Tytuł artykułu
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Języki publikacji
Abstrakty
Two new analogues of trypsin inhibitor CMTI-III substituted with D-Arg or D-Lys in position 5 (P1) were synthesized by the solid-phase method. The first analogue ([D-Arg5] CMTI-III) displayed association equilibrium constants (Ka) with bovine -trypsin by about three orders of magnitude lower than did wild CMTI-III. The second analogue ([D-Lys5] CMTI-III) displayed Ka by about four orders of magnitude lower than [Lys5] CMTI-III. The configuration of basic amino acid residue (Arg or Lys) in the reactive site (position P1) of CMTI-III and its analogues played an important role for the stabilization of the inhibitors active structure.
Słowa kluczowe
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Czasopismo
Rocznik
Tom
Strony
519--523
Opis fizyczny
Bibliogr. 13 poz., rys.
Twórcy
autor
- Faculty of Chemistry, University of Gdańsk, 80-952 Gdańsk, Sobieskiego 18, Poland
autor
- Faculty of Chemistry, University of Gdańsk, 80-952 Gdańsk, Sobieskiego 18, Poland
autor
- Faculty of Chemistry, University of Gdańsk, 80-952 Gdańsk, Sobieskiego 18, Poland
Bibliografia
- 1. Kupryszewski G., Rolka K., Różycki J. and Ragnarsson U., Polish J. Chem., 68,879 (1994) and publications cited therein.
- 2. Kupryszewski G. and Rolka K., Cucurbita maxima trypsin inhibitor I and III (CMTI-I and III). Structure-activity relationship studies, in Hellenic Forum on Bioactive Peptides (RA Cordopatis ed.), Crete University Press, Heraklion 1997, p. 193 and publications cited therein.
- 3. Otlewski J., Krowarsch D. and Apostoluk W., Acta Biochim. Polon., 46, 531 (1999).
- 4. Jaśkiewicz A., Lis K., Różycki J., Kupryszewski G., Rolka K., Ragnarsson U., Zbyryt T. and Wilusz T., FEBS Lett., 436, 174 (1998).
- 5. Krowarch D., Dadlez M., Buczek O., Krokoszyńska I., Smalas A.O. and Otlewski J., J. .Mol. Biol., 289, 175 (1999).
- 6. Lee S.-L., Alexander R. S., Smollwood A., Trievel R., Marsinger L., Weber P.C. and Kettner C., Biochem., 36, 13180(1997).
- 7. Lesner A., Kupryszewski G. and Rolka K., Biochem. Biophys. Res. Commun., 285, 1350 (2001).
- 8. Sole N. A. and Barany G„ J. Org. Chem., 57, 5399 (1992).
- 9. Erlanger B.F., Kokowsky N. and Cohen W., Arch. Biochem. Biophys., 95, 271 (1961).
- 10. Empire M. and Laskowski M. Jr., Biochem., 21,227 (1982).
- 11. Park S. -J., Effect of amino acids replacment in ovomucoid third domains upon their association with serine proteinases. Ph.D. thesis, Purdue University, West Lafayette, IN, USA, 1985.
- 12. Otlewski J., Zbyryt T., Krokoszyńska I. and Wilusz T., Biol. Chem. Hoppe-Seyler, 371, 589 (1990).
- 13. Wieczorek M., Otlewski J., Cook J., Wilimowska-Pelc A., Wilusz T. and Laskowski M. Jr., Biochem. Biophys. Res. Commun., 126, 646 (1985)
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BUJ1-0020-0054