Sol-Gel Immobilization of Aldehyde Dehydrogenase and NAD+ on Screen-Printed Electrodes for Designing of Amperometric Acetaldehyde Biosensor

• Autorzy: Noguer T. , Szydłowska D. , Marty J. , Trojanowicz M.
• Języki publikacji: EN

Abstrakty

EN

The stability of response of amperometric biosensors based on entrapment of aldehyde dehydrogenase (AlDH) and nicotinamide adenine dinucleotide (NAD+) in a sol-gel matrix on the surface of screen-printed electrodes modified with the mediator Meldola Blue-Reinecke salt was studied. The operational stability of biosensors was tested using acetaldehyde as substrate by applying a potential of -150 mV vs. pseudo Ag/AgCl screen-printed electrodes. The type and ratio of silicium alkoxides precursors, the amounts of AlDH and NAD+, as well as the influence of drying and storage temperature on the stability of electrodes were examined. Among the tested biosensors, the most stable devices were those incorporating a sol-gel layer containing both AlDH and NAD+, which was covered by an additional sol-gel layer in order to avoid the co-enzyme leaching.

Słowa kluczowe

EN

acetaldehyde; aldehyde dehydrogenase; NAD+; sol-gel entrapment; screen-printed electrode; Meldola Blue

• Wydawca: Polskie Towarzystwo Chemiczne
• Czasopismo: Polish Journal of Chemistry
• Rocznik: 2004
• Tom: Vol. 78 / nr 9
• Strony: 1679--1689
• Opis fizyczny: Bibliogr. 20 poz., rys.

Twórcy

autor

Noguer T.

• Université de Perpignan, Centre de Phytopharmacie, UMR CNRS 5054, 52, Avenue de Villeneuve, 66860 Perpignan Cedex, France

autor

Szydłowska D.

• Université de Perpignan, Centre de Phytopharmacie, UMR CNRS 5054, 52, Avenue de Villeneuve, 66860 Perpignan Cedex, France

autor

Marty J.

• Université de Perpignan, Centre de Phytopharmacie, UMR CNRS 5054, 52, Avenue de Villeneuve, 66860 Perpignan Cedex, France

autor

Trojanowicz M.

• Université de Perpignan, Centre de Phytopharmacie, UMR CNRS 5054, 52, Avenue de Villeneuve, 66860 Perpignan Cedex, France

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