Thin protein LB films as functional components within biosensors

• Autorzy: Cabaj J. , Chyla A. , Sołoducho J.
• Języki publikacji: EN

Abstrakty

EN

Biomolecules can often be incorporated and immobilized into Langmuir-Blodgett (LB) films by using covalent immobilization of proteins with glutaraldehyde. Laccase from Cerrena Unicolor was incorporated into an LB film deposited on a glass substrate. The samples fabricated in such a way can be used for the detection of phenolic compounds. The function of enzyme immobilization was carried out by glutaraldehyde added to a film of stearic acid and laccase. The sensor sensitization was achieved by an amphiphilic N-alkyl-bis(thiophene)diphenylamine admixed into the film. The interlaced diphenylamine derivative was expected to facilitate the electron transfer thereby enhancing the sensor sensitivity.

Słowa kluczowe

EN

Langmuir-Blodgett films; laccase; diphenylamine derivatives; biosensor; AFM

• Wydawca: De Gruyter
• Czasopismo: Materials Science Poland
• Rocznik: 2009
• Tom: Vol. 27, No. 3
• Strony: 684--692
• Opis fizyczny: Bibliogr. 16 poz.

Twórcy

autor

Cabaj J.

autor

Chyla A.

autor

Sołoducho J.

• Faculty of Chemistry, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland

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