Rozróżnianie aminokwasów z wykorzystaniem elektronicznego języka

• Autorzy: Jańczyk M. , Wróblewski W. , Ciosek P.

Warianty tytułu

EN

Discrimination of amino acids by electronic tongue

• Języki publikacji: PL

Abstrakty

PL

Obecnie potrzebne są metody szybkiego, dokładnego, a przede wszystkim taniego oznaczania aminokwasów w próbkach biologicznych. Do takich technik należy potencjometryczny elektroniczny język, który pozwala na rozróżnianie próbek ciekłych. W pracy przedstawiono zastosowanie potencjometrycznego elektronicznego języka do klasyfikowania 5 aminokwasów. Sprawdzono możliwość rozpoznania poszczególnych analitów w roztworach o różnych pH, a następnie wykorzystując metodę częściowych najmniejszych kwadratów (PLS) rozróżniono poszczególne aminokwasy.

EN

In the last few years medical discoveries showed that presence of amino acids can be associated with many diseases. Usually, these compounds are determined using HPLC method (high performance liquid chromatography), however, due to its noxiousness, new methods are investigated. Application of electronic tongue system can be an alternative method for fast, accurate, affordable determination of amino acids in biological samples. This device is used for analysis and classification of complex liquid samples. Its main advantage is the ability to distinguish between samples containing various components on-line, without prior sample preparation and modification. In this paper there are presented preliminary studies on the recognition of 5 amino acids (glutamic acid, alanine, serine, arginine, aspartic acid) with use of a sensor array composed of ion-selective electrodes of various selectivity. Chemical images of the samples were obtained by measuring the electrode signals in solutions of amino acids for various pH ranging from 2.5 to 11.5. The possibility of identification of individual analytes in solutions of different pH was examined with use of the Partial Least Squares Analysis.

Słowa kluczowe

PL

elektroniczny język; aminokwasy; elektrody jonoselektywne; PLS

EN

electronic tongue; amino acids; ion-selective electrodes; PLS

• Wydawca: Wydawnictwo PAK
• Czasopismo: Pomiary Automatyka Kontrola
• Rocznik: 2010
• Tom: R. 56, nr 6
• Strony: 535--537
• Opis fizyczny: Bibliogr. 16 poz., rys., tab.

Twórcy

autor

Jańczyk M.

autor

Wróblewski W.

autor

Ciosek P.

• Politechnika Warszawska, Wydział Chemiczny, ul. Noakowskiego 3, 00-664 Warszawa,

Bibliografia

• [1] Ciosek P., Wróblewski W.: Miniaturized electronic tongue with an integrated reference microelectrode for the recognition of milk samples. Talanta. 76 2008. 548-556.
• [2] Dias L. A., Peres A. M., Veloso A. C. A., Reis F. S., Vilas-Boas M., Machado A. A. S. C.: An electornic tongue taste evaluation: Identification of goat milk adulteration with bovine milk. Sensors and Actuators B: Chemical. 136 2009. 209-217.
• [3] Rudnitskaya A., Polshin E., Kirsanov D., Lammertyn J., Nicolai B., Saison D., Delvaux F. R., Delvaux F., A. Legin: Instrumental measurement of beer taste attributes using an electronic tongue. Analytica Chimica Acta. 646 2009. 111-118.
• [4] Rudnitskaya A., Delgadillo I., Legin A., Rocha S. M., Costa A. -M., Simoes T.: Prediction of the Port wine age using an electronic tongue. Chemiometrics and Intelligrnt Laboratory Systems. 88 2007. 125-131.
• [5] Codinachs L. M., Kloock J. P., Schoning M. J., Baldi A., Ipatov A., Bratov A., Jimenez-Jorguera C.: Electronic integrated multisensor tongue applied to grape juice and wine analysis. The Analyst. 133 2008. 1440-1448.
• [6] Chen Q., Zhao J., Vittayapadung S.: Identification of the green tea grade level using electronic tongue and pattern recognition. Food Research International. 41 2008. 500-504.
• [7] Wei Z., Wang J., Liao W.: Technique potential for classification of honey by electronic tongue. Journal of Food Engineering. 94 2009. 260-266.
• [8] Kantor D. B., Hitka G., Fekete A., Balla C.: Electronic tongue for sensing taste changes with apricots during storage. Sensors and Actuators B. 131 2008. 43-47.
• [9] Beullens K., Meszaros P., Vermeir S., Kirsanov D., Legin A., Buysens S., Cap N., Nicolai B. M., Lammertyn J.: Analysis of tomato taste using two types of electronic tongues. Sensors and Actuators B. 131 2008. 10-17.
• [10] Kuhlman G., Keymeulen D., Buehler M., Kounaves S.: Detecting heavy metals in solution using electronic tongue 3-REDOX water quality sensors. IEEE Aerospace Confefence Proceedings. 1 2004. 363-377.
• [11] Rudnitskaya A., Ehlert A., Legin A., Vlasov Y., Buttgenbach S.: Multisensor system on the basis of an array of non-specific chemical sensors and artifical neutral networks for determination of inorganic pollutant in a model groundwater. Talanta. 55 2001. 425-431.
• [12] Ciosek P., Pokorska B., Romanowska E., Wróblewski W.: The recognition of growth conditions and metabolic type of plants by a potentiometric electronic tongue. Electroanalysis. 18 2006. 1266-1272.
• [13] Li L., Naini V., S. Ahmed U.: Utilization of a Modified Special-Cubic Design and an Electronic Tongue for Bitterness Masking Formulation Optimisation. Journal of pharmaceutical sciences. 96 10 2007. 2723-2734.
• [14] Legin A., Rudnitskaya A., Clapham D., Seleznev B., Lord K., Vlasov Y.: Electronic tongue for pharmaceutical analytics: quantification of tastes and masking effects. Analytical and Bioanalytical Chemistry. 380 2004. 36-45.
• [15] Lorenz J. K., Reo J. P., Hendl O., Worthington J. H., Petrossian V. D.: Evaluation of a taste sensor instrument (elec-tronic tongue) for use in formulation development. International Journal of Pharmaceutics. 367 2009. 65-72.
• [16] Zheng J. Y., Keeney M. P.: Taste masking analysis in pharmaceutical formulation development using an electronic tongue. International Journal of Pharmaceutics. 310 2006. 118-124.