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Optical properties of deposit of red blood cells suspended in dextran solutions

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The optical properties of the deposit of red blood cells (RBCs) formed during sedimentation have been investigated. The human blood was obtained from healthy donors. RBCs were suspended in isotonic saline containing Dextran 70 of concentrations 2, 4 and 6 g/dl. Hematocrit of the samples was adjusted to 40%. The intensity of the light scattered by the samples of the RBC suspensions as a function of time and altitude from the bottom of a sample was measured. The region occupied by forming and sedimenting aggregates and the region occupied by deposit of the cells can be found in the sample and the temporal dependence of position of the interface separating both regions, called the deposit formation curve, can be determined. This curve exhibits the growth phase and the packing phase. We have obtained the mean intensity of light scattered by the deposit as a function of time. This intensity exhibits a monotonous decay with time over the growth as well as the packing phase, what shows that packing of the deposit occurs in the same way in both phases. The dependence from time of the mean hematocrit of the deposit was determined. We have introduced an empirical expression describing this dependence. Finally, using this dependence, we have obtained the mean intensity of light scattered by the deposit as a function of hematocrit. We have shown that this intensity follows an exponential decay. The values of an optical parameter of the deposit were found from this dependence.
Słowa kluczowe
Czasopismo
Rocznik
Strony
177--185
Opis fizyczny
Bibliogr. 23 poz.
Twórcy
autor
  • Department of Biophysics, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, ul. Jagiellońska 13, 85-067 Bydgoszcz, Poland
Bibliografia
  • [1] STEINKE J.M., SHEPHERD A.P., Comparison of Mie theory and the light scattering of red blood cells, Applied Optics 27(19), 1988, pp. 4027–4033.
  • [2] HAMMER M., SCHWEITZER D., MICHEL B., THAMM E., KOLB A., Single scattering by red blood cells, Applied Optics 37(31), 1998, pp. 7410–7418.
  • [3] FRIEBEL M., ROGGAN A., MÜLLER G., MEINKE M., Determination of optical properties of human blood in the spectral range 250 to 1100 nm using Monte Carlo simulations with hematocrit-dependent effective scattering phase functions, Journal of Biomedical Optics 11(3), 2006, p. 034021.
  • [4] PRIEZZHEV A.V., RYABOSHAPKA O.M., FIRSOV N.N., SIRKO I.V., Aggregation and disaggregation of erythrocytes in whole blood: Study by backscattering technique, Journal of Biomedical Optics 4(1), 1999, pp. 76–84.
  • [5] TSINOPOULOS S.V., SELLOUNTOS E.J., POLYZOS D., Light scattering by aggregated red blood cells, Applied Optics 41(7), 2002, pp. 1408–1417.
  • [6] SHVARTSMAN L.D., FINE I., Optical transmission of blood: Effect of erythrocyte aggregation, IEEE Transactions on Biomedical Engineering 50(8), 2003, pp. 1026–1033.
  • [7] SINGH M., VATSALA T.M., Erythrocytes sedimentation profiles under gravitational field as determined by He-Ne laser. II. Influence of erythrocyte shape, Biorheology 19(1–2), 1982, pp. 165–173.
  • [8] SINGH M., JOSEPH K.P., Erythrocytes sedimentation profiles under gravitational field as determined by He-Ne laser. VII. Influence of dextrans, albumin and saline on cellular aggregation and sedimentation rate, Biorheology 24(1), 1987, pp. 53–61.
  • [9] MUTRYNOWSKA J., GRZEGORZEWSKI B., Optical analysis of red blood cell sediment formation, Biorheology 44(4), 2007, pp. 285–297. Optical properties of deposit of RBCs suspended in dextran solutions 185
  • [10] KERNICK D., JAY A.W.L., ROWLANDS S., Erythrocyte settling, Canadian Journal of Physiological Pharmacology 52(6), 1974, pp. 1167–1177.
  • [11] OKA S., A physical theory of erythrocyte sedimentation, Biorheology 22(4), 1985, pp. 315–321.
  • [12] HUNG W.T., COLLINGS A.F., LOW J., Studies of the sedimentation of human blood, Proceedings of the Xth International Congress on Rheology, 1988, Sydney, Australia, pp. 425–427.
  • [13] REUBEN A.J., SHANNON A.G., Some problems in the mathematical modelling of erythrocyte sedimentation, IMA Journal of Mathematics Applied in Medicine and Biology 7(3), 1990,pp. 145–156.
  • [14] WOODLAND N.B., CORDATOS K., HUNG W.T., REUBEN A., HOLLEY L., Erythrocyte sedimentation in columns and the significance of ESR, Biorheology 33(6), 1996, pp. 477–488.
  • [15] HOLLEY L., WOODLAND N., HUNG W.T., CORDATOS K., REUBEN A., Influence of fibrinogen and haematocrit on erythrocyte sedimentation kinetics, Biorheology 36(4), 1999, pp. 287–297.
  • [16] NEU B., MEISELMAN H.J., Depletion-mediated red blood cell aggregation in polymer solutions, Biophysical Journal 83(5), 2002, pp. 2482–2490.
  • [17] NEU B., WENBY R., MEISELMAN H.J., Effects of dextran molecular weight on red blood cell aggregation, Biophysical Journal 95(6), 2008, pp. 3059–3065.
  • [18] PRIBUSH A., MEYERSTEIN N., Methodological aspects of erythrocyte aggregation, Recent Patents on Anti-Cancer Drug Discovery 2(3), 2007, pp. 240–245.
  • [19] NASH G.B., WENBY R.B., SOWEMIMO-COKER S.O., MEISELMAN H.J., Influence of cellular properties on red cell aggregation, Clinical Hemorheology 7, 1987, pp. 93–108.
  • [20] BOYNARD M., LELIERE J.C., Size determination of red blood cell aggregates induced by dextran using ultrasound backscattering phenomenon, Biorheology 27(1), 1990, pp. 39–46.
  • [21] BARSHTEIN G., TAMIR I., YEDGAR S., Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation, European Biophysics Letter 27(2), 1998, pp. 177–181.
  • [22] TUCHIN V.V., XU X.Q., WANG R.K., Dynamic optical coherence tomography in studies of optical clearing, sedimentation, and aggregation of immersed blood, Applied Optics 41(1), 2002, pp. 258–271.
  • [23] XU X., WANG R.K., ELDER J.B., TUCHIN V.V., Effect of dextran-induced changes in refractive index and aggregation on optical properties of whole blood, Physics in Medicine and Biology 48(9), 2003, pp. 1205–1221.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BPW7-0012-0139
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