Influence of laminar shear stress on cytoskeleton and ICAM-1 expression of endothelial cells

• Autorzy: Muller S. , Sun R. , Legrand S. , Labrador V. , Wang X. , Stoltz J. F.
• Konferencja: Proceedings of the International Conference on Engineering Rheology ICER '99
• Języki publikacji: EN

Abstrakty

EN

The aim of this work was to study the morphological behavior and the surface adhesion molecules expression and localization of a human endothelial cell line subjected in vitro to a laminar flow in a parallel plate flow chamber, by a 3-D fluorescence microscopy and cytofluorimetry. At rest, endothelial cells showed an array of microfilament bundles of the actin fibers, and a peripheral distribution of ICAM-1 molecules. After shear stress (1 to 30 dyne/cm2, 1 to 24 hours), the stress fibers appeared and were oriented related to the flow direction but also to the shear. The ICAM-1 expression varied according to the shear stress characteristics and their distribution at the cell surface appeared also modified and related to the stress fibers formation.

Słowa kluczowe

EN

endothelial cells; shear stress; cytoskeleton; adhesion molecules; fluorescence microscopy

PL

biomechanika

• Wydawca: Oficyna Wydawnicza Uniwersytetu Zielonogórskiego
• Czasopismo: Applied Mechanics and Engineering
• Rocznik: 1999
• Tom: Vol. 4, no spec.
• Strony: 151--156
• Opis fizyczny: Bibliogr. 16 poz., rys.

Twórcy

autor

Muller S.

autor

Sun R.

autor

Legrand S.

autor

Labrador V.

autor

Wang X.

autor

Stoltz J. F.

• Angiohématologie-Hémorhéologie, UMR CNRS 7563 (LEMTA), Faculté de Médecine de Nancy, Plateau de Brabois, 54500 Vandoeuvre les Nancy, FRANCE