Wpływ bakterii ryzosferowych na zmianę liczebności drobnoustrojów w rędzinie wapiennej świeżo skażonej mieszaniną wielopierścieniowych węglowodorów aromatycznych z olejem napędowym

• Autorzy: Gałązka A. , Król M. , Perzyński A.

Warianty tytułu

EN

Effect of rhizosphere bacteria on number of microorganisms in rendzina freshly polluted with mixture of polycyclic aromatic hydrocarbons (PAH'S) and diesel fuel (DF)

• Języki publikacji: PL

Abstrakty

PL

Przedstawiono badania wpływu ochronnego szczepów Azospirillum spp. i Pseudomonas stutzeri na liczebność mikrobiologiczną w rędzinie wapiennej świeżo skażonej mieszaniną WWA i ON. Po zastosowaniu szczepienia roślin stwierdzono statystycznie istotny wzrost liczby drobnoustrojów glebowych (ogólnej liczby bakterii, promieniowców, bakterii amonifikacyjnych, proteolitycznych, celulolitycznych, rozkładających fosforany oraz kopiotrofów i oligotrofów) oraz statystycznie znaczny spadek ogólnej liczby grzybów. Stwierdzono ponadto stymulujący wpływ oleju napędowego w dawkach O,1 i 1 % na wzrost liczby wymienionych drobnoustrojów.

EN

Rhizodegradation is the breakdown of organic contaminants in soil by soil dwelling microbes which is enhanced by the rhizospheres presence. The soil used in the experiment was collected from 0-30 cm layer in the uncontaminated area of agricultural use (Mięćmierz, Kazimierz Dolny, Poland). Pot experiment was conducted to evaluate the number of microorganisms in rendzina freshly contaminated with PAH's, diesel fuel and mixtures of PAH's and diesel fuel using inoculation of meadow feascue with rhizospheric bacteria Azospirillum spp. and Pseudomonas stutzeri. The strains of Azospirillum spp. and Pseudomonas stutzeri were able to nitrogen fix and degrade PAH's as the only source of carbon and energy. Experiment was carried out as a chamber pot study. Plants had been cultivated for 30 days and then harvested. The total number of various bacteria (ammonifiying, proteolitic, able to degrade phosphorus, copiotrophic, oligotrophic), actinomycetes and fungi in soil before and after vegetation period of investigated soil were the basis for the determination of the effectiveness of the inoculation of plants with Azospirillum spp. and Pseudomonas stutzeri. This research has confirmed higher total number of microorganisms after increase on total number of fungi in rendzina after inoculation of plants with all doses of P AH' s: total number of bacteria, actinomycetes and lower number of fungi. Statistically important decrease of total number of different bacteria and actinomycetes and statistically important increase on total number of fungi in rendzina after grown of meadow feascus inoculated with Azospirillum spp. and Pseudomonas stutzeri were observed together with increase of pollution doses. Number of different bacteria and actinomycetes were statistically higher in soils polluted with diesel fuel at the doses 0, I and 1 %. The differences in concentration between the inoculated or non-inoculated soils indicate that the presence of plant roots, in addition to the period of time, contributes to grown and adaptation of Azospirillum spp. and Pseudomonas stutzeri to a soil freshly polluted with P AH' s and diesel fuel.

Słowa kluczowe

PL

skażenie gleby; węglowodór aromatyczny; związek wielopierścieniowy; olej napędowy; bioremediacja; bakterie ryzosferowe

EN

soil pollution; aromatic hydrocarbon; polycyclic aromatic hydrocarbon; diesel fuel; bioremediation; rhizosphere bacteria

• Wydawca: Oficyna Wydawnicza Politechniki Rzeszowskiej
• Czasopismo: Zeszyty Naukowe Politechniki Rzeszowskiej. Budownictwo i Inżynieria Środowiska
• Rocznik: 2009
• Tom: z. 56 [268]
• Strony: 35--47
• Opis fizyczny: Bibliogr. 24 poz., il.

Twórcy

autor

Gałązka A.

autor

Król M.

autor

Perzyński A.

• Instytut Uprawy, Nawożenia i Gleboznawstwa, Puławy

Bibliografia

• 1. Andersen T.A., Guthire E.A., Walton B.T.: Bioremediation in the rhizosphere: plant roots and associated microbes clean contaminates soil. Environ. Sci. Technol., 1993 (27), 2630-2636.
• 2. Atlas R.M.: Petroleum biodegradation and oil spili bioremediation. Mar. Pollut. Buli., 1995(31), 178-182.
• 3. Balba M.T., Al-Awadhi N., Al-Daher R.: Bioremediation of oil-contaminated soil: microbiological methods for feasibility assessment and field evaluation. Journal of Microbiol. Methods, 1998(32), 155-164.
• 4. Chen Y., Banks M.K., Schwab A.P.: Pyrene degradation in the rhizosphere of tall fescue (Festuca arundinaces) and switchgrass (Panicum virgatum L.). Environ. Sci. Tech., 2003(37), 5778-5782.
• 5. Corgiě S.C., Beguiristain T., Leyval C.: Spatial distribution of bacterial communities and phenanthrene degradation in the rhizosphere of Lolium perenne L. Appl. Environ. Microbiol., 2004(70), 3552-3557.
• 6. Fan S., Li P., Gong Z., Ren W., He N.: Promotion of pyrene degradation in rhizosphere of alfalfa (Medicago sativa L.). Chemosphere, 2008(71), 1593-1598.
• 7. Gałązka A.: Effect of Azospirillum spp. and Pseudomonas stutzeri inoculation of plants on bioremediation of polycyclic aromatic hydrocarbons (in polish). IUNG-PIB, Puławy 2008.
• 8. Gogoi B.K., Dutta N.N., Goswami P., Mohan T.R.K.: A case study of bioremediation of petroleum-hydrocarbon contaminated soil at a crude oil spili. Adv. Environ. Res, 2003(70), 757-782.
• 9. Hattori R., Hattori T.: Sensitivity to salts and organic compound of soil bacteria isolated on diluted media. J. Gen. Appl. Microbiol., 1980(26), 1-14.
• 10. Joner EJ. et al.: Rhizosphere effects on microbial community structure and dissipation and toxicity of polycyclic aromatic hydrocarbons (PAHs) in spiked soil. Environ. Sci. Technol., 2001(35), 2773-2777.
• 11. Król M.J.: Azospirillum - association bacteria free nitrogen fixing (in polish). Monografie i rozprawy naukowe, IUNG-PIB, Puławy 2006.
• 12. Król M.J., Perzyński A., Leśniak A.: Pseudomonas stutzeri - endorhizosphere's association bacterium(in polish). Postępy Nauk Roi., 2007(4»), 81-91.
• 13. Liste H.H., Prutz L: Plant performance, dioxygenase - expressing rhizosphere bacteria, and biodegradation of weathered hydrocarbons in contaminated soil. Chemosphere, 2006(62), 1411-1420.
• 14. Maliszewska-Kordybach B., Smreczak B., Klimowicz-Pawlas A., Janusauskaite D.: Ecotoxicological effect of phenanthrene on nitryfying bacteria in soils of different properties. J. Environ. Qual, 2007(36), 1635-1645.
• 15. Margesin R., Schinner F.: Monitoring of bioremediation by soil biological activities. Chemosphere, 2000(40), 339-346.
• 16. Martin J.P.: Use of acid, rose bengal and streptomycin in the plate method for estimating soil fungi. Soil Science, 1950(69), 215-232.
• 17. Muratova A. et al.: Plantrhizosphere-microflora association during phytoremediation of PAH-contaminated soil. Int. J. Phytoremediation, 2003(5), 137-151.
• 18. Nichols T.D., Wolf D.C., Rogers H.B., Beyrouty C.A., Reynolds C.M.: Rhizosphere microbial populations in contaminated soils. Water Air and Soil Pollution, 1997 (95), 165-178.
• 19. Rentz J.A., Alvarez P.J.J., Schnoor J.L.: Repression of Pseudomonas putida phenanthrene - degrading activity by plant root extracts and exudates. Environ. Microbiol., 2005(6), 574-583.
• 20. Rodina A.: Mikrobiologiczne metody badania wód. PWRiL, Warszawa 1968.
• 21. Santos E. et al.: Anthracene biodegradation and surface activity by an iron-stimulated Pseudomonas sp. Bioresource Technology, 2008(99), 2644-2649.
• 22. Strzelczyk E., Szpotański T.: Cellulolitic and pectoliytic activity of streptomycetes isolated from root-free soil, rhizosphere and mycorrhizosphere of pine (Pinus silvestris L.). Biol. Fertil. Soils., 1989(7), 365-369.
• 23. Wallace R., Lockhead A.: Qualitative studies of soil microorganisms. Aminoacid requirements of rhizosphere bacteria. Can. J. Research, section C, 1950(28), 1-6.
• 24. Zhuang X., Chen J., Shim H., Bai Z.: New advances in plant growth-promoting rhizobacteria for bioremediation. Environ. Intern., 2007(33), 406-413.