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The main goal of this research was to examine the influence of a sample preparation method on microscopic images obtained in transmission electron microscopy (TEM). The microscopic images of porous silica-based sol-gel materials were further processed with computer-aided methods, so thus to obtain information about distribution of pores. As a stain dopant osmium tetraoxide was used. Sol-gels were prepared from TEOS (tetraethyl orthosilicate) as a precursor and water as a solvent. The material was placed on microscopic grids, pure and with cellulose, or it was embedded in resin and then cut on a microtome. It has been demonstrated that the preparation procedure influences the pores distribution in a sol-gel silica network examined by TEM.
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Tom
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759--766
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bibliogr. 14 poz.
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autor
autor
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- Bio-Optics Group, Institute of Physics, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław
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bwmeta1.element.baztech-article-BPW1-0013-0098