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http://yadda.icm.edu.pl:443/baztech/element/bwmeta1.element.baztech-article-BUJ2-0002-0009

Czasopismo

Polish Journal of Chemistry

Tytuł artykułu

Inhibition of jack bean urease by a mixture of boric acid and phosphate buffer pH 6.96

Autorzy Krajewska, B.  Zaborska, W.  Leszko, M.  Brzózka, Z. 
Treść / Zawartość
Warianty tytułu
Języki publikacji EN
Abstrakty
EN Inhibition of jack bean urease by a mixture of boric acid and phosphate buffer pH 6.96 was studied. The inhibition constants of the mixture were determinated in solutions of phosphate buffer (22-155mM) by measuring the initial reaction rates for a series of boric acid concentrations (0-3 mM) at a series of substrate concentrations (2-50 mM urea). It was found that boric acid is a simple competitive inhibitor of urea se with the inhibition constant of the system changing from 0.23 mM in 22 mM phosphate buffer to 0.76 mM in 155 mM phosphate buffer. It was shown, that boric acid and phosphate buffer pH 6.96 are mutually exclusive competitive inhibitors of jack bean urease. The inhibition constants of boric acid and of phosphate buffer pH 6.96 in an inhibitor-free system were determinated to be 0.12 mM and 21 mM, respectively.
Słowa kluczowe
EN urease   boric acid   kinetics   inhibition   catalysis  
Wydawca Polskie Towarzystwo Chemiczne
Czasopismo Polish Journal of Chemistry
Rocznik 1999
Tom vol. 73 nr 2
Strony 359--366
Opis fizyczny
Twórcy
autor Krajewska, B.
autor Zaborska, W.
autor Leszko, M.
autor Brzózka, Z.
Bibliografia
Kolekcja BazTech
Identyfikator YADDA bwmeta1.element.baztech-article-BUJ2-0002-0009
Identyfikatory