Lab-on-a-chip for quality classification of pig oocytes

• Autorzy: Śniadek P. , Walczak R. , Dziuban J. , Jackowska M. , Antosik P. , Jaśkowski J. , Kempisty B.
• Języki publikacji: EN

Abstrakty

EN

The popular veterinarian method of qualitative selection of animals oocytes for artificial fertilization is based on morphological aspects of cells. Selection is done by the trained veterinarian specialists observing under a microscope oocytes "flowing" in phosphate buffered saline (PBS) buffer. This is unsatisfactory selection method, one of the weakest points of agriculture breading industry, because it introduces large personal factor and cannot be automated. In this paper, the novel microspectrometry method for quality assessment of pig oocytes isolated from follicles of various size is described. Pig oocytes were successfully classified according to existing classification. We used a specially designed microfluidic chip for measurements. The chip was constructed of glass and silicon with integrated optical fiber. This is the first attempt of the lab-on-a-chip based methodology of quality assessment of oocytes of breeding animals.

Słowa kluczowe

EN

lab-on-a-chip; microspectrometry; single cell analyses; oocyte

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Optica Applicata
• Rocznik: 2011
• Tom: Vol. 41, nr 2
• Strony: 417--422
• Opis fizyczny: Bibliogr. 6 poz.

Twórcy

autor

Śniadek P.

autor

Walczak R.

autor

Dziuban J.

autor

Jackowska M.

autor

Antosik P.

autor

Jaśkowski J.

autor

Kempisty B.

• Division of Microengineering and Photovoltaics, Faculty of Microsystem Electronics and Photonics, Wrocław University of Technology, Janiszewskiego 11/17, 50-372 Wrocław, Poland

Bibliografia

• [1] COTICCHIO G., SERENI E., SERRAO L., MAZZONE S., IADAROLA I., BORINI A., What criteria for the definition of oocyte quality?, Annals of the New York Academy of Sciences 1034, 2004, pp. 132–144.
• [2] MURAYAMA Y., CONSTANTINOU C.E., OMATA S., Micro-mechanical sensing platform for the characterization of the elastic properties of the ovum via uniaxial measurement, Journal of Biomechanics 37(1), 2004, pp. 67–72.
• [3] URBANSKI J.P., JOHNSON M.T., CRAIG D.D., POTTER D.L., GARDNER D.K., THORSEN T., Noninvasive metabolic profiling using microfluidics for analysis of single preimplantation embryos, Analytical Chemistry 80(17), 2008, pp. 6500–6507.
• [4] ZEGGARI R., WACOGNE B., PIERALLI C., ROUX C., GHARBI T., A full micro-fluidic system for single oocyte manipulation including an optical sensor for cell maturity estimation and fertilisation indication, Sensors and Actuators B 125(2), 2007, pp. 664–671.
• [5] TAGUCHI K., ATSUTA K., NAKATA T., KIEDA M., Levitation of a microscopic object using plural optical fibers, Optics Communications 176(1–3), 2000, pp. 43–47.
• [6] WONJAE CHOI, JI-SU KIM, DO-HYUN LEE, KYUNG-KWANG LEE, DEOG-BON KOO, JE-KYUN PARK, Dielectrophoretic oocyte selection chip for in vitro fertilization, Biomedical Microdevices 10(3), 2008, pp. 337–345.