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Abstrakty
A mathematical model for the micro-dynamics of cultured cells measured with the ECIS (Electrical Cell-substrate Impedance Sensing) system that can separately evaluate the cell-to-cell distance and the cell-to-substrate distance is proposed. For wide applications of this method, mathematical models considering various types of cells and confluent conditions were constructed. Using ECIS TM, frequency the characteristics of 25 Hz to 60 kHz of impedance of HUVEC (human umbilical vein endothelial cells), BAEC (bovine aortic endothelial cells), in the pre-confluent condition and the full confluent condition of each cell were measured. A mathematical model of the micro-dynamics of the cultured cells measured with ECIS from 1 kHz to 10 kHz, which was the most interesting frequency range for the micro-dynamic analysis in the ECIS methods is proposed. The evaluation method of the cell-to-cell distance (A) and the cell-to-substrate distance (h) could be improved. In the application, we investigated the effect of the X-ray radiation exposure from 1 Gy to 100 Gy on the cultured cells BAEC using the ECIS system. Impedance changes could be confirmed by exposure to 100 Gy. The X-ray stimulation of 100 Gy resulted in a significant increase of the value of the cell-to-cell distance (A).
Wydawca
Czasopismo
Rocznik
Tom
Strony
227--236
Opis fizyczny
Bibliogr. 8 poz., rys., wykr.
Twórcy
autor
autor
autor
autor
autor
autor
autor
autor
- 2-5-1 Shikata-cyo Okayama, 700-8558 Japan, goda@md.okayama-u.ac.jp
Bibliografia
- 1. Giaever L, Keese R.C.: A morphological biosensor for mammalian cells; Nature, 1993,366, 591-592.
- 2. Giaever I., Keese R.C.: Micromotion of mammalian cells measured electrically, Proc. Natl. Acad. Sci. USA., 1991, 88, 7896-7900.
- 3. Alen B.M., Winter M., Kamath A., Blackwell K., Reyes G., Giaever I., Keese C., Shasby M.D.: Histamine alters endothelial barrier function at cell-cell and cell-matrix sites; AJP. 2000, 278, 888-898.
- 4. Goda N., Yamamoto Y., Kataoka N., Okuda H., Kajiya F : Evaluation of Cell Behavior Parameters Obtained from Electrical Cell-substrate Impedance Sensing (ECIS) by a Physical Model with an Insulator Board, Punched Holes and Electrode; Trans. Jap. Soc. Medical and Biological Engineering, 2004,42,187-192.
- 5. Goda N., Kataoka N., Shimizu J., Mohri S., Yamamoto Y, Okuda H., Kajiya F.: Evaluation of Micro-motion of Vascular Endothelial Cells in Electrical Cell-Substrate Impedance Sencing (ECIS) Method Using a Mathematical Model; J. Mechanics in Medicine and Biology, 2005, 5, 357-368.
- 6. Schwan P. H.: Electrical properties of tissue and cell suspensions, in Advances in Biological and Medical Physics, eds.; J. H. Lawrence and C. A. Tobias (Academic press in, New York), 1957, 147-290.
- 7. Grimnes S., Martinsen O.G.: Cole electrical model - A critique and an alternative; IEEE Trans. Biomed. Eng., 2005, 52, 132-135.
- 8. Kataoka N., Iwaki K., Hashimoto K., Mochizuki S,, Ogasawara Y, Sato M., Tsujioka K., Kajiya F: Measurements of endothelial cell-to-cell and cell-to-substrate gaps and micromechanical properties of endothelial cells during monocyte adhesion; Proc. Natl. Acad. Sci. USA., 2002, 99, 15638-15643.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BPZ1-0043-0026