Combined pH/Organic Modifier Double Gradient Reversed-Phase HPLC

• Autorzy: Kaliszan R. , Markuszewski M. , Wiczling P.
• Języki publikacji: EN

Abstrakty

EN

A new procedure of a combined pH and organic solvent double gradient reversed-phase high-performance liquid chromatography (RP HPLC) has been proposed. The procedure is technically feasible and theoretically proved. The method is demonstrated to outstandingly enhance separation of ionizable analytes. The approach consists in simultaneous developing of linear gradients of pH and of the organic modifier in the mobile phase. An extraordinary, systematically increasing eluting power of the mobile phase leads to a remarkable analyte peak compression, accompanied by a strongly reduced peak tailing.

Słowa kluczowe

EN

pH gradient/organic gradient HPLC; pKa; ionizable analytes

• Wydawca: Polskie Towarzystwo Chemiczne
• Czasopismo: Polish Journal of Chemistry
• Rocznik: 2004
• Tom: Vol. 78 / nr 8
• Strony: 1047--1056
• Opis fizyczny: Bibliogr. 19 poz., rys.

Twórcy

autor

Kaliszan R.

autor

Markuszewski M.

autor

Wiczling P.

• Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Gen. J. Hallera 107, 80-416 Gdańsk, Poland

Bibliografia

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