A method of estimation of the cell doubling time on basis of the cell culture monitoring data

• Autorzy: Korzyńska A. , Zychowicz M.
• Języki publikacji: EN

Abstrakty

EN

Investigation with time-lapse microscopy of the clonal growth of stem cells provides to the analysis of image sequences, which document development of a single clone in constant time increments. The cell doubling time (T2) determines the dynamics of the cell culture development as average time taken for a cell to complete the cell cycle and is typically estimated with cytometric measurements within several days. However our monitoring of the cell culture lasts shorter, so the dependence of T2 from measurements available from image sequences has been found and applied to the collected data. The results are compared with the cell doubling time estimated and published for the cell line by the lab, in which it has been established.

Słowa kluczowe

EN

cell culture; cell doubling-time; time laps microscopy

PL

hodowla komórkowa; czas podwojenia komórek; czas wygaśnięcia; mikroskopia

• Wydawca: Nałęcz Institute of Biocybernetics and Biomedical Engineering of the Polish Academy of Sciences ; Elsevier
• Czasopismo: Biocybernetics and Biomedical Engineering
• Rocznik: 2008
• Tom: Vol. 28, no. 4
• Strony: 75--82
• Opis fizyczny: Bibliogr. 17 poz., rys.

Twórcy

autor

Korzyńska A.

autor

Zychowicz M.

• Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, ul. Ks. Trojdena 4, 02-109 Warsaw, Poland,

Bibliografia

• 1. Bużańska L., Jurga M., Stachowiak E.K., Stachowiak M.K., Domańska-Janik K.: Focus on Neural Stem Cells. Neural Stem-Like Cell Line Derived from a Nonhematopoietic Population of Humane Ubilical Cord Blood. Stem Cell and Development. 2006, 15, 391-406.
• 2. Stokłosowa S.: Hodowla komórek i tkanek. PWN, 2004.
• 3. Torricelli A., Bisiach M., Spinelli L., Ubezio P.: From Flow Cytometric BrdUrd Data to Cell Population Growth and Doubling Time. Cytometry. 1997, 29, 222-232.
• 4. Kvitko O.V., Koneva I.I., Sheiko Y.I., Anisovich M.V.: Hunting the mechanisms of self-renewal of immortal cell populations by means of real-time imaging of living cells. Cell Biology International 2005, 29, 1019-1024.
• 5. Begg, A. C., McNally N. J., Shrieve D.C., Karcher H.: A method to measure the duration of DNA synthesis and the potential doubling time from a single sample. Cytometry. 1985, 6, 620-626.
• 6. Boezeman J., Raymakers R., Vierwinden G. & Linssen P.: Automatic analysis of growth onset, growth rate and colony size of individual bone marrow progenitors. Cytometry. 1997, 28, 305-310.
• 7. White R.A., Terry N.H.A., Meistrich M.L., Calkins D.P.: Improved method for computing potential doubling time from flow cytometric data. Cytometry. 1990, 11, 314-317.
• 8. Yamaguchi M., Ohkusu M., Biswas S.K., Kawamoto S.: Cytological Study of Cell Cycle of the Pathogenenic Yeast Cryptococcus neoformans. Jpn. J. Med. Mycol. 2007, 48, 147-152.
• 9. White R.A.: A theory for the analysis of cell populations with noncycling S phase cells. J. Theor. Biol. 1991, 150, 201-214.
• 10. Zwicker J.I., Proffitt R.T., Reynolds C.P.: A microcomputer program for calculating cell population doubling time in vitro and in vivo. Cancer Chemotherapy and Pharmacology. 1995, 37, 3, 203-210.
• 11. Wheldon T.E.: Mathematical Models in Cancer Research. Adam Hilger, Bristol and Philadelphia 1977.
• 12. Alberts B., Bray D., Lewis J., Raff M., Roberts K., Watson J.D.: Molecular Biology of the Cell, third edition. Garland Publishing Inc., New York, London 1994.
• 13. Bużańska L., Machaj E.K., Zabłocka B. et al.: Human Cord Blood - Derived Cells Attain Neuronal and Glial Features in vitro. J. Cell Sci, 2002, 115, 2131-2138.
• 14. Warren G.S. and Fowler M.W.: Cell number and cell doubling times during the development of carrot embryoids in suspension culture. Cellular and Molecular Life Sciences (CMLS), 1978, 34, 3, 356-357.
• 15. Korzyńska A., Strojny W., Hoppe A., Wertheim D., Hoser P.: Segmentation of microscope images of living cells. Pattern Analysis and Applications. 2007, 10, 301-319.
• 16. Korzyńska A., Jurga M., Domańska-Janik K., Strojny W., Włoskowicz D.: Analysis of Stem Cell Clonal Growth, In M. Kurzyński, E. Puchała, M. Woźniak, A. Żołnierek (Eds.), Computer Recognition Systems, Advances in Soft Computing. Springer-Verlag Heidelberg 2005, 577-584.
• 17. Korzyńska A., Jurga M., Dzwigała M., Domańska-Janik K., Strojny W.: Behaviour of neural stem cells in sequentially monitored culture. Materiały Konferencyjne VIII Ogólnopolskiej Konferencji Neurochemicznej. Warszawa 2006.