Optoelectronic bacteria cells detection system

• Autorzy: Grzelka J. , Szczypiński R. , Pijanowska D. G. , Grodecki R. , Lesiński J. , Grabiec P. , Łysko J. M.
• Języki publikacji: EN

Abstrakty

EN

In general flow cytometry enables the characterization of individual cells/microparticles. When a sample is injected into a flow cytometer, the randomly distributed cells are ordered into a stream of single cells. Hence, they can be individually analyzed in the detection system. In this paper, an optoelectronic compact and portable system for bacteria cells detection is described. The system consists of PDMS (polydimethylsiloxane) microfluidic chip, a duralumin holder with microfluidic connectors - inlets/outlets, as well as optic fiber connectors and waveguides for laser diode and photodetectors (avalanche photodiode BPYP 59 or photomultiplier 9658B). The fluorochrome is excited by light beam of an appropriate wavelength coming from a light source, e.g., the laser diode. The excitation light passes through hydrodynamically focused cells in the middle of the channel. After optical filtering, the light emitted by the fluorochrome is detected by a photodetector. Electrical signals from the selective nanovoltmeter are amplified and collected by the computer electronic system. Since in the real sample bacteria cells are selectively labeled with the fluorophores or antibodies conjugated with fluorochrome, in our experiments to evaluate lower detection limits of the developed detection system, exemplary fluorescent dye solutions of sodium fluorescein were used as testing solutions.

Słowa kluczowe

EN

microfluidics; optoelectronic detection; bacteria detection

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Optica Applicata
• Rocznik: 2011
• Tom: Vol. 41, nr 2
• Strony: 403--408
• Opis fizyczny: Bibliogr. 4 poz.

Twórcy

autor

Grzelka J.

autor

Szczypiński R.

autor

Pijanowska D. G.

autor

Grodecki R.

autor

Lesiński J.

autor

Grabiec P.

autor

Łysko J. M.

• Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, ul. Ks. Trojdena 4, 02-109 Warszawa, Poland

Bibliografia

• [1] YU-JEN PAN, RUEY-JEN YANG, Fabrication of UV epoxy resin masters for the replication of PDMS-based microchips, Biomed Microdevices 9(4), 2007, pp. 555–563.
• [2] LUCAS N., SICHER P., BUTTGENBACH S., PENACHE C., BARTELS V., KLAGES C.-P., Flexible master structures of SU-8 for double-sided moulding of PDMS, Proceedings of the 5th Euspen International Conference, May 9–12, 2005, Montpellier, France, pp. 665–668.
• [3] SZCZYPIŃSKI R., PIJANOWSKA D.G., Technologia i zastosowanie poli(dimetylosiloksanu) – PDMS w mikroukładach analitycznych, Elektronika 11, 2008, pp. 249–253 (in Polish).
• [4] SHAPIRO H.M., Practical Flow Cytometry, 4th Edition, Wiley–Liss, 2003, p. 45.