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The intracellular location of the 52 kD Ro/SS-A autoantigen and its reaction upon stimuli are constantly subject to debate. One question still not resolved concerns the behavior of this protein following UVB irradiation. In order to monitor 52kD Ro/SS-A in time, the peptide was tagged with green fluorescence protein and over-expressed in keratinocytes. Evolution after UVB irradiation was observed using confocal fluorescence microscopy. By accepting a model that assumes the proportionality of pixel intensity to local GFP density, the distribution of the imaged protein in its relative density space can be calculated. Data analysis shows that the initial protein punctate structures located in the cytoplasm tend to disintegrate in a time-dependent manner. This agrees qualitatively with experimental evidence stating that the efficiency of immunological labeling is altered after UVB irradiation. Such dispersion must also co-exist with eventual enhanced exposure of 52 kD Ro/SS-A to the cell surface, which has been pointed out in the past by immunostaining experiments
Słowa kluczowe
Czasopismo
Rocznik
Tom
Strony
77--85
Opis fizyczny
Bibliogr. 21 poz., rys.
Twórcy
autor
- Department of Dermatology, Medical University of Łódź, ul. Kremieniecka 5, 94-017 Łódź, Poland
autor
- Institute of Physics, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27,50-370 Wrocław, Poland
autor
- Department of Dermatology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599-7287, USA
autor
- Department of Dermatology, Medical University of Łódź, ul. Kremieniecka 5, 94-017 Łódź, Poland
autor
- Institute of Physics, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27,50-370 Wrocław, Poland
Bibliografia
- [1] BEN-CHETRIT E., Br. J. Rheumatol. 32 (1993), 396.
- [2] MCCAULIFFE D.P., Lupus 6 (1997), 158.
- [3] MCCAULIFFE D.P., WANG L., SATOH M., REEVES W.H., SMALL D., J. Rheumatol. 24 (1997), 860.
- [4] SIBILIA J., Rev. Rhum. Engl. Ed. 65 (1998), 45.
- [5] GORDON T.P., BOLSTAD A.I., RISCHMUELLER M., JONSSON R., WATERMAN S.A., Autoimmunity 34 (2001), 123.
- [6] RHODES D.A., IHRKE G., REINICKE A.T., MALCHEREK G., TOWEY M., ISENBERG D.A., TROWSDALE J., Immunology 106 (2002), 246.
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- [9] KEECH C.L., GORDON T.P., MCCLUSKEY J., J. Autoimmunity 8 (1995), 699.
- [10] POURMAND N., BLANGE L., RINGERTZ N., PETTERSSON L., Autoimmunity 28 (1998), 225.
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- [12] YELL J.A., WANG L., YIN H., MCCAULIFFE D.P., J. Invest. Dermatol. 107 (1996), 622.
- [13] MCCAULIFFE D.P., SWEENEY A., WOZNIACKA A., Cell. Mol. Biol. Lett. 8 (2003), 133.
- [14] IOANNIDES D., GOLDEN B.D., BUYON J.P., BYSTRYN J.C., Arch. Dermatol. 136 (2000), 340.
- [15] CASCIOLA-ROSEN L.A., ANHALT G., ROSEN A., J. Exp. Med. 179 (1994), 1317.
- [16] LEFEBER W.P., NORRIS D.A., RYAN S.R., HUFF J.C., LEE L.A., KUBO M., BOYCE S.T., KOTZIN B.L., WESTON W.L., J. Clin. Invest. 74 (1984), 1545.
- [17] FURUKAWA F., KASHIHARA-SAWAMI M., LYONS M.B., NORRIS D.A., J. Invest. Dermatol. 94 (1990), 77.
- [18] JONES S.K., Br. J. Dermatol. 126 (1992), 546.
- [19] KAWASHIMA T., ZAPPI E.G., LIEU T.S., SONTHEIMER R.D., Dermatology 189 Suppl 1 (1994), 6.
- [20] OBERHOLZER M., OSTREICHER M., CHRISTEN H., BRUHLMANN M., Histochem. Cell Biol. 105 (1996), 333.
- [21] JAIN B., Digital Image Processing, Springer-Verlag, Berlin Heidelberg 1997, pp. 73–93.
Typ dokumentu
Bibliografia
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