Biological applications of electron spin resonance methods - spin trapping and spin labelling

• Autorzy: Maliszewska-Mazur M.
• Języki publikacji: EN

Abstrakty

EN

In this paper, use of electron spin resonance methods in biological investigations and trends in their development are presented. The first method, spin trapping technique consists in using of spin traps, i.e. compounds that form a stable free radical by reacting with an unstable free radical. Hydroxyl radical generation by living human granulocytes stimulated by dihydralazine was determined by ESR using DMPO as a spin trapping reagent. The second method, spin labelling is an example of a "reporter group" technique. The effect of dihydralazine on membrane bilayer was found by spin-labelling technique with the use of 5-, 12-, 16-doxylstearate.

Słowa kluczowe

EN

electron spin resonance; spin trapping; spin labelling; free radicals in living cells; O2; OH'; dihydralazine

PL

rezonans elektronowy; wolny rodnik; komórka ożywiona

• Wydawca: Nałęcz Institute of Biocybernetics and Biomedical Engineering of the Polish Academy of Sciences ; Elsevier
• Czasopismo: Biocybernetics and Biomedical Engineering
• Rocznik: 2007
• Tom: Vol. 27, no. 4
• Strony: 85--97
• Opis fizyczny: Bibliogr. 47 poz., rys., wykr.

Twórcy

autor

Maliszewska-Mazur M.

• Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Trojdena 4, 02-109 Warsaw, Poland,

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