Potentiometric tungsten electrodes with enzymes immobilised by the sol-gel technique

• Autorzy: Przybyt M.
• Języki publikacji: EN

Abstrakty

EN

The application of sol-gel transitions in development of potentiometric enzyme electrodes with urease, glucose oxidase and tyrosinase is described. Urease and glucose oxidase electrodes showed features of typical potentiometric electrodes with the response modified by the change of pH. The slopes of calibration curves in semilogarithmic coordinates were lower than the Nernstian values and the dynamic ranges were narrow for both electrodes. The tyrosinase electrode shows response to mono- and o-diphenols. The response of this electrode is not connected with the pH changes. The mechanism of the response of the tyrosinase electrode is discussed; it is proposed that the potential changes reflect the redox state of the enzyme. The electrode responds to reactant concentration (phenol, catechol and some others) even at concentrations 210-6 M. Such a high sensitivity makes the electrode a promising candidate for application in detecting traces of phenols.

Słowa kluczowe

EN

sol-gel; potentiometric enzyme electrode; urease; tyrosinase; glucose oxidase

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Materials Science
• Rocznik: 2003
• Tom: Vol. 21, No. 4
• Strony: 417--429
• Opis fizyczny: Bibliogr. 34 poz.,

Twórcy

autor

Przybyt M.

• Institute of General Food Chemistry, Department of Biochemistry and Food Science, Technical University of Łódź, 90-924 Łódź, ul. Wólczańska 171/173, Poland

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