Erythrocyte surface modification due to the pharmacological treatment studied with atomic force microscopy

• Autorzy: Targosz M. , Czuba P. , Szymoński M.
• Języki publikacji: EN

Abstrakty

EN

The influence of some selected pharmacological compounds on the surface structure of human erythrocytes (red blood cells, RBCs) has been studied by means of atomic force microscopy (AFM). The imaging has been done both in the air environment on the fixed cells, and in a liquid (physiological conditions). RBCs are very sensitive to the osmotic changes in the solution, e.g., increasing NaCl concentration in the solution to a value higher than 0.9% leads to the characteristic changes of the erythrocyte from a discoid-like shape to a very irregular one, the so-called “echinocyte”, with a lot of ledges. Both contact and non-contact AFMs have been used to monitor the consecutive stages of RBC surface modification. Imaging the echinocytes immersed in isotonic solution has shown that the changes in RBC cells are fully reversible in the whole range of concentrations used in this investigation. Furthermore, the modification of the erythrocyte surface morphology induced by nifedipine, a drug used in the pharmacological treatment of hypertension, has been studied in this work.

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Optica Applicata
• Rocznik: 2002
• Tom: Vol. 32, nr 3
• Strony: 275--281
• Opis fizyczny: bibliogr. 7 poz.

Twórcy

autor

Targosz M.

autor

Czuba P.

autor

Szymoński M.

• Institute of Physics, Jagiellonian University, ul. Reymonta 4, 30-059 Kraków