New Galanin Analogues with Contractile Activities on Rat Gastric Smooth MusclesR

Ruczyński, J.; Sawuła, A.; Konstański, Z.; Korolkiewicz, R.; Rekowski, P.

Artykuł - szczegóły

Tytuł artykułu

New Galanin Analogues with Contractile Activities on Rat Gastric Smooth MusclesR

Autorzy

Ruczyński J. , Sawuła A. , Konstański Z. , Korolkiewicz R. , Rekowski P.

Identyfikatory

Warianty tytułu

Języki publikacji

Abstrakty

We synthesized new 15-amino-acid-residue analogues of porcine galaninmodified in positions 2, 6, 8 or 14 and studied their activity on isolated rat gastric smooth muscles. Thus, we intended to characterize the molecular domains of GAL responsible for binding to its receptors and biological activity in the gastric fundus. All peptides were synthesized by the solid phase peptide synthesis with the use of Fmoc strategy. All galanin analogues contracted rat gastric fundus strips in a concentration-dependent manner with significantly increased or decreased activities as compared to GAL(1-15)NH2. As expected, the modifications introduced into the amino acid sequence of galanin caused changes in the interaction of GAL(1-15)NH2 with its receptors. Thus, residues: Trp2, Ser6, Gly8 and His14 in the amino acid sequence of GAL(1-15)NH2 play an important roles in the high-affinity binding of GAL to its receptors and biological activity in rat gastric smooth muscle cells.

Słowa kluczowe

galanin galanin analogues rat gastric smooth muscles

Wydawca

Polskie Towarzystwo Chemiczne

Czasopismo

Polish Journal of Chemistry

Rocznik

2002

Tom

Vol. 76 / nr 1

Strony

57--66

Opis fizyczny

Bibliogr. 36 poz., rys.

Twórcy

autor

Ruczyński J.

Faculty of Chemistry, University of Gdansk, Sobieskiego 18, 80-952 Gdansk, Poland

autor

Sawuła A.

Faculty of Chemistry, University of Gdansk, Sobieskiego 18, 80-952 Gdansk, Poland

autor

Konstański Z.

Department of Pharmacology, Medical University of Gdańsk Do Studzienki 38, 80-227 Gdańsk Poland

autor

Korolkiewicz R.

Department of Pharmacology, Medical University ofGdańsk Do Studzienki 37, 80-227 Gdańsk Poland

autor

Rekowski P.

Faculty of Chemistry, University of Gdansk, Sobieskiego 18, 80-952 Gdansk, Poland

Bibliografia

1. Tatemoto K., Rökaeus Ä., Jörnvall H., McDonald T.J. and Mutt V., FEBS Lett., 164, 124 (1983).
2. Kaplan L.M., Spindel E.R., Isselbacher K.J., and Chin W.W., Proc, Natl. Acad. Sci. USA 85 1065 (1988).
3. Rökaeus A. and Carlquist M., FEBS Lett., 234, 400 (1988).
4. Vrontakis M.E., Peden L.M., Duchworth M.L. and Friesen H.G., J. Biol. Chem., 262, 16755 (1987).
5. Norberg Ä., Sillard R, Carlquist M., Jörnvall H. and Mutt V., FEBS Lett., 288, 151 (1991).
6. Schmidt W.E., Kratzin H., Eckart K., Drevs D., Mundkowski G., Clemens A., Katsoulis S., Schafer H., Gallwitz B. and Creutzfeldt W., Proc. Natl. Acad. Sci. USA, 88, 11435 (1991).
7. Anglade l., Wang Y.X., Jensen J., Tramu G., Kah O. and Conlon J.M., J. Comp. Neurol, 350,63 (1994).
8. Langel Ü. and Bartfai T., Arm. N. Y. Acad. Sei., 863, 86 (1998).
9. Crawley J.N., Regul. Pept., 59, 1 (1995).
10. Bedecs K., Berthold M. and Bartfai T., Int. J. Biochem. Cell. Biol., 27, 337 (1997).
11. Bartfai T., Fisone G. and Langel Ü., TIPS, 13, 312 (1992).
12. Bartfai T. and Langel Ü., Ear. J. Med. Chem., 30, 163S (1995).
13. Branchek T. A, Smith K.E, Gerald C. and Walker M., Trends Pharm. Sci., 21, 109 (2000).
14. Kask K., Langel Ü. and Bartfai T., Cell. Mol. Neurobiol, 15, 653 (1995).
15. Ekblad E., Rökaeus A., Häkanson R. and Sundler F., Neuroscience, 16, 355 (1985).
16. Maggi C.A., Patacchini R., Santicioli P., Giuliani S., Turini D., Barbanti G., Beneforti B., Misuri D. and Meli A., Naunyn Schmiedebergs Arch. Pharmacol., 339, 415 (1989).
17. Ekblad E., AmbjömssonE., Ekman R., Häkanson R. and Sundler F., Dig. Dis. Sci., 34, 1217 (1989).
18. Fontaine J. and Lebrun P., Eur. J. Pharmacol., 164, 583 (1989).
19. Kuwahara A., Ozaki T. and Yanaihara N., Eur. J. Pharmacol., 164, 175 (1989).
20. Tamura K., Palmer J. M. and Wood J. D., Eur. J. Pharmacol., 136, 445 (1987).
21. Ekblad E., Häkanson R., Sundier F. and Wahlstedt C., Br. J. Pharmacol., 86, 24! (1985).
22. Fox J.E.T., Me Donald T.J., Kostolanska F. and Tatemoto K., Life Sci., 39, 103 (1986).
23. Maggi C. A., Santicioli P., Patacchini R., Turini D„ Barbanti G., Beneforti B., Giuliani S. and Meli A Eur. J. Pharmacol., 143, 135 (1987).
24. Ahtaridis S.A., Katoch S.S. and Moreland R.S., Am. J. Physiol., 274, G306 (1998).
25. Korolkiewicz R., Konstanski Z., Rekowski P., Ruczyński J„ Szyk A., Szczepańska R., Korolkiewicz K. Z. and Petrusewicz Pharm. Pharmacol. Commun., 6, 415 (2000).
26. Land T., Langel Ü., Löw M., Berthold M., Unden A. and Bartfai T., Int J. Pept. Protein Res 38 267 (1991) . ’
27. Yanaihara N., Mohizuki T., Takatsuka N., Iguchi K., Sato K., Kakuyama H., Li M. and Yanaihara C. Regul. Pept., 46, 93 (1993).
28. Jurćus A., Langel Ü. and Bartfai T., J. Peptide. Res., 49, 195(1997),
29. Pooga M., Jureus A., Rezael K., Hasanvan H., Saar K., Kask K., Kjellen P., Land T„ Halonen J., Mäeorg U., Uri A., Solyom S., Bartfai T. and Langel Ü., J. Peptide. Res., 51, 65 (1998).
30. Carpenter K.A., Schmidt R., Yue S. Y., Hodzic L., Pou C., Payza K., Godbout C., Brown W. and Roberts E Biochem., 38, 15295 (1999).
31. Chan WC and White PD: Fmoc Solid Phase Peptide Synthesis: A Practical Approach. Oxford, Oxford University Press, 2000.
32. Vane J.R., Br. J. Pharmacol, 12, 344 (1957).
33. Katsoulis S., Schmidt W.E., Schworer H. and Creutzfeldt W., Br. J. Pharmacol., 101, 297 (1990).
34. Śliwiński W., Korolkiewicz R., Rekowski P., Halama A. and Korolkiewicz K.Z., Fundam. Clin. Pharmacol, 10,442(1996).
35. Ruczyński J., Szyk A., Konstański Z., Korolkiewicz R. and Rekowski P., Polish. J. Chem., 74, 1375 (2000).
36. Korolkiewicz R., Konstański Z., Rekowski P., Ruczyński J., Szyk A., Grzybowska M., Korolkiewicz K.Z. and Petrusewicz J., J. Physiol. Pharmacol., 52(1), 127 (2001

Typ dokumentu

Bibliografia

Identyfikator YADDA

bwmeta1.element.baztech-article-BUJ1-0020-0007