Some remarks on evaluating the quality of the multiple sequence alignment based on the BAliBASE benchmark

• Autorzy: Błażewicz J. , Formanowicz P. , Wojciechowski P.
• Języki publikacji: EN

Abstrakty

EN

BAliBASE is one of the most widely used benchmarks for multiple sequence alignment programs. The accuracy of alignment methods is measured by bali score-an application provided together with the database. The standard accuracy measures are the Sum of Pairs (SP) and the Total Column (TC). We have found that, for non-core block columns, results calculated by bali score are different from those obtained on the basis of the formal definitions of the measures. We do not claim that one of these measures is better than the other, but they are definitely different. Such a situation can be the source of confusion when alignments obtained using various methods are compared. Therefore, we propose a new nomenclature for the measures of the quality of multiple sequence alignments to distinguish which one was actually calculated. Moreover, we have found that the occurrence of a gap in some column in the first sequence of the reference alignment causes column discarding.

Słowa kluczowe

EN

multiple sequence alignment; reference alignment; alignment accuracy

PL

wielokrotne wyrównanie sekwencji; dostosowanie odniesienia; dokładność ustawienia

• Wydawca: Oficyna Wydawnicza Uniwersytetu Zielonogórskiego
• Czasopismo: International Journal of Applied Mathematics and Computer Science
• Rocznik: 2009
• Tom: Vol. 19, no 4
• Strony: 675--678
• Opis fizyczny: Bibliogr. 2 poz.

Twórcy

autor

Błażewicz J.

• Institute of Computing Science Poznań University of Technology, Piotrowo 2, 60-965 Poznań, Poland; Institute of Bioorganic Chemistry Polish Academy of Sciences, Noskowskiego 12/14, 61–714, Poznań, Poland

autor

Formanowicz P.

• Institute of Computing Science Poznań University of Technology, Piotrowo 2, 60-965 Poznań, Poland; Institute of Bioorganic Chemistry Polish Academy of Sciences, Noskowskiego 12/14, 61–714, Poznań, Poland

autor

Wojciechowski P.

• Institute of Computing Science Poznań University of Technology, Piotrowo 2, 60-965 Poznań, Poland

Bibliografia

• [1] Thompson, J. D., Koehl, P., Ripp, R. and Poch, O. (2005). Balibase 3.0: Latest developments of the multiple sequence alignment benchmark, PROTEINS: Structure, Function, and Bioinformatics 61(1): 127-136.
• [2] Thompson, J. D., Plewniak, F. and Poch, O. (1999). A comprehensive comparison of multiple sequence alignment programs, Nucleic Acids Research 27(13): 2682-2690.