Fluorescence detection of biological objects with ultraviolet and visible light-emitting diodes

• Autorzy: Kurilcik N. , Vitta P. , Zukauskas A. , Gaska R. , Ramanavicius A. , Kausaite A. , Jursenas S.
• Języki publikacji: EN

Abstrakty

EN

Recent progress in fabrication of semiconductor light emitting diodes (LEDs) allows these devices to be used for excitation of fluorescence of aromatic amino acids and other biofluorophores. In our work, a deep-UV UVTOPTM LED (280 nm) developed by Sensor Electronic Technology, Inc., was used for fluorescence characterisation of natural protein fluorophores in enzyme glucose oxidase (GOx) and in Bacillus subtilus dry spores (B. subtilus). A longer-wavelength Nichia LED (375 nm) and high-power LuxeonTM LED (450 nm) were used for fluorescence detection of enzyme cofactors. Combined spectral and fluorescence lifetime measurements using selective LED excitation enabled us to recognise the impact of specific autofluorophores in complex biological systems. Inexpensive LED-based fluorescence detectors can be used in designing biosensors and detect-to-warn systems.

Słowa kluczowe

EN

semiconductor light emitting diodes (LEDs); UV LEDs; fluorescence lifetime; frequency-domain measurements; glucose oxidase; Bacillus subtilus spores

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Optica Applicata
• Rocznik: 2006
• Tom: Vol. 36, nr 2-3
• Strony: 193--198
• Opis fizyczny: Bibliogr. 8 poz.,

Twórcy

autor

Kurilcik N.

autor

Vitta P.

autor

Zukauskas A.

autor

Gaska R.

autor

Ramanavicius A.

autor

Kausaite A.

autor

Jursenas S.

• Institute of Materials Science and Applied Research Vilnius University, Lithuania

Bibliografia

• [1] UV Solid-State Light Emitters and Detectors, Proc. NATO ARW, Series II, Vol. 144, [Eds.] M.S. Shur, A. Žukauskas, Kluwer, Dordrecht 2004.
• [2] ŽUKAUSKAS A., SHUR M.S., GASKA R., Introduction to Solid-State Lighting, Wiley, New York 2002.
• [3] VITTA P., KURILČIK N., JURŠĖNAS S., ŽUKAUSKAS A., LUNEV A., BILENKO Y., ZHANG J., HU X., DENG J., KATONA T., GASKA R., Deep-ultraviolet light-emitting diodes for frequency domain measurements of fluorescence lifetime in basic biofluorophores, Applied Physics Letters 87(8), 2005, p. 084106.
• [4] ZHONG D., ZEWAIL A.H., Femtosecond dynamics of flavoproteins: charge separation and recombination in riboflavine (vitamin B2)-binding protein and in glucose oxidase enzyme, Proceedings of the National Academy of Sciences of the United States of America 98(21), 2001, pp. 11867–72.
• [5] RINUY J., BREVET P.F., GIRAULT H.H., Second harmonic generation of glucose oxidase at the air/water interface, Biophysical Journal 77(6), 1999, pp. 3350–5.
• [6] BARTLETT P.N., BOOTH S., CARUANA D.J., KILBURN J.D., SANTAMARIA C., Modification of glucose oxidase by the covalent attachment of a tetrathiafulvalene derivative, Analytical Chemistry 69(4), 1997, pp. 734–42.
• [7] LADOKHIN A.S., Fluorescence spectroscopy in peptide and protein analysis, [In] Encyclopedia of Analytical Chemistry, [Ed.] R.A. Meyers, Wiley, Chichester 2000, p. 5762.
• [8] LAKOWICZ J.R., Principles of Fluorescence Spectroscopy, Kluwer, Plenum, New York 1999.