.gz