Structure of the Polysaccharide O-Antigen of Salmonella Aberdeen (O : 11)

• Autorzy: Szafranek J. , Kaczyńska M. , Kaczyński Z. , Gajdus J. , Czerwicka M. , Dziadziuszko H. , Głośnicka R.
• Języki publikacji: EN

Abstrakty

EN

O-specific polysaccharide (OPS) of Salmonella Aberdeen was obtained from bacterial cell mass by water-phenol extraction procedure of lipopolysaccharide (LPS) followed by its mild acid hydrolysis and gel filtration of soluble carbohydrate material. Rhamnose, galactose, N-acetyl-glucosamine and mannose were detected and their linkages were established. Sugar configurations, D or L, were determined for (S)-(+)-2-butyl glycosides on an achiral capillary column. The structure of OPS was determined by analysis of spectra of 1H and 13C NMR and homonuclear and heteronuclear correlations spectra. Anomeric configurations were tentatively assigned by chromium trioxide oxidation and later proved by anomeric proton chemical shifts, H1-H2 coupling constants and proton coupled 13C spectra. Sugar sequences were established from comparisons of specific carbon shifts with those from literature, two-dimensional nuclear Overhauser effect spectroscopy (NOESY) and heteronuclear multiple-bond correlation experiments (HMBC). The repeating unit of S. Aberdeen OPS has the structure: _3)-_-D-GlcpNAc-(1_3)-[ _-D-Manp-(1_4)-]_-D-Galp-(1_4)-_-L-Rhap-(1_

Słowa kluczowe

EN

Salmonella Aberdeen; O-antigen; repeating unit structure; NMR

• Wydawca: Polskie Towarzystwo Chemiczne
• Czasopismo: Polish Journal of Chemistry
• Rocznik: 2003
• Tom: Vol. 77 / nr 9
• Strony: 1135--1140
• Opis fizyczny: Bibliogr. 15 poz., rys.

Twórcy

autor

Szafranek J.

• Faculty of Chemistry, University of Gdañsk, 80-952 Gdańsk, Sobieskiego 18, Poland

autor

Kaczyńska M.

• Faculty of Chemistry, University of Gdańsk, 80-952 Gdańsk, Sobieskiego 18, Poland

autor

Kaczyński Z.

• Faculty of Chemistry, University of Gdańsk, 80-952 Gdańsk, Sobieskiego 18, Poland

autor

Gajdus J.

• Faculty of Chemistry, University of Gdańsk, 80-952 Gdańsk, Sobieskiego 18, Poland

autor

Czerwicka M.

• Faculty of Chemistry, University of Gdańsk, 80-952 Gdańsk, Sobieskiego 18, Poland

autor

Dziadziuszko H.

• Institute of Maritime and Tropical Medicine, 81-519 Gdynia, Powstania Styczniowego 9b, Poland

autor

Głośnicka R.

• Institute of Maritime and Tropical Medicine, 81-519 Gdynia, Powstania Styczniowego 9b, Poland

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