Failure in applicability of INT in dehydrogenase activity analysis while presence of high nitrate concentration

• Autorzy: Gnida A. , Surmacz-Górska J.
• Języki publikacji: EN

Abstrakty

EN

The research was based on a determination of dehydrogenase activity (DA) of activated sludge operating under high ammonium load conditions. The dehydrogenase activity analysis was made using an INT assay where the tetrazolium salt (eg. INT) serves as an artificial acceptor of protons from respiration chain. In spite the widespread use of the test several analytical problems have appeared. One of the failures was a precipitation reaction between the tetrazolium salt INT and nitrates. In addition, there were problems with the determination of optimal concentrations of INT and the optimal incubation time and a decrease in INT formazan production over time.

PL

Przedstawione doświadczanie obejmuje oznaczanie aktywności dehydrogenaz osadu czynnego pracującego w warunkach znacznego obciążenia ładunkiem azotu amonowego. Do oznaczania aktywności dehydrogenaz użyto INT, sól tetrazolową stanowiącą sztuczny akceptor protonów w łańcuchu oddechowym. Mimo powszechnej stosowalności tego testu podczas oznaczeń napotkano na szereg problemów analitycznych. Zaobserwowano zachodzenie reakcji strącania pomiędzy solą tetrazolową INT a grupą azotanową. Ponadto wystąpiły problemy z wyznaczeniem optymalnego stężenia INT oraz optymalnego czasu inkubacji, a także stwierdzono ubytek w czasie powstałego formazanu INT.

Słowa kluczowe

EN

dehydrogenase; activated sludge; INT

PL

dehydrogenaza; osad czynny; INT

• Wydawca: Silesian University of Technology
• Czasopismo: Architecture Civil Engineering Environment
• Rocznik: 2012
• Tom: Vol. 5, no. 2
• Strony: 95--100
• Opis fizyczny: Bibliogr. 16 poz.

Twórcy

autor

Gnida A.

autor

Surmacz-Górska J.

• Environmental Biotechnology Department, Silesian University of Technology, Akademicka 2, 44-100 Gliwice, Poland,

Bibliografia

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