Quantitative evaluation of 52 kD Ro/SS-A intracellular distribution in keratinocytes exposed to UVB radiation

• Autorzy: Woźniacka A. , Langner K. , McCauliffe D.P. , Sysa-Jędrzejowska A. , Langner M.
• Języki publikacji: EN

Abstrakty

EN

The intracellular location of the 52 kD Ro/SS-A autoantigen and its reaction upon stimuli are constantly subject to debate. One question still not resolved concerns the behavior of this protein following UVB irradiation. In order to monitor 52kD Ro/SS-A in time, the peptide was tagged with green fluorescence protein and over-expressed in keratinocytes. Evolution after UVB irradiation was observed using confocal fluorescence microscopy. By accepting a model that assumes the proportionality of pixel intensity to local GFP density, the distribution of the imaged protein in its relative density space can be calculated. Data analysis shows that the initial protein punctate structures located in the cytoplasm tend to disintegrate in a time-dependent manner. This agrees qualitatively with experimental evidence stating that the efficiency of immunological labeling is altered after UVB irradiation. Such dispersion must also co-exist with eventual enhanced exposure of 52 kD Ro/SS-A to the cell surface, which has been pointed out in the past by immunostaining experiments

Słowa kluczowe

EN

autoantibodies; green fluorescent protein; Ro 52 kD/SS-A; histogram

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Optica Applicata
• Rocznik: 2004
• Tom: Vol. 34, nr 1
• Strony: 77--85
• Opis fizyczny: Bibliogr. 21 poz., rys.

Twórcy

autor

Woźniacka A.

• Department of Dermatology, Medical University of Łódź, ul. Kremieniecka 5, 94-017 Łódź, Poland

autor

Langner K.

• Institute of Physics, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27,50-370 Wrocław, Poland

autor

McCauliffe D.P.

• Department of Dermatology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599-7287, USA

autor

Sysa-Jędrzejowska A.

• Department of Dermatology, Medical University of Łódź, ul. Kremieniecka 5, 94-017 Łódź, Poland

autor

Langner M.

• Institute of Physics, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27,50-370 Wrocław, Poland

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