A biochemical model for characterising the surface-active phospolipid bilayer of articular cartilage relative to acid-base equilibrium

• Autorzy: Pawlak Z. , Kotynska J. , Figaszewski Z. , Gadomski A. , Gudaniec A. , Oloyede A.
• Języki publikacji: PL

Abstrakty

EN

Purpose: This paper, addresses the question of how changes in acid - base equilibrium influence change in the charge density of the phospholipid bilayer on articular cartilage surfaces during lubrication. Design/methodology/approach: Liposomes have been used to mimic biological phospholipid membranes on articular cartilage surface where proteins are bounded, ions are transported, energy is transducted, and cellular processes take place. The charge density of the membrane was determined as a function of pH and electrolyte concentration from the microelectrophoretic method. Liposome membrane was prepared as an aqueous solution of NaCl under various pH conditions. Microelectrophoresis was used to examine the local acid-base equilibrium of the electrolytes with the membrane surface, which can be considered to model the phospholipids interface in articular cartilage. Findings: The adsorbed ions (H+, OH-, Na+, Cl-) which are present in the electrically charged solutions of liposome membrane comprising phosphatidycholine (PC), were found to exhibit pH-responsive quasi-periodic behavior. Research limitations/implications: We have established that the acid-base dissociation behavior in phospholipid bilayers of articular cartilage is a key to understanding biolubrication processes. For example,previous investigators found that the formation of the multilayer of polyisopeptide/hyaluronic acid depends on surface properties such as film thickness, surface friction, surface wetability; wetness and swelling behavior. Future work should consider the adsorption of polyelectrolyte ions, e.g., the glycoprotein lubricin and hyaluronan, on the liposome membrane surface in the presence of H+ and OH- ions. Originality/value: A novel model of the joints’ phospholipid bilayers has been created using liposome membrane This model can be applied in the investigation of polyelectrolyte ions such as lubricin, in articular cartilage. We have demonstrated that the acid-base processes on charged surfaces is a key mechanism in facilitating lubrication in human joints.

Słowa kluczowe

PL

biomateriały; chrząstka stawowa; własności elektryczne; fosfolipidy membranowe; pH

EN

biomaterials; electrical properties; lubrication; articular cartilage; surface charge density; phospholipids membrane; pH

• Wydawca: International OCSCO World Press
• Czasopismo: Archives of Materials Science and Engineering
• Rocznik: 2008
• Tom: Vol. 29, nr 1
• Strony: 24--29
• Opis fizyczny: Bibliogr. 39 poz.

Twórcy

autor

Pawlak Z.

autor

Kotynska J.

autor

Figaszewski Z.

autor

Gadomski A.

autor

Gudaniec A.

autor

Oloyede A.

• University of Technology and Life Sciences, ul. Seminaryjna 3, 85-326 Bydgoszcz, Poland,

Bibliografia

• [1] S. Mall, R.P. Sharma, J.M. East and A.G. Lee, Lipid-protein interaction in the membrane: studies with model peptide, Faraday Discussions 111 (1998) 127-136.
• [2] M. Benz, N Chen, G. Jay, J. Israelachvili, Static forces, structure and flow properties of complex fluids in highly confined geometries, Annals of Biomedical Engineering 33 (2005) 39-51.
• [3] B. Zappone, M. Ruths, G.W. Greene, G.D. Jay, J.N Israelachvili, Adsorption, Lubrication, and wear of lubricin on model surfaces, polymer brush-like behavior of a glycoprotein, Biophysical Journal 92 (2007) 1693-1706.
• [4] B.J. Hamrock, S.R Schmid, Bo.O. Jacobson, Fundamental of fluid film lubrication, Marcel Dekker, 2004.
• [5] I. Pasquali-Ronchetti, D. Quaglino, G. Mori, B. Bacchelli, P. Ghosh, Hyaluronan - phospholipid interactions, Journal of Structural Biology 120 (1997) 1-10.
• [6] B.A. Hills, Oliglamellar Lubrication of joints by surface active phospholipid, Journal of Rheumathology 16 (1989) 82-91.
• [7] B.A. Hills, R.W. Crawford, Normal and prostatic synovial joints are lubricated by surface-active phospholipid, Journal of Arthoplasty 18 (2003) 499-505.
• [8] T. Little, Fremann, S.A.V. Swanson, Experiments on friction in the human hip joint. In, V. Wright (ed). Lubrication and Wear in Joints, Sector, London, 1969.
• [9] J.L. Rabinowitz, J.R. Gregg, J.E. Nixon, Lipid composition of the tissues of human knee joints. II. Synovial fluid in trauma, Clinical Orthopedics Related Research 190 (1984) 292-298.
• [10] A.V. Sarma G.L. Powell, M. LaBerge, Phospholipids composition of articular cartilage boundary lubricant, Journal of Orthopedics Research 19 (2001) 671-675.
• [11] M. Benz, N.H. Chen, J.N. Israelachvili, Lubrication and wear properties of grafted electrolytes, hyaluronan and hylan, measured in the surface forces apparatus, Journal of Biomedical Material Research 71A (2004) 6-15.
• [12] G.D. Jay, D.A. Harris, C.J. Cha, Boundary lubrication by lubricin is mediated by O-linked P (1-3) Gal-GalNAc oligosaccharides, Glycoconjugate Journal 18 (2003) 807-815.
• [13] A. Oloyede, N.D. Broom, The generalized consolidation of articular cartilage-An investigation of its near-physiological response to static load, Connective Tissue Research 31/1 (1994) 75-86.
• [14] J.M. Prausnitz, Thermodynamics of structured fluids. Hard science for soft materials, Pure Applied Chemistry 72 (2000) 1819-1834.
• [15] Z. Pawlak, R.W. Crawford, A. Oloyede, Hypothetical model of hydrophilic lubrication in synovial joints, Proceedings of the International Tribology Conference, Austrib 2006, Brisbane, Australia, 2006, 1-6.
• [16] R.A. Stockwell, Lipid content of human costal and articular cartilage, Annals of Rheumatology Discussion 26 (1967) 481-486.
• [17] R.A. Freitas Jr., Nanomedicine, Volume I: Basic Capabilities, Landes Bioscience, Georgetown, IX, 1999.
• [18] V. Crescenzi, A. Taglienti, I. Pasquali-Ronchetti, Supramolecular structures prevailing in aqueous hyaluronic acid and phospholipids vesicles mixture: and electron microscopy and rhometric study, Colloids Surfaces A 245 (2004) 133-135.
• [19] D.A. Swarm, F.H. Silver, H.S. Slayter, W. Stafford, E. Shore, The molecular structure and lubricating activity of lubricin isolated from bovine and human synovial fluids, Biochemistry Journal 225 (1985) 195-201.
• [20] E.L. Radin, D.A. Swann and P.A. Weisser, Separation of a hyaluronate-free lubricating fraction from synovial fluid, Nature 228 (1970) 377-378.
• [21] A.M. Homola, J.N. Israelachvili, M.L. Gee, P.M McGuiggan, Measurements of an relation between the adhesion and friction of two surfaces separated by molecularly thin liquid films, Journal of Tribology 111 (1989) 675-682.
• [22] N.A. Cummings, G.L. Nordby, Measurements of synovial fluid pH in normal and arthritic knees, Arthritis Rheumatology 9 (1966) 47-56.
• [23] A. Berman, C. Drummond, J.N. Israelachvilli, Amontons' low at the molecular level, Tribology International 4 (1998) 95-101.
• [24] I. Goldie, A. Nachemson, Synovial pH in rheumatoid kneejoints. 1. The effect of synovectomy, Acta Orthopedic Scandinavica 40 (1969) 634-641.
• [25] T. Kitano, H. Ohashi, Y. Kadoja, A. Kabayshi, Y. Yutaniand Y. Yamano, Measurements of zeta potentials of particulate biomaterials in protein-rich hyaluron solution with changes in pH and protein constituents, Journal of Biomedical Materials Research A42 (1998) 453-457.
• [26] S.E. Burke, C.J. Barttel, pH-responses properties of multilayered poly(L-lysine)/hyaluronic acid surfaces, Biomacromolecules 4 (2003) 1773-1783.
• [27] S.E Burke, C.J. Barrett, Acid-base interaction of a weak polyelectrolytes in multilayer thin films, Langmuir 19 (2003) 3297-3303.
• [28] L. Kotynska, Z.A. Figaszewski, Adsorption equilibria between liposome membrane formed of phosphatidylcholine and aqueous sodium chloride solution as a function of pH, Biochemica, Biophysica Acta 1720 (2005) 22-27.
• [29] C. Huang, Studies on phosphatidylcholine vesicles. Formation and physical characteristics, Biochemistry 8 (1963) 344-352.
• [30] A. Gadomski, J. Siodmiak, A novel model of protein crystals growth: kinetic limits, length scales and the role of the double layer, Croatica Chemica Acta 76 (2003) 129-136.
• [31] A.E. Alexander, P. Johnson, Colloid Science, Clarendon Press, Oxford, 1949.
• [32] A. Gadomski, Description of the kinetics of a model tribopolymerization process, Journal Mathematical Chemistry 22 (1997) 161-183.
• [33] E. Czarnowska, T. Wierzchon, A. Maranda-Niedbala, Properties of the surface layers on titanium alloy and their biocompatibility in vitro tests, Journal of Materials Processing Technology 92-93 (1999) 190-194.
• [34] A. Prina Mello, M.A. Bari, P. J. Prendergast, A comparison of excimer laser etching and dry etching process for surface fabrication of biomaterials, Journal of Materials Processing Technology 124 (2002) 284-292.
• [35] D. Krupa, J. Baszkiewicz, J. W. Sobczak, A. Bilinski, A. Barcz, Modifying the properties of titanium surface with the aim of improving its bioactivity and corrosion resistance, Journal of Materials Processing Technology 143-144 (2003) 158-163.
• [36] R. Krishnan, M. Kopacz, G.A. Ateshian, Experimental verification of the role of interstitial fluid pressurization in cartilage lubrication, Journal of Orthopaedic Research 22 (2004) 565-570.
• [37] L.P. Ward, K.N. Strafford, T.P. Wilks, C. Subramanian, The role of refractory element based coatings on the tribological and biological behavior of orthopedic implants, Journal of Materials Processing Technology 56 (1996) 364-374.
• [38] W. Kajzer, M. Kaczmarek, J. Marciniak, Biomechanical analysis of stent-oesophagus system, Journal of Materials Processing Technology 162-163 (2005) 196-202.
• [39] Z. Pawlak, J. Kotynska, Z.A. Figaszewski, A. Oloyede, A. Gadomski, A. Gudaniec: Impact of the charge density of phospholipid bilayers on lubrication of articular cartilage surfaces, Journal of Achievements in Materials andManufacturing Engineering 23/1 (2007) 47-50.