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Tytuł artykułu

The antibiotic induction of apoptotic - like changes in bacteria E. coli - GFP encapsulated in hollow fibers

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Encapsulation of bacteria in a semipermeable polymer membrane gives a lot of technological possibilities. Our aim was to evaluate the performance of bacteria encapsulated in hollow fibers when treated with chosen antibiotic. The antibiotic application may cause release of biologically active substances for which production the bacteria may be genetically modified. The encapsulated in HF bacteria Escherichia coli transfected with pQE-GFP (green fluorescent protein) plasmid were incubated with addition of gentamycin or tetracycline. The encapsulated in hollow fibers E. coli culture with addition of tetracycline proves the tetracycline impact on the bacteria viability increasing the necrotic bacteria share. Polypropylene modified membranes allow to avoid permeation of the bacteria through the membrane wall. E. coli encapsulated in HF may be used in future, in systems releasing the therapeutic factor.
Twórcy
autor
autor
autor
  • Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, ul. Ks. Trojdena 4, 02-109 Warsaw, Poland, jkawiak@cmkp.edu.pl
Bibliografia
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  • 2. Shah N.P.: Probiotic bacteria: selective enumeration and survival in dairy foods. J. Dairy Sci. , 2000, 83, 894-907.
  • 3. Hou R.C., Lin M.Y., Wang M.M., Tzen J.T.: Increase of viability of entrapped cells of Lactobacillus delbrueckii ssp. bulgaricus in artificial sesame oil emulsions. J. Dairy Sci., 2003, 86, 424-428.
  • 4. Sultana K., Godward G., Reynolds N., Arumugaswamy R., Peiris P., Kailasapathy K.: Encapsulation of probiotic bacteria with alginate-starch and evaluation of survival in simulated gastrointestinal conditions and in yoghurt. Int. J. Food Microbiol., 2000, 62(1-2), 47-55.
  • 5. Chang T.M., Prakash S.: Procedures for microencapsulation of enzymes, cells and genetically engineered microorganisms. Molec. Biotechnol., 2001, 17(3), 249-260.
  • 6. Prakash S., Chang T.M.: Artificial cells microencapsulated genetically engineered E. coli DH5 cells for the lowering of plasma creatinine in vitro and in vivo. Artif. Cells, Blood Substitutes & Immobilization Biotechnology, 2000, 28(5), 397-408.
  • 7. Prakash S., Chang T.M.: In vitro and in vivo uric acid lowering by artificial cells containing microencapsulated genetically engineered E. coli DH5 cells. Int. J. Artif. Organs, 2000, 23(7), 429-35.
  • 8. Granicka L.H., Wdowiak M., Kosek A., Świeżewski S., Wasilewska D., Jankowska E., Weryński A., Kawiak J.: Survival analysis of Escherichia coli encapsulated in hollow fibre membrane in vitro & in vivo. Preliminary report. Cell Transplantation, 2005, 14, 323-330.
  • 9. Lewis A.L., Cunning Z.L., Goreish H.H., Kirkwood L.C., Tolhurst L.A., Stratford P.W.: Crosslinkable coatings from phosphorylcholine- based polymers. Biomaterials, 2001, 22(2), 99-111.
  • 10. Homma H., Nagaoka S., Mezawa S., Matsuyama T., Masuko E., Ban N., Watanabe N., Niitsu Y.: Bacterial adhesion on hydrophilic heparinized catheters with compared with adhesion on silicone catheters, in patients with malignant obstructive jaundice. J. Gastroenterol., 2006, 31(6), 836-843.
  • 11. Wang J., Huang N., Yang P., Leng Y., Sun H., Liu Z.Y., Chu P.K.: The effect of amorphous carbon films deposited on polyethylene terephalate on bacterial adhesion. Biomater., 2004, 25(16), 3163-3170.
  • 12. Emery B.E., Dixit R., Formby C.C., Biedlingmaier J.F.: The resistance of maxillofacial reconstruction plates to biofilm formation in vitro. Laryngoscope, 2003, 113(11), 1977-1982.
  • 13. Walker S.L., Hill J.E., Redman J.A., Elimelech M.: Influence of growth phase on adhesion kinetics of Escherichia coli D21g. Appl. Environ. Microbiol., 2005, 71(6), 3093-3099.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BPZ1-0045-0017
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