Synthesis, Biological, Immunological and Anticancer Properties of a New Brassinosteroid Ligand

• Autorzy: Swaczynova J. , Sisa M. , Hnilickova J. , Kohout J.
• Konferencja: 21st Conference on Isoprenoids, 23-29 September 2005, Białystok-Białowieża, Poland
• Języki publikacji: EN

Abstrakty

EN

We have developed polyclonal antibodies against the brassinosteroid, 24-epicastasterone. Antiserum against this substance was produced by immunizing rabbits and mice with 24-epicastasterone O-(carboxymethyl)oxime (24-epiCS-CMO) conjugated with bovine-serum albumin (BSA). The conjugates were prepared by a mixed anhydride procedure. The antibodies obtained were tested in enzyme-linked immunosorbent assay (ELISA) using 24-epiCS-CMO-peroxidase conjugate. The use of the ELISAallowed detection over the range of 0.01 to 500 pmoles. Natural brassinosteroids (BRs) like brassinolide, and 24-epibrassinolide exhibited relatively high cross-reactivities but many other natural BRs were inactive. The 24-epiCS-CMO ligand was also slightly active in second bean internode bioasaay and on cancer cell lines of different histopathological origin.

Słowa kluczowe

EN

brassinosteroids; phytohormones; antibodies; structure-activity relationship; cancer

• Wydawca: Polskie Towarzystwo Chemiczne
• Czasopismo: Polish Journal of Chemistry
• Rocznik: 2006
• Tom: Vol. 80, nr 4
• Strony: 629--635
• Opis fizyczny: Bibliogr. 28 poz.

Twórcy

autor

Swaczynova J.

autor

Sisa M.

autor

Hnilickova J.

autor

Kohout J.

• Laboratory of Growth Regulators, Palacky University and Institute of Experimental Botany ASCR, Šlechtitel? 11, 783 71 Olomouc, Czech Republic

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