The Application of Qualitative Analysis of DNA Bulky Adducts in the Diagnoosis of Brain Tumours

• Autorzy: Barciszewska A. , Gawrońska I. , Żukiel R. , Nowak S. , Barciszewska M. Z.
• Języki publikacji: EN

Abstrakty

EN

Damage to DNA(base modification) is generally considered to be causative and directly related to tumour formation. Interaction of chemical carcinogens with DNA either directly or after metabolic activation, typically involves covalent binding of an electrophilic compound with a nucleophilic site in DNA. Guanine is by far the most prevalent target although adducts have been reported for all bases. We analysed the occurrence of bulky hydrophobic derivatives of DNA (adducts) in human brain tumour tissues. DNA was isolated, enzymatically digested to nucleotides and labeled with [_-32P]ATP and T4 polynucleotide kinase. Radioactive nucleotides were separated on anion-exchange polyethyleneimine cellulose (PEI) thin layer chromatography (TLC).We found that all brain tumours have similar DNA adducts pattern, although there are some significant differences for a particular disease. It turned out that DNA of a glioblastoma multiforme contains a rich array of modified bases in contrast to meningeoma tissues DNA, which shows only a few nucleotides. Location of spots on TLC of DNA adducts resemble a triangle, which is specific for brain tumours. This method of analysis may be very useful in classification and clinical diagnosis of brain tumours.

Słowa kluczowe

EN

DNA adducts; PEI-polyethyleneimine-cellulose; brain tumours; clinical diagnosis; DNA

• Wydawca: Polskie Towarzystwo Chemiczne
• Czasopismo: Polish Journal of Chemistry
• Rocznik: 2003
• Tom: Vol. 77 / nr 11
• Strony: 1607--1614
• Opis fizyczny: Bibliogr. 16 poz., rys.

Twórcy

autor

Barciszewska A.

• Department of Neurosurgery, Karol Marcinkowski University School of Medical Sciences, Przybyszewskiego 49, 60-355 Poznań, Poland
• Faculty of Chemistry, A. Mickiewicz University, Grunwaldzka 6, 60-780 Poznań, Poland

autor

Gawrońska I.

• Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Noskowskiego 12, 61-704 Poznań, Poland

autor

Żukiel R.

• Department of Neurosurgery, Karol Marcinkowski University School of Medical Sciences, Przybyszewskiego 49, 60-355 Poznań, Poland

autor

Nowak S.

• Department of Neurosurgery, Karol Marcinkowski University School of Medical Sciences, Przybyszewskiego 49, 60-355 Poznań, Poland

autor

Barciszewska M. Z.

• Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Noskowskiego 12, 61-704 Poznań, Poland

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